Amino Acid Sequence; Animals; Artemia/*genetics/metabolism; Base Sequence; Blotting, Southern; *Caenorhabditis elegans Proteins; DNA Restriction Enzymes/metabolism; *Gene Expression; Homeodomain Proteins/*genetics/*metabolism; In Situ Hybridization; Molecular Sequence Data; Reverse Transcriptase Polymerase Chain Reaction; Salt Gland/*metabolism; Time Factors
Abstract :
[en] We characterized the first POU-homeoprotein in a crustacean (designated APH-1 for Artemia POU-Homeoprotein, EMBL Y15070). The amino acid sequence of the APH-1 POU-domain is identical, except for two residues, to that of the two class III POU proteins Cf1-a (Drosophila) and POU-M1 (Bombyx mori). Southern blot analysis suggests that crustaceans have only one class III POU gene. RT-PCR and whole-mount in situ hybridization show that APH-1 mRNA is present in larvae specifically in the salt gland, an organ which is involved in osmoregulation, and disappears in the adult.
Research center :
AFFISH-RC - Applied and Fundamental FISH Research Center - ULiège Giga-Development and Stem Cells - ULiège
Landry, Claire; de Duve Institute of Cellular Pathology and Louvain University Medical School
Loret, Suzanne
Muller, Marc ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Figueroa, Jaime E.; Universidad Austral
Peers, Bernard ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Rentier-Delrue, Françoise ; Université de Liège - ULiège > Département des sciences de la vie > Biologie et génétique moléculaire - GIGA-R : Coordination scientifique
Rousseau, Guy G.; de Duve Institute of Cellular Pathology and Louvain University Medical School
Krauskopf, Manuel; Universidad Austral
Martial, Joseph ; Université de Liège - ULiège > Département des sciences de la vie > GIGA-R : Biologie et génétique moléculaire
Language :
English
Title :
APH-1, a POU homeobox gene expressed in the salt gland of the crustacean Artemia franciscana
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