APX2 Is an Ascorbate Peroxidase-Related Protein that Regulates the Levels of Plastocyanin in Chlamydomonas.

[en] The function of ascorbate peroxidase-related (APX-R) proteins, present in all green photosynthetic eukaryotes, remains unclear. This study focuses on APX-R from Chlamydomonas reinhardtii, namely, ascorbate peroxidase 2 (APX2). We showed that apx2 mutants exhibited a faster oxidation of the photosystem I primary electron donor, P700, upon sudden light increase and a slower re-reduction rate compared to the wild type, pointing to a limitation of plastocyanin. Spectroscopic, proteomic and immunoblot analyses confirmed that the phenotype was a result of lower levels of plastocyanin in the apx2 mutants. The redox state of P700 did not differ between wild type and apx2 mutants when the loss of function in plastocyanin was nutritionally complemented by growing apx2 mutants under copper deficiency. In this case, cytochrome c6 functionally replaces plastocyanin, confirming that lower levels of plastocyanin were the primary defect caused by the absence of APX2. Overall, the results presented here shed light on an unexpected regulation of plastocyanin level under copper-replete conditions, induced by APX2 in Chlamydomonas.

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Caccamo, Anna ^✱; Université de Liège - ULiège > Integrative Biological Sciences (InBioS) ; VIB-VUB Center for Structural Biology, Pleinlaan 2, Brussels 1050, Belgium ; Brussels Center for Redox Biology, Pleinlaan 2, Brussels 1050, Belgium ; Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, Brussels 1050, Belgium

Vega de Luna, Félix ^✱; Genetics and Physiology of Microalgae, InBios/Phytosystems Research Unit, University of Liège, Chemin de la vallée 4, Liège 4000, Belgium

Misztak, Agnieszka ; Université de Liège - ULiège > Département de gestion vétérinaire des Ressources Animales (DRA)

Pyr Dit Ruys, Sébastien ; de Duve Institute and MASSPROT platform, UCLouvain, Avenue Hippocrate 74, Brussels 1200, Belgium

Vertommen, Didier ; de Duve Institute and MASSPROT platform, UCLouvain, Avenue Hippocrate 74, Brussels 1200, Belgium

Cardol, Pierre ; Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues

Messens, Joris ^✱; VIB-VUB Center for Structural Biology, Pleinlaan 2, Brussels 1050, Belgium ; Brussels Center for Redox Biology, Pleinlaan 2, Brussels 1050, Belgium ; Structural Biology Brussels, Vrije Universiteit Brussel, Pleinlaan 2, Brussels 1050, Belgium

Remacle, Claire ^✱; Université de Liège - ULiège > Département des sciences de la vie > Génétique et physiologie des microalgues

^✱ These authors have contributed equally to this work.

Language :

English

Title :

APX2 Is an Ascorbate Peroxidase-Related Protein that Regulates the Levels of Plastocyanin in Chlamydomonas.

Publication date :

02 March 2024

Journal title :

Plant and Cell Physiology

ISSN :

0032-0781

eISSN :

1471-9053

Publisher :

Oxford University Press (OUP), Japan

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://academic.oup.com/pcp/advance-article-pdf/doi/10.1093/pcp/pcae019/57188554/pcae019.pdf

Funders :

F.R.S.-FNRS - Fonds de la Recherche Scientifique
VIB - Vlaams Instituut voor Biotechnologie
FWO - Research Foundation Flanders
EOS - The Excellence Of Science Program

Available on ORBi :

since 30 April 2024

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