[en] An ideal tissue-engineered dermal substitute should possess angiogenesis potential to promote wound healing, antibacterial activity to relieve the bacterial burden on skin, as well as sufficient porosity for air and moisture exchange. In light of this, a glass-ceramic (GC) has been incorporated into chitosan and gelatin electrospun nanofibers (240-360 nm), which MEFs were loaded on it for healing acceleration. The GC was doped with silver to improve the antibacterial activity. The bioactive nanofibrous scaffolds demonstrated antibacterial and superior antibiofilm activities against Gram-negative and Gram-positive bacteria. The nanofibrous scaffolds were biocompatible, hemocompatible, and promoted cell attachment and proliferation. Nanofibrous skin substitutes with or without Ag-doped GC nanoparticles did not induce an inflammatory response and attenuated LPS-induced interleukin-6 release by dendritic cells. The rate of biodegradation of the nanocomposite was similar to the rate of skin regeneration under in vivo conditions. Histopathological evaluation of full-thickness excisional wounds in BALB/c mice treated with mouse embryonic fibroblasts-loaded nanofibrous scaffolds showed enhanced angiogenesis, and collagen synthesis as well as regeneration of the sebaceous glands and hair follicles in vivo.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Sharifi, Esmaeel ; Cellular and Molecular Research Center, Basic Health Sciences Institute Shahrekord University of Medical Science Shahrekord Iran ; Department of Tissue Engineering and Biomaterials, School of Advanced Medical Sciences and Technologies Hamadan University of Medical Sciences Hamadan Iran
Sadati, Seyede Athar; Cellular and Molecular Research Center, Basic Health Sciences Institute Shahrekord University of Medical Science Shahrekord Iran
Yousefiasl, Satar ; School of Dentistry Hamadan University of Medical Sciences Hamadan Iran
Sartorius, Rossella; Institute of Biochemistry and Cell Biology (IBBC) National Research Council (CNR) Naples Italy
Zafari, Mahdi; National Cell Bank, Pasteur Institute of Iran Tehran Iran
Rezakhani, Leila; Fertility and Infertility Research Center Health Technology Institute, Kermanshah University of Medical Sciences Kermanshah Iran
Alizadeh, Morteza; Department of Tissue Engineering, School of Medicine Shahroud University of Medical Sciences Shahroud Iran
Nazarzadeh Zare, Ehsan ; School of Chemistry Damghan University Damghan Iran
Omidghaemi, Shadi; Cellular and Molecular Research Center, Basic Health Sciences Institute Shahrekord University of Medical Science Shahrekord Iran
Ghanavatinejad, Fatemeh ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques ; Cellular and Molecular Research Center, Basic Health Sciences Institute Shahrekord University of Medical Science Shahrekord Iran
Jami, Mohammad-Saeid; Cellular and Molecular Research Center, Basic Health Sciences Institute Shahrekord University of Medical Science Shahrekord Iran
Salahinejad, Erfan; Faculty of Materials Science and Engineering K. N. Toosi University of Technology Tehran Iran
Samadian, Hadi ; Dental Implants Research Center Hamadan University of Medical Sciences Hamadan Iran
Paiva-Santos, Ana Cláudia ; Department of Pharmaceutical Technology, Faculty of Pharmacy University of Coimbra Coimbra Portugal ; REQUIMTE/LAQV, Group of Pharmaceutical Technology, Faculty of Pharmacy University of Coimbra Coimbra Portugal
De Berardinis, Piergiuseppe; Institute of Biochemistry and Cell Biology (IBBC) National Research Council (CNR) Naples Italy
Shafiee, Abbas ; UQ Diamantina Institute, Translational Research Institute, The University of Queensland Brisbane Queensland Australia
Tay, Franklin R; The Graduate School, Augusta University Augusta Georgia USA
Pourmotabed, Samiramis ; Department of Emergency Medicine, School of Medicine Hamadan University of Medical Sciences Hamadan Iran
Makvandi, Pooyan ; School of Chemistry Damghan University Damghan Iran ; Istituto Italiano di Tecnologia, Centre for Materials Interfaces Pontedera Pisa Italy
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