Effects of Low Intensity Pulsed Ultrasound Stimulation on the Temporal Dynamics of Irradiated Bone Tissue Healing: A Histomorphometric Study in Rabbits.
Biewer, Bob; Kleine-Borgmann, Felix; Hammer, Gaël Pet al.
2022 • In International Journal of Molecular Sciences, 23 (20), p. 12426
bone regeneration; low intensity pulsed ultrasound; osteogenesis; osteoradionecrosis; rabbit; tibia; Animals; Rabbits; Bone Regeneration/physiology; Wound Healing; Ultrasonic Waves; Bone and Bones; Ultrasonic Therapy/methods; Bone Diseases; Inorganic Chemistry; Organic Chemistry; Physical and Theoretical Chemistry; Computer Science Applications; Spectroscopy; Molecular Biology; General Medicine; Catalysis
Abstract :
[en] The present study evaluated the influence of Low-Intensity Pulsed Ultrasound (LIPUS) on the regeneration processes of non-critical-size bone defects in irradiated and non-irradiated rabbit tibias. Bone defects were surgically created on both tibiae of six rabbits. The control group had no additional treatment. In one intervention group, one tibia was irradiated with 15 Gy in a single dose. A second group was treated with LIPUS, and a third with a combination of both treatments. The control samples showed 83.10% ± 17.79% of bone repair after 9 weeks, while the irradiated bone had regenerated significantly less during the same period (66.42% ± 29.36%). The LIPUS treatment on irradiated bones performed a 79.21% ± 21.07% bone fill and could not significantly improve the response compared to the non-treated irradiated specimens. However, LIPUS treatment on non-irradiated bone showed bone formations beyond the size defect (115.91% ± 33.69%), which was a highly significant increase when compared to the control group or any irradiated group. The application of ultrasound to healthy bone produced highly significant and enhanced bone formations with 36.70% more regenerated bone when compared to the same application on irradiated bone. LIPUS vibration stimuli may be considered as a promising complementary treatment approach in non-irradiated bone regeneration procedures to shorten the treatment and enhance bone healing. In irradiated bones, the effect of ultrasound application is less clear, and further studies are needed to refine the dynamics of the present results.
Disciplines :
Dentistry & oral medicine
Author, co-author :
Biewer, Bob ; Department of Periodontology and Oral Surgery, Faculty of Medicine, 4000 Liège, Belgium ; Department of Life Science and Medicine (DLSM), University of Luxembourg, 4365 Esch/Alzette, Luxembourg
Kleine-Borgmann, Felix ; Luxembourg Center of Neuropathology (LCNP), 3555 Dudelange, Luxembourg ; Department of Cancer Research (DoCR), Luxembourg Institute of Health (LIH), 1445 Strassen, Luxembourg
Hammer, Gaël P ; National Center of Pathology (NCP), Laboratoire National de Santé (LNS), 3555 Dudelange, Luxembourg
Rompen, Eric ; Université de Liège - ULiège > Département des sciences dentaires > Chirurgie bucco-dentaire et parodontologie ; Department of Periodontology and Oral Surgery, Faculty of Medicine, 4000 Liège, Belgium
Mittelbronn, Michel ; Department of Life Science and Medicine (DLSM), University of Luxembourg, 4365 Esch/Alzette, Luxembourg ; Luxembourg Center of Neuropathology (LCNP), 3555 Dudelange, Luxembourg ; Department of Cancer Research (DoCR), Luxembourg Institute of Health (LIH), 1445 Strassen, Luxembourg ; National Center of Pathology (NCP), Laboratoire National de Santé (LNS), 3555 Dudelange, Luxembourg ; Luxembourg Centre for Systems Biomedicine (LCSB), University of Luxembourg, 4367 Esch/Alzette, Luxembourg
Quatresooz, Pascale ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Histologie
Language :
English
Title :
Effects of Low Intensity Pulsed Ultrasound Stimulation on the Temporal Dynamics of Irradiated Bone Tissue Healing: A Histomorphometric Study in Rabbits.
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