cell lysis; centrifugal microfluidics; disk; DNA extraction; lab-on-a-chip; medical diagnostics; micromixer; nucleic acid; rotor; Cell lysis; Centrifugal microfluidics; Disk; Integrated microfluidic systems; Lab-on-chip devices; Magnetic stirrers; Medical diagnostics; Micro mixers; Nucleic acid; Electronic, Optical and Magnetic Materials; Instrumentation; Condensed Matter Physics; Surfaces, Coatings and Films; Metals and Alloys; Electrical and Electronic Engineering; Materials Chemistry
Abstract :
[en] Micromixers are essential microfluidic modules for fabricating integrated lab-on-chip devices for point-of-care applications. Ease of fabrication and integration with other fluidic modules, practicability for different viscosities of the solutions, and high mixing efficiencies are the most important characteristics of a suitable micromixer. In this study, a magnetic stirrer on a rotating micro-structured disk is presented to mix liquids of a wide range of viscosities (up to 42 mPa.s) in low rotational velocities (less than 600 rpm). The concept relies on implementing a small stainless steel rotor aligned on a circular chamber and actuating that by stationary magnets located on the lab frame. The on-disk magnetic stirrer can be implemented in integrated microfluidic platforms without affecting other modules, e.g., valves, to rapidly prepare a homogenous solution in less than 2 s. Moreover, high mixing indexes and the considerable viscous stresses generated in solutions make this approach a convenient choice for the cell lysis process. Observations show that DNA yields in the order of 100 ± 15% relative to conventional lysis protocols can be achieved when the stirrer spins at 200 rpm for 1 min
Disciplines :
Mechanical engineering
Author, co-author :
Dehghan, Amin; School of Mechanical Engineering, Iran University of Science and Technology, Tehran, Iran
Gholizadeh, Ali ; Université de Liège - ULiège > Aérospatiale et Mécanique (A&M)
Navidbakhsh, Mahdi; School of Mechanical Engineering, Iran University of Science and Technology, Tehran, Iran
Sadeghi, Hossein; Molecular Genetics Department, Genomic Research Center, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Pishbin, Esmail; Bio-microfluidics Laboratory, Department of Electrical Engineering and Information Technology, Iranian Research Organization for Science and Technology, Tehran, Iran
Language :
English
Title :
Integrated Microfluidic System for Efficient DNA Extraction Using On-Disk Magnetic Stirrer Micromixer
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