[en] Allelopathy can provide sustainable alternatives to herbicides because it is based on specific signals rather than generic toxicity. We show that the allelopathic activity of Spearmint and Watermint is linked with their main compounds, (−)-carvone and (+)-menthofuran, both deriving from (−)-limonene. Germination of Poppy and Cress, and root growth of Arabidopsis thaliana are inhibited by very low concentrations of (−)-carvone, acting even through the gas phase. (+)-Menthofuran is active as well, but at lower efficacy. Using fluorescently tagged marker lines in tobacco BY-2 cells and Arabidopsis roots, we demonstrate a rapid degradation of microtubules and a remodeling of actin filaments in response to (−)-carvone and, to a milder extent, to (+)-menthofuran. This cytoskeletal response is followed by cell death. By means of a Root Chip system, we can follow the tissue dependent response of the cytoskeleton and show a cell-type dependent gradient of sensitivity between meristem and distal elongation zone, accompanied by programmed cell death.
Disciplines :
Agriculture & agronomy Chemistry
Author, co-author :
Hering, Nathalie; Joseph Gottlieb Kölreuter Institute for Plant Sciences (JKIP) , Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany
Schmit, Anne-Catherine; Université de Strasbourg Institut de biologie moléculaire des plantes (IBMP), CNRS, , 67084 Strasbourg, France
Herzog, Etienne; Université de Strasbourg Institut de biologie moléculaire des plantes (IBMP), CNRS, , 67084 Strasbourg, France
Corbin, Louis-Thibault; Université de Strasbourg Institut de biologie moléculaire des plantes (IBMP), CNRS, , 67084 Strasbourg, France
Schmidt-Speicher, Leona; Institute of Microstructure Technology (IMT) , Karlsruhe Institute of Technology, 76344 Eggenstein-Leopoldshafen, Germany
Ahrens, Ralf; Institute of Microstructure Technology (IMT) , Karlsruhe Institute of Technology, 76344 Eggenstein-Leopoldshafen, Germany
Fauconnier, Marie-Laure ; Université de Liège - ULiège > TERRA Research Centre > Chemistry for Sustainable Food and Environmental Systems (CSFES)
Nick, Peter; Joseph Gottlieb Kölreuter Institute for Plant Sciences (JKIP) , Karlsruhe Institute of Technology, 76131 Karlsruhe, Germany
Graduate Funding from the GermanStates (LGF). The microfluidic bioreactor was developed as part of a cooperation project funded by the German Federal Ministry of Education and Science (031B0065B). The study on microtubule
responses in planta was supported by the European Fund for Regional Development (INTERREG V Upper Rhine program, DialogProTec)
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