Article (Scientific journals)
Pit-1 mediates cell-specific and cAMP-induced transcription of the tilapia GH gene
Sekkali, B.; Belayew, A.; Bortolussi, M. et al.
1999In Molecular and Cellular Endocrinology, 152 (1-2), p. 111-23
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Keywords :
Amino Acid Sequence; Animals; Base Sequence; Cyclic AMP/*pharmacology; DNA-Binding Proteins/*genetics/metabolism; Gene Expression Regulation/*drug effects; Growth Hormone/*genetics/metabolism; Molecular Sequence Data; Promoter Regions, Genetic/genetics; Rats; Sequence Alignment; Tilapia/*genetics; Transcription Factor Pit-1; Transcription Factors/*genetics/metabolism; Transcription, Genetic/drug effects
Abstract :
[en] Expression of the tilapia growth hormone (tiGH) gene is pituitary-specific and controlled by intracellular cAMP levels. DNaseI protection experiments allowed us to identify four Pit-1 binding sites in the tiGH - 465/ + 19 region. Deletion and mutagenesis analysis revealed that the - 131/+ 19 region, containing two Pit-1 sites, or four copies of the most proximal site tiGHF1 fused to the heterologous Tk promoter, confer high level expression in rat pituitary cells and direct transcription in non-pituitary cells only after expression of rat Pit-1. We show that a tilapia pituitary factor specifically binds to site tiGHF1 and obtained a partial cDNA sequence coding for tilapia Pit-1. The cAMP stimulation is mediated by the proximal (- 131/- 31) promoter region. It is Pit-1-dependent and requires the tiGHF1 site. In addition, four copies of this site confer cAMP inducibility to the Tk promoter in GC cells.
Research center :
AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège
Giga-Development and Stem Cells - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Sekkali, B.
Belayew, A.;  University of Leu6en
Bortolussi, M.;  Universita di Padova
Martial, Joseph ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Muller, Marc  ;  Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Language :
English
Title :
Pit-1 mediates cell-specific and cAMP-induced transcription of the tilapia GH gene
Publication date :
1999
Journal title :
Molecular and Cellular Endocrinology
ISSN :
0303-7207
eISSN :
1872-8057
Publisher :
North Holland Publishing Company, Amsterdam, Netherlands
Volume :
152
Issue :
1-2
Pages :
111-23
Peer reviewed :
Peer Reviewed verified by ORBi
Commentary :
www.elsevier.com
Available on ORBi :
since 04 December 2009

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