Pit-1 mediates cell-specific and cAMP-induced transcription of the tilapia GH gene

Amino Acid Sequence; Animals; Base Sequence; Cyclic AMP/*pharmacology; DNA-Binding Proteins/*genetics/metabolism; Gene Expression Regulation/*drug effects; Growth Hormone/*genetics/metabolism; Molecular Sequence Data; Promoter Regions, Genetic/genetics; Rats; Sequence Alignment; Tilapia/*genetics; Transcription Factor Pit-1; Transcription Factors/*genetics/metabolism; Transcription, Genetic/drug effects

Abstract :

[en] Expression of the tilapia growth hormone (tiGH) gene is pituitary-specific and controlled by intracellular cAMP levels. DNaseI protection experiments allowed us to identify four Pit-1 binding sites in the tiGH - 465/ + 19 region. Deletion and mutagenesis analysis revealed that the - 131/+ 19 region, containing two Pit-1 sites, or four copies of the most proximal site tiGHF1 fused to the heterologous Tk promoter, confer high level expression in rat pituitary cells and direct transcription in non-pituitary cells only after expression of rat Pit-1. We show that a tilapia pituitary factor specifically binds to site tiGHF1 and obtained a partial cDNA sequence coding for tilapia Pit-1. The cAMP stimulation is mediated by the proximal (- 131/- 31) promoter region. It is Pit-1-dependent and requires the tiGHF1 site. In addition, four copies of this site confer cAMP inducibility to the Tk promoter in GC cells.

Research Center/Unit :

AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège
Giga-Development and Stem Cells - ULiège

Disciplines :

Biochemistry, biophysics & molecular biology

Author, co-author :

Sekkali, B.

Belayew, A.; University of Leu6en

Bortolussi, M.; Universita di Padova

Martial, Joseph ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire

Muller, Marc ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire

Language :

English

Title :

Pit-1 mediates cell-specific and cAMP-induced transcription of the tilapia GH gene

Publication date :

1999

Journal title :

Molecular and Cellular Endocrinology

ISSN :

0303-7207

eISSN :

1872-8057

Publisher :

North Holland Publishing Company, Amsterdam, Netherlands

Volume :

152

Issue :

1-2

Pages :

111-23

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6T3G-3WMJSXW-D&_user=532038&_rdoc=1&_fmt=&_orig=search&_sort=d&view=c&_acct=C000026659&_version=1&_urlVersion=0&_userid=532038&md5=296dfad6863a1c387e9b52c0aa540fd2

Commentary :

www.elsevier.com

Available on ORBi :

since 04 December 2009

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