Ulva lactuca; fatty acids; phenolic compounds; antioxidant activity; enzyme inhibition; anti-diabetic properties; et al. Investigation into the Phytochemical Composition, Antioxidant Properties, and In-Vitro Anti-Diabetic Efficacy of Ulva lactuca
Abstract :
[en] In this research, the chemical compositions of various extracts obtained from Ulva lactuca, a type of green seaweed collected from the Nador lagoon in the northern region of Morocco, were compared. Their antioxidant and anti-diabetic properties were also studied. Using GC-MS technology, the fatty acid content of the samples was analyzed, revealing that palmitic acid, eicosenoic acid, and linoleic acid were the most abundant unsaturated fatty acids present in all samples. The HPLC analysis indicated that sinapic acid, naringin, rutin, quercetin, cinnamic acid, salicylic acid, apigenin, flavone, and flavanone were the most prevalent phenolic compounds. The aqueous extract obtained by maceration showed high levels of polyphenols and flavonoids, with values of 379.67 ± 0.09 mg GAE/g and 212.11 ± 0.11 mg QE/g, respectively. This extract also exhibited an impressive ability to scavenge DPPH radicals, as indicated by its IC50 value of 0.095 ± 0.12 mg/mL. Additionally, the methanolic extract obtained using the Soxhlet method demonstrated antioxidant properties by preventing β-carotene discoloration, with an IC50 of 0.087 ± 0.14 mg/mL. Results from in-vitro studies showed that extracts from U. lactuca were able to significantly inhibit the enzymatic activity of α-amylase and α-glucosidase. Among the various extracts, methanolic extract (S) has been identified as the most potent inhibitor, exhibiting a statistically similar effect to that of acarbose. Furthermore, molecular docking models were used to evaluate the interaction between the primary phytochemicals found in these extracts and the human pancreatic α-amylase and α-glucosidase enzymes. These findings suggest that U. lactuca extracts contain bioactive substances that are capable of reducing enzyme activity more effectively than the commercially available drug, acarbose.
Disciplines :
Agriculture & agronomy Chemistry
Author, co-author :
Ouahabi, Safae; Laboratory of Applied and Environmental Chemistry (LCAE), Faculty of Sciences, Mohammed First University, Oujda, Morocco
Daoudi, Nour; Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Oujda, Morocco ; Higher Institute of Nursing Professions and Health Techniques, Oujda, Morocco
Loukili, El; Euromed Research Center, Euromed Polytechnic School, Euromed University of Fes (UEMF), Morocco
Asmae, Hbika; Laboratory of Applied and Environmental Chemistry (LCAE), Faculty of Sciences, Mohammed First University, Oujda, Morocco
Merzouki, Mohammed; Laboratory of Applied and Environmental Chemistry (LCAE), Faculty of Sciences, Mohammed First University, Oujda, Morocco
Bnouham, Mohamed; Laboratory of Bioresources, Biotechnology, Ethnopharmacology and Health, Faculty of Sciences, Mohammed First University, Oujda, Morocco
Challioui, Allal; Laboratory of Applied and Environmental Chemistry (LCAE), Faculty of Sciences, Mohammed First University, Oujda, Morocco
Hammouti, Belkheir; Euromed Research Center, Euromed Polytechnic School, Euromed University of Fes (UEMF), Morocco
Fauconnier, Marie-Laure ; Université de Liège - ULiège > TERRA Research Centre > Chemistry for Sustainable Food and Environmental Systems (CSFES)
Rhazi, Larbi; Institut Polytechnique UniLaSalle, Université d'Artois, UniLaSalle, Beauvais, France
Gotor, Alicia; Institut Polytechnique UniLaSalle, AGHYLE, UniLaSalle, Beauvais, France
Depeint, Flore; Institut Polytechnique UniLaSalle, Université d'Artois, UniLaSalle, Beauvais, France
Ramdani, Mohammed; Laboratory of Applied and Environmental Chemistry (LCAE), Faculty of Sciences, Mohammed First University, Oujda, Morocco
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