[en] Four strains of green microalgae (Scenedesmus acutus, Scenedesmus vacuolatus, Chlorella sorokiniana, and Chlamydomonas reinhardtii) were compared to determine growth and pigment composition under photoautotrophic or heterotrophic conditions. Batch growth experiments were performed in multicultivators with online monitoring of optical density. For photoautotrophic growth, light-limited (CO2-sufficient) growth was analyzed under different light intensities during the exponential and deceleration growth phases. The specific growth rate, measured during the exponential phase, and the maximal biomass productivity, measured during the deceleration phase, were not related to each other when different light intensities and different species were considered. This indicates species-dependent photoacclimation effects during cultivation time, which was confirmed by light-dependent changes in pigment content and composition when exponential and deceleration phases were compared. Except for C. reinhardtii, which does not grow on glucose, heterotrophic growth was promoted to similar extents by acetate and by glucose; however, these two substrates led to different pigment compositions. Weak light increased the pigment content during heterotrophy in the four species but was efficient in promoting growth only in S. acutus. C. sorokiniana, and S. vacuolatus exhibited the best potential for heterotrophic biomass productivities, both on glucose and acetate, with carotenoid (lutein) content being the highest in the former.
Disciplines :
Biotechnology
Author, co-author :
Le, Thanh Tung ; Laboratory of Bioenergetics, InBios/PhytoSystems, Department of Life Sciences, University of Liège, Chemin de la Vallée 4, 4000 Liège, Belgium ; Research Institute for Marine Fisheries, 224 Le Lai Street, Ngo Quyen District, Hai Phong City 04000, Vietnam
Corato, Amélie ; Université de Liège - ULiège > Integrative Biological Sciences (InBioS) ; Diagenode, Liège Science Park, Rue du Bois Saint-Jean 3, 4102 Liège, Belgium
Gerards, Thomas ; Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues ; Département de la Recherche et du Développement Technologique, SPW, Place de la Wallonie 1 (B3), 5100 Namur, Belgium
FWB - Fédération Wallonie-Bruxelles [BE] Walloon region [BE] FEDER - Fonds Européen de Développement Régional [BE]
Funding number :
This research was funded by Action de Recherche Concertée, financed by the French Community of Belgium (Wallonia—Brussels Federation), DARKMET proposal, grant number 17/21- 08, and by the Walloon Region and the Fonds Européen de Développement Régional (FEDER),
ALGAE FACTORY proposal, grant number 469780-671950, and by ADV_BIO grant (SPF Économie, P.M.E., Classes moyennes et Énergie, Direction générale de l’Énergie).
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