[en] This study was aimed to determine allelic and genotypic frequencies of two single nucleotide polymorphisms (SNP s) located in alpha (1) fucosyltransferase (FUT1) gene and mucin 4 (MUC4) gene, and effects of these SNPs on sperm quality traits of Landrace and Yorkshire pigs. A total of 4771 ejaculates from 63 Landrace and 34 Yorkshire boars have been collected from May 2015 to July 2018 at nucleus breeding pig farm in Vietnam. The sperm quality traits were ejaculate volume, spermatozoa motility, sperm concentration and total number of spermatozoa in ejaculate. Hardy-Weinberg equilibrium was assessed using Fisher exact test. A mixed model was used to test the effects of fixed and random factors on the sperm traits. The fixed factors were genotype, breed, age of boar, month of the year, while boar ID number was included in the model as a random effect, allowing to properly model repeated measurements. These resistant genotypes were absent in Landrace. While the frequency of resistant genotypes AA for FUT1 (0.03) and GG for MUC4 (0.16) was relatively low in Yorkshire boar population. The only detected significant effect of FUT1 and MUC4 on semen quality traits had a positive effect of the resistant genotype GG of MUC4 on sperm motility (P = 0.015). The two SNPs located in FUT1 and MUC4 did not affect sperm traits except sperm motility. Consequently, the selection based on these resistant genotypes does not seem to negatively affect the monitored sperm traits.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Luc, D.D.; Department of Animal Breeding and Genetics, Faculty of Animal Science, Vietnam National University of Agriculture, Hanoi, Viet Nam
Bo, H.X.; Department of Animal Breeding and Genetics, Faculty of Animal Science, Vietnam National University of Agriculture, Hanoi, Viet Nam
Thinh, N.H.; Department of Animal Breeding and Genetics, Faculty of Animal Science, Vietnam National University of Agriculture, Hanoi, Viet Nam
Hanh, H.Q.; Department of Animal Production, Faculty of Animal Science, Vietnam National University of Agriculture, Hanoi, Viet Nam
Phuong, D.T.; Department of Animal Breeding and Genetics, Faculty of Animal Science, Vietnam National University of Agriculture, Hanoi, Viet Nam
Manh, T.X.; Dabaco Nucleus Breeding Pig Company, Viet Nam
Hung, N.V.; Dabaco Nucleus Breeding Pig Company, Viet Nam
Ton, V.D.; Department of Animal Production, Faculty of Animal Science, Vietnam National University of Agriculture, Hanoi, Viet Nam
Farnir, Frédéric ; Université de Liège - ULiège > Département de gestion vétérinaire des Ressources Animales (DRA)
Language :
English
Title :
EFFECTS OF FUT1 AND MUC4 POLYMORPHISMS ON SPERM QUALITY TRAITS OF LANDRACE AND YORKSHIRE PIGS UNDER TROPICAL CONDITIONS IN NORTHERN VIETNAM
Publication date :
August 2022
Journal title :
Journal of Animal and Plant Sciences
ISSN :
1018-7081
Publisher :
Pakistan Agricultural Scientists Forum
Volume :
32
Issue :
4
Pages :
1159 - 1167
Peer reviewed :
Peer reviewed
Funding text :
Funding: This study was funded by FIRST (Fostering Innovation through Research, Science and Technology, Vietnam) project No: 11/FIRST/1.a/VNUA3. Ethical Approval: Not applicable for this type of study in Vietnam.
Balcells, I., A. Castello, A. Mercade, J. L. Noguera, A. Fernandez-Rodriguez, A. Sanchez and A. Tomas (2011). Analysis of porcine MUC4 gene as a candidate gene for prolificacy QTL on SSC13 in an Iberian x Meishan F2 population. BMC Genetics 12:93.
Bao, W. B., L. Ye, Z. Y. Pan, J. Zhu, G. Q. Zhu, X. G. Huang and S. L. Wu (2011). Beneficial genotype of swine FUT1 gene governing resistance to E. coli F18 is associated with important economic traits. J. Genetics 90(2): 315-318.
Cuong, N. V., N. T. Thu, T. T. Thoa, T. X. Hoan, N. T. Thuy and N. T. D. Thuy (2012). Polymorphisms of candidate genes associated with meat quality and disease resistance in indigenous and exotic pig breeds of Vietnam. South African J. Anim. Sci., 42: 221-231.
Domino, S. E., L. Zhang, P. J. Gillespie, T. L. Saunders and J. B. Lowe (2001). Deficiency of reproductive tract alpha(1,2) fucosylated glycans and normal fertility in mice with targeted deletions of the FUT1 or FUT2 alpha(1,2) fucosyltransferase locus. Molecular and Cellular Biology 21(24): 8336-8345.
Edfors, I. and M. Torremorell (2010). Escherichia coli and Salmonella in Pigs. Breeding for disease resistance in farm animals. S. C. Bishop, R. F. E. Axford, F. W. Nicholas and J. B. Owen, CABI: 232-250.
Fontanesi, L., F. Bertolini, S. Dall'Olio, L. Buttazzoni, M. Gallo and V. Russo (2012). Analysis of association between the MUC4 g.8227C>G polymorphism and production traits in Italian heavy pigs using a selective genotyping approach. Anim. Biotech. 23(3): 147-155.
Geraci, C., A. R. Varzandi, G. Schiavo, S. Bovo, A. Ribani, V. J. Utzeri, G. Galimberti, L. Buttazzoni, C. Ovilo, M. Gallo, S. Dall'Olio and L. Fontanesi (2019). Genetic markers associated with resistance to infectious diseases have no effects on production traits and haematological parameters in Italian Large White pigs. Livestock Sci., 223: 32-38.
Guy, S. Z., P. C. Thomson and S. Hermesch (2012). Selection of pigs for improved coping with health and environmental challenges: breeding for resistance or tolerance?. Fron. Genetics 3: 281.
Hong, T. T., N. Q. Linh, B. Ogle and J. E. Lindberg (2006). Survey on the prevalence of diarrhoea in pre-weaning piglets and on feeding systems as contributing risk factors in smallholdings in Central Vietnam. Trop. Anim. Health Prod., 38(5): 397-405.
Jaishankar, S., M. Murugan and H. Gopi (201 8). Semen characteristics of exotic pig breeds. International J. Sci., Environ., Tech., 7(2): 659-662.
Joller, D. (2009). Comparative molecular approaches to identify host determinants mediating adhesion of E. coli F4 strains in pigs. Doctor of Sciences, Institute of Animal Sciences.
Knecht, D., S. ZrodoD and K. DuziDski (2014). The influence of boar breed and season on semen parameters. South African J. Anim. Sci., 44: 1-9.
Kondracki, S. (2003). Breed differences in semen characteristics of boars used in artificial insemination in Poland. Pig News and Information 24(4): 119N-122N.
Liu, Y., X. Mei Yin, R. Wei Xia, Y. Jiu Huo, G. Qiang Zhu, S. Long Wu and W. Bin Bao (2015). Association between the MUC4 g.243A > G polymorphism and immune andproduction traits in Large White pigs. Turkish J. Vet. Anim. Sci., 39: 141-146.
Luc, D. D., T. H. Nguyen, H. X. Bo, N. T. Vinh, T. X. Manh, N. V. Hung, V. D. Ton and F. Farnir (2020). Mutation c.307G>A in FUT1 gene has no effect on production performance of Yorkshire pigs in the tropics: the case of Vietnam. Canadian J. Anim. Sci., 100(3): 426-431.
Luise, D., V. Motta, M. Bertocchi, C. Salvarani, P. Clavenzani, F. Fanelli, U. Pagotto, P. Bosi and P. Trevisi (2019). Effect of Mucine 4 and Fucosyltransferase 1 genetic variants on gut homoeostasis of growing healthy pigs. J. Anim. Physiology & Anim. Nutr.,: 1-12.
Luo, Y., X. Qiu, H. Li and Q. Zhang (2010). Association between the Polymorphism in FUT1 Gene and the Resistance to PWD and ED in Three Pig Breeds. Asian-Austr. J. Anim. Sci., 23(10): 1268-1275.
Meijerink, E., R. Fries, P. Vogeli, J. Masabanda, G. Wigger, C. Stricker, S. Neuenschwander, H. U. Bertschinger and G. Stranzinger (1997). Two alpha(1,2) fucosyltransferase genes on porcine chromosome 6q11 are closely linked to the blood group inhibitor (S) and Escherichia c oli F18 receptor (ECF18R) loci. Mamm Genome 8(10): 736-741.
Meijerink, E., S. Neuenschwander, R. Fries, A. Dinter, H. U. Bertschinger, G. Stranzinger and P. Vogeli (2000). A DNA polymorphism influencing alpha(1,2) fucosyltransferase activity of the pig FUT1 enzyme determines susceptibility of small intestinal epithelium to Escherichia coli F18 adhesion. Immunogenetics 52(1-2): 129-136.
Millette, C. F., R. A. Cardullo, D. R. Armant and G. L. Gerton (1987). Fucosy lation events during mammalian spermatogenesis. Annal of the New York Academy of Science 513: 58-73.
Peng, Q. L., J. Ren, X. M. Yan, X. Huang, H. Tang, Y. Z. Wang, B. Zhang and L. S. Huang (2007). The g.243A>G mutation in intron 17 of MUC4 is significantly associated with susceptibility/resistance to ETEC F4ab/ac infection in pigs. Anim. Genetics 38(4): 397-400.
Prunier, A., M. Heinonen and H. Quesnel (2010). High physiological demands in intensively raised pigs: impact on health and welfare. Animal 4(6): 886-898.
Sambrook, J., E. Fritsch and T. Maniatis (1989). Molecular Cloning: A Laboratory Manual. 2nd edn., New York: Cold Spring Harbor Press.
Savić, R., M. Petrović, D. Radojković, Č. Radović, N. Parunović, M. Pušić and R. Radišić (2013). Variability of ejaculate volume and sperm motility depending on the age and intensity of utilization of boars. Biotechnology in Anim. Husb., 29(4): 641-650.
Stoyanova, S., V. Drobkhlav, S. Metodiev and S. Dimitrov (2010). Genotypes of ESR and FUT1 loci and sperm quality traits of Danube white boars. Zhivotnovudni Nauki 47(4): 52-57.
Tretipskul, C., N. Am-in, P. Tummaruk and M. Techakumphu (2012). Season and Breed Effects on Sperm Production in PRRS Free Boars. The Thai Vet. Medi., 42(4): 471-476.
Tsakmakidis, I. A., T. A. Khalifa and C. M. Boscos (2012). Age-related changes in quality and fertility of porcine semen. Biological Research 45(4): 381-386.
Wang, S. J., W. J. Liu, L. G. Yang, C. A. Sargent, H. B. Liu, C. Wang, X. D. Liu, S. H. Zhao, N. A. Affara, A. X. Liang and S. J. Zhang (2012). Effects of FUT1 gene mutation on resistance to infectious disease. Molecular Biology Reports 39(3): 2805-2810.
Zhu, S., Y. Liu, W. Dong, X. Zheng, G. Zhu, S. Wu and W. Bao (2014). Polymorphism of FUT1 Gene M307 and its Relationship with Partial Immune Indexes and Economic Traits in Yorkshire Pigs. Asian J. Anim. Vet. Advances 9: 253-261.
Zinnatova, F. F., S. K. Shakirov, A. M. Alimov, F. F. Zinnatov and A. V. Zudina (2014). Association of polymorphisms of genes ECRF18/FUT1, MC4R, ESR, RYR1 with the reproductive ability of boars-manufacturers "Kama Bacon Ltd". Issues of Legal Regulation in Veterinary Medicine 4(4-2014): 79-82.
