Changes in bacterial community structure and carbon metabolism in sandy soil under the long-term application of chitin-rich organic material and attapulgite
Zhang, He; Degré, Aurore; De Clerck, Carolineet al.
[en] Continuous expansion of sandy soil deeply threatens crop security and has become one of the main challenges facing modern society. Chitin-rich organic material and attapulgite have been considered efficient materials to improve degraded soil based on their rich nutrient contents and unique structures. However, their potential effects on microbial community and functions in sandy soil are poorly understood. Therefore, we conducted a 4-year field experiment featuring amendment with chitin-rich organic material and attapulgite and investigated the effect of amendments on microbial community diversity, structure and function using amplicons (16S and ITS) and metagenomic sequencing. The four treatments were as follows: (1) CK: no soil amendment; (2) SA: CK + attapulgite amendment; (3) SC: CK + chitin-rich organic material; and (4) SCA: CK + chitin-rich organic material + attapulgite amendment. The results indicated that the concentrations of soil organic carbon (SOC), total nitrogen, available phosphate (AP), available potassium, and enzyme activities increased while pH decreased in the treatments with chitin-rich organic material application (SC and SCA). Microorganisms have different responses to different soil amendments. Bacteria are the main components of microorganisms and their community structure was altered under chitin-rich organic material treatments (SC and SCA). Furthermore, 14 OTUs belonging to five phyla (Proteobacteria, Actinobacteriota, Firmicutes, Gemmatimonadota, and Patescibacteria) related to the decomposition of organic matter were enriched in SC, while 22 OTUs belonging to six phyla (Proteobacteria, Actinobacteriota, Firmicutes, Gemmatimonadota, Patescibacteria, and Cyanobacteria) were enriched significantly in SCA, suggesting that the combination of the two amendments had the potential to further alter the bacterial community compared with a single amendment. The metagenomic analysis revealed a decrease in the relative abundance of genes involved in carbon metabolism in SCA, with soil pH, AP, enzyme activity, SOC, and bacterial community structure identified as primary influencing factors. In conclusion, we conducted a comprehensive analysis of the effects of chitin-rich organic material and attapulgite on microorganisms in sandy soil and found a robust link between soil properties, bacterial community structure, and microbial carbon metabolism function.
Disciplines :
Agriculture & agronomy
Author, co-author :
Zhang, He ; Université de Liège - ULiège > TERRA Research Centre ; State Key Laboratory of Efficient Utilization of Arid and Semi-Arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China
Degré, Aurore ; Université de Liège - ULiège > TERRA Research Centre > Echanges Eau - Sol - Plantes
De Clerck, Caroline ; Université de Liège - ULiège > TERRA Research Centre > Plant Sciences
Li, Shuangshuang; State Key Laboratory of Efficient Utilization of Arid and Semi-Arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China
Lian, Jinshan; State Key Laboratory of Efficient Utilization of Arid and Semi-Arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China
Peng, Yuanyuan; State Key Laboratory of Efficient Utilization of Arid and Semi-Arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China
Sun, Tao; State Key Laboratory of Efficient Utilization of Arid and Semi-Arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China
Luo, Lindan; State Key Laboratory of Efficient Utilization of Arid and Semi-Arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China
Yue, Yanan; State Key Laboratory of Efficient Utilization of Arid and Semi-Arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China
Li, Guihua; State Key Laboratory of Efficient Utilization of Arid and Semi-Arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China
Zhang, Jianfeng; State Key Laboratory of Efficient Utilization of Arid and Semi-Arid Arable Land in Northern China, Institute of Agricultural Resources and Regional Planning, Chinese Academy of Agricultural Sciences, Beijing, China
Language :
English
Title :
Changes in bacterial community structure and carbon metabolism in sandy soil under the long-term application of chitin-rich organic material and attapulgite
NSCF - National Natural Science Foundation of China
Funding text :
This work was jointly supported by the National Natural Science Foundation of China ( 22176215 ), the National Modern Agricultural Industry Technology System ( CARS-23-B18 ), and the Key Research and Development Program of Hainan Province ( ZDYF2021XDNY280 ).
Aklog, Y., Egusa, M., Kaminaka, H., Izawa, H., Morimoto, M., Saimoto, H., Ifuku, S., Protein/caco3/chitin nanofiber complex prepared from crab shells by simple mechanical treatment and its effect on plant growth. Int. J. Mol. Sci., 17, 2016, 1600, 10.3390/ijm7101600.
Ali, I., Yuan, P., Ullah, S., Iqbal, A., Zhao, Q., Liang, H., Khan, A., Imran Zhang, H., Wu, X., Wei, S., Gu, M., Jiang, L., Biochar amendment and nitrogen fertilizer contribute to the changes in soil properties and microbial communities in a paddy field. Front. Microbiol., 13, 2022, 834751, 10.3389/fmicb.2022.834751.
Allison, S.D., Weintraub, M.N., Gartner, T.B., Waldrop, M.P., Evolutionary-Economic Principles as Regulators of Soil Enzyme Production and Ecosystem Function. In: Shukla, G., Varma, A. (Eds) Soil Enzymology. Soil Biology, Vol 22, 2010, Springer, Berlin, Heidelberg, 10.1007/978-3-642-14225-3_12.
Amadou, A., Song, X., Huang, S., Song, A., Tang, Z., Dong, W., Zhao, S., Zhang, B., Yi, K., Fan, F., Effects of long-term organic amendment on the fertility of soil, nodulation, yield, and seed quality of soybean in a soybean-wheat rotation system. J. Soil. Sediment. 21 (2021), 1385–1394, 10.1007/s11368-021-02887-1.
Andreo-Jimenez, B.S.M.N.E., Chitin- and keratin-rich soil amendments suppress rhizoctonia solani disease via changes to the soil microbial community. Appl. Environ. Microbiol. 87 (2021), e00318–e00321, 10.1128/AEM.00318-21.
Bonanomi, G., De Filippis, F., Zotti, M., Idbella, M., Cesarano, G., Al-Rowaily, S., Abd-ElGawad, A., Repeated applications of organic amendments promote beneficial microbiota, improve soil fertility and increase crop yield. Appl. Soil Ecol., 156, 2020, 103714, 10.1016/j.apsoil.2020.103714.
Cai, A., Xu, M., Wang, B., Zhang, W., Liang, G., Hou, E., Luo, Y., Manure acts as a better fertilizer for increasing crop yields than synthetic fertilizer does by improving soil fertility. Soil Tillage Res. 189 (2019), 168–175, 10.1016/j.still.2018.12.022.
Cao, Q., You, B., Liu, W., Xie, L., Jiang, W., Cheng, C., Using soil amendments to reduce microcystin-LR bioaccumulation in lettuce. Environ. Pollut., 292, 2022, 118354, 10.1016/j.envpol.2021.118354.
Chen, D., Wang, X., Carrión, V.J., Yin, S., Yue, Z., Liao, Y., Dong, Y., Li, X., Acidic amelioration of soil amendments improves soil health by impacting rhizosphere microbial assemblies. Soil Biol. Biochem., 167, 2022, 108599, 10.1016/j.soilbio.2022.108599.
Chen, L., Redmile-Gordon, M., Li, J., Zhang, J., Xin, X., Zhang, C., Ma, D., Zhou, Y., Linking cropland ecosystem services to microbiome taxonomic composition and functional composition in a sandy loam soil with 28-year organic and inorganic fertilizer regimes. Appl. Soil Ecol. 139 (2019), 1–9, 10.1016/j.apsoil.2019.03.011.
Cheng, Z., Shi, J., He, Y., Wu, L., Xu, J., Assembly of root-associated bacterial community in cadmium contaminated soil following five-year consecutive application of soil amendments: evidences for improved soil health. J. Hazard. Mater., 426, 2022, 128095, 10.1016/j.jhazmat. 2021.128095.
Clarke, A., Gaston, K.J., Climate, energy and diversity. Proc. R. Soc. B 273 (2006), 2257–2266, 10.1098/rspb.2006.3545.
Cretoiu, M.S., Kielak, A.M., Schluter, A., van Elsas, J.D., Bacterial communities in chitin-amended soil as revealed by 16S rRNA gene based pyrosequencing. Soil Biol. Biochem. 76 (2014), 5–11, 10.1016/j.soilbio.2014.04.027.
De Tender, C., Mesuere, B., Van der Jeugt, F., Haegeman, A., Ruttink, T., Vandecasteele, B., Dawyndt, P., Debode, J., Kuramae, E.E., Peat substrate amended with chitin modulates the N-cycle, siderophore and chitinase responses in the lettuce rhizobiome. Sci. Rep., 9, 2019, 10.1038/s41598-019-46106-x.
Debode, J., De Tender, C., Soltaninejad, S., Van Malderghem, C., Haegeman, A., Van der Linden, I., Cottyn, B., Heyndrickx, M., Maes, M., Chitin mixed in potting soil alters lettuce growth, the survival of zoonotic bacteria on the leaves and associated rhizosphere microbiology. Front. Microbiol., 7, 2016, 10.3389/fmicb.2016.00565.
Dominguez, J., Bacosa, H.P., Chien, M.F., Inoue, C., Enhanced degradation of polycyclic aromatic hydrocarbons (PAHs) in the rhizosphere of sudangrass (sorghum x drummondii). Chemosphere. 234 (2019), 789–795, 10.1016/j.chemosphere.2019.05.290.
Donhauser, J., Qi, W., Bergk Pinto, B., Frey, B., High temperatures enhance the microbial genetic potential to recycle C and N from necromass in high-mountain soils. Glob. Change Biol. 27 (2021), 1365–1386, 10.1111/gcb.15492.
Fierer, N., Ladau, J., Clemente, J.C., Leff, J.W., Owens, S.M., Pollard, K.S., Knight, R., Gilbert, J.A., McCulley, R.L., Reconstructing the microbial diversity and function of pre-agricultural tallgrass prairie soils in the United States. Science. 342 (2013), 621–624, 10.1126/science.1243768.
Fierer, N., Wood, S.A., Bueno De Mesquita, C.P., How microbes can, and cannot, be used to assess soil health. Soil Biol. Biochem., 153, 2021, 108111, 10.1016/j.soilbio.2020.108111.
Garrido-Oter, R., Nakano, R.T., Dombrowski, N., Ma, K., McHardy, A.C., Schulze-Lefert, P., Modular traits of the Rhizobiales root microbiota and their evolutionary relationship with symbiotic rhizobia. Cell Host Microbe 24 (2018), 155–167, 10.1016/j.chom.2018.06.006.
Gupta, S., Graham, D.W., Sreekrishnan, T.R., Ahammad, S.Z., Exploring the impacts of physicochemical characteristics and heavy metals fractions on bacterial communities in four rivers. J. Environ. Manage., 325, 2023, 116453, 10.1016/j.jenvman.2022.116453.
Han, J., Dong, Y., Zhang, M., Chemical fertilizer reduction with organic fertilizer effectively improve soil fertility and microbial community from newly cultivated land in the loess plateau of China. Appl. Soil Ecol., 165, 2021, 103966, 10.1016/j.apsoil.2021.103966.
He, Y., Lu, L., Peng, C., Li, H., Zhang, J., Li, R., Zhou, C., High-yield grass Pennisetum sinese Roxb plantation and organic manure alter bacterial and fungal communities structure in an ecological agriculture farm. AMB Express., 10, 2020, 10.1186/s13568-020-01018-2.
Hu, X., Liu, J., Wei, D., Zhu, P., Cui, X.A., Zhou, B., Chen, X., Jin, J., Liu, X., Wang, G., Effects of over 30-year of different fertilization regimes on fungal community compositions in the black soils of Northeast China. Agric. Ecosyst. Environ. 248 (2017), 113–122, 10.1016/j.agee.2017.07.031.
Hu, X., Gu, H., Liu, J., Wei, D., Zhu, P., Cui, X., Zhou, B., Chen, X., Jin, J., Liu, X., Wang, G., Metagenomics reveals divergent functional profiles of soil carbon and nitrogen cycling under long-term addition of chemical and organic fertilizers in the black soil region. Geoderma., 418, 2022, 115846, 10.1016/j.geoderma.2022.115846.
Huang, J., Hartemink, A.E., Soil and environmental issues in sandy soils. Earth Sci. Rev., 208, 2020, 103295, 10.1016/j.earscirev.2020.103295.
Ji, C., Ye, R., Yin, Y., Sun, X., Ma, H., Gao, R., Reductive soil disinfestation with biochar amendment modified microbial community composition in soils under plastic greenhouse vegetable production. Soil Tillage Res., 218, 2022, 105323, 10.1016/j.still.2022.105323.
Ji, Z., Zhou, J., Zhang, H., Li, G., Zhang, J., Effect of soil conditioners on the soil chemical properties and organic carbon pool of saline –sodic soil. J. Agro-Environ. Sci. 2019:8 (2019), 1759–1767, 10.11654/jaes.2019-0426. (in Chinese).
Jiang, N., Cai, D., He, L., Zhong, N., Wen, H., Zhang, X., Wu, Z., A facile approach to remediate the microenvironment of saline–alkali soil. ACS Sustain. Chem. Eng. 3 (2015), 374–380, 10.1021/sc500785e.
Kang, Y., An, X., Ma, Y., Zeng, S., Jiang, S., Wu, W., Xie, C., Wang, Z., Dong, C., Xu, Y., Shen, Q., Organic amendments alleviate early defoliation and increase fruit yield by altering assembly patterns and of microbial communities and enzymatic activities in sandy pear (Pyrus pyrifolia). AMB Express, 11, 2021, 10.1186/s13568-021-01322-5.
Khodadad, C.L.M., Zimmerman, A.R., Green, S.J., Uthandi, S., Foster, J.S., Taxa-specific changes in soil microbial community composition induced by pyrogenic carbon amendments. Soil Biol. Biochem. 43 (2011), 385–392, 10.1016/j.soilbio.2010.11.005.
Lauber, C.L., Strickland, M.S., Bradford, M.A., Fierer, N., The influence of soil properties on the structure of bacterial and fungal communities across land-use types. Soil Biol. Biochem. 40 (2008), 2407–2415, 10.1016/j.soilbio.2008.05.021.
Li, C., Ma, S., Shao, Y., Ma, S., Zhang, L., Effects of long-term organic fertilization on soil microbiologic characteristics, yield and sustainable production of winter wheat. J. Integr. Agric. 17 (2018), 210–219, 10.1016/S2095-3119(17)61740-4.
Li, T., Gao, J., Bai, L., Wang, Y., Huang, J., Kumar, M., Zeng, X., Influence of green manure and rice straw management on soil organic carbon, enzyme activities, and rice yield in red paddy soil. Soil Tillage Res., 195, 2019, 104428, 10.1016/j.still.2019.104428.
Liang, Y., Xu, L., Bao, J., Firmin, K.A., Zong, W., Attapulgite enhances methane production from anaerobic digestion of pig slurry by changing enzyme activities and microbial community. Renew. Energy 145 (2020), 222–232, 10.1016/j.renene.2019.06.037.
Liu, E., Yan, C., Mei, X., He, W., Bing, S.H., Ding, L., Liu, Q., Liu, S., Fan, T., Long-term effect of chemical fertilizer, straw, and manure on soil chemical and biological properties in Northwest China. Geoderma. 158 (2010), 173–180, 10.1016/j.geoderma.2010.04.029.
Liu, X., Wu, X., Liang, G., Zheng, F., Zhang, M., Li, S., A global meta-analysis of the impacts of no-tillage on soil aggregation and aggregate-associated organic carbon. Land Degrad. Dev. 32 (2021), 5292–5305, 10.1002/ldr.4109.
Liu, X., Yang, J., Tao, J., Yao, R., Integrated application of inorganic fertilizer with fulvic acid for improving soil nutrient supply and nutrient use efficiency of winter wheat in a salt-affected soil. Appl. Soil Ecol., 170, 2022, 104255, 10.1016/j.apsoil.2021.104255.
Luan, H., Gao, W., Huang, S., Tang, J., Li, M., Zhang, H., Chen, X., Partial substitution of chemical fertilizer with organic amendments affects soil organic carbon composition and stability in a greenhouse vegetable production system. Soil Tillage Res. 191 (2019), 185–196, 10.1016/j.still.2019.04.009.
Mangalassery, S., Kalaivanan, D., Philip, P.S., Effect of inorganic fertilisers and organic amendments on soil aggregation and biochemical characteristics in a weathered tropical soil. Soil Tillage Res. 187 (2019), 144–151, 10.1016/j.still.2018.12.008.
Morrissey, E.M., Kane, J., Tripathi, B.M., Rion, M.S.I., Hungate, B.A., Franklin, R., Walter, C., Sulman, B., Brzostek, E., Carbon acquisition ecological strategies to connect soil microbial biodiversity and carbon cycling. Soil Biol. Biochem., 177, 2023, 108893, 10.1016/j.soilbio.2022.108893.
Niaz, S., Wehr, J.B., Dalal, R.C., Kopittke, P.M., Menzies, N.W., Organic amendments and gypsum reduce dispersion and increase aggregation of two sodic Vertisols. Geoderma., 425, 2022, 116047, 10.1016/j.geoderma.2022.116047.
Pang, Z., Dong, F., Liu, Q., Lin, W., Hu, C., Yuan, Z., Soil metagenomics reveals effects of continuous sugarcane cropping on the structure and functional pathway of Rhizospheric microbial community. Front. Microbiol., 12, 2021, 10.3389/fmicb.2021.627569.
Qi, Y., Liu, H., Zhang, B., Geng, M., Cai, X., Wang, J., Wang, Y., Investigating the effect of microbial inoculants Frankia F1 on growth-promotion, rhizosphere soil physicochemical properties, and bacterial community of ginseng. Appl. Soil Ecol., 172, 2022, 104369, 10.1016/j.apsoil.2021.104369.
Qiu, Q., Wang, H., Zhang, Q., Said Mgelwa, A., Zhu, B., Hu, Y., Negative priming effect from tree leaf and root residues with contrasting chemical composition. Geoderma., 427, 2022, 116118, 10.1016/j.geoderma.2022.116118.
Rafiq, M.K., Joseph, S.D., Li, F., Bai, Y., Shang, Z., Rawal, A., Hook, J.M., Munroe, P.R., Donne, S., Taherymoosavi, S., Mitchell, D.R.G., Pace, B., Mohammed, M., Horvat, J., Marjo, C.E., Wagner, A., Wang, Y., Ye, J., Long, R., Pyrolysis of attapulgite clay blended with yak dung enhances pasture growth and soil health: characterization and initial field trials. Sci. Total Environ. 607-608 (2017), 184–194, 10.1016/j.scitotenv.2017.06.186.
Randall, T.E., Fernandez-Bayo, J.D., Harrold, D.R., Achmon, Y., Hestmark, K.V., Gordon, T.R., Stapleton, J.J., Simmons, C.W., VanderGheynst, J.S., Changes of fusarium oxysporum f.sp. lactucae levels and soil microbial community during soil biosolarization using chitin as soil amendment. PloS One, 15, 2020, e232662, 10.1371/journal.pone.0232662.
Rkhaila, A., Chtouki, T., Erguig, H., El Haloui, N., Ounine, K., Chemical proprieties of biopolymers (chitin/chitosan) and their synergic effects with endophytic Bacillus species: unlimited applications in agriculture. Molecules., 26, 2021, 1117, 10.3390/molecules26041117.
Saiya-Cork, K.R., Sinsabaugh, R.L., Zak, D.R., The effects of long term nitrogen deposition on extracellular enzyme activity in an Acer saccharum forest soil. Soil Biol. Biochem. 34 (2002), 1309–1315, 10.1016/S0038-0717(02)00074-3.
Shamshina, J.L., Kelly, A., Oldham, T., Rogers, R.D., Agricultural uses of chitin polymers. Environ. Chem. Lett. 18 (2020), 53–60, 10.1007/s10311-019-00934-5.
Sharp, R., A review of the applications of chitin and its derivatives in agriculture to modify plant-microbial interactions and improve crop yields. Agronomy. 3 (2013), 757–793, 10.3390/agronomy3040757.
Song, D., Dai, X., Guo, T., Cui, J., Zhou, W., Huang, S., Shen, J., Liang, G., He, P., Wang, X., Zhang, S., Organic amendment regulates soil microbial biomass and activity in wheat-maize and wheat-soybean rotation systems. Agric. Ecosyst. Environ., 333, 2022, 107974, 10.1016/j.agee.2022.107974.
Soria, R., Rodríguez-Berbel, N., Sánchez-Cañete, E.P., Villafuerte, A.B., Ortega, R., Miralles, I., Organic amendments from recycled waste promote short-term carbon sequestration of restored soils in drylands. J. Environ. Manage., 327, 2023, 116873, 10.1016/j. jenvman.2022.116873.
Sun, R., Chen, Y., Han, W., Dong, W., Zhang, Y., Hu, C., Liu, B., Wang, F., Different contribution of species sorting and exogenous species immigration from manure to soil fungal diversity and community assemblage under long-term fertilization. Soil Biol. Biochem., 151, 2020, 108049, 10.1016/j.soilbio.2020.108049.
Tang, Y., Zhan, T., Fan, G., Huang, J., Zhou, Y., Yuan, X., Li, K., Chen, S., Zhao, X., Hu, C., Selenium combined with chitin reduced phosphorus leaching in soil with pomelo by driving soil phosphorus cycle via microbial community. J. Environ. Chem. Eng., 10, 2022, 107060, 10.1016/j.jece.2021.107060.
Tao, Y., Shen, L., Han, S., Li, Z., Cui, Y., Lin, Y., Qu, J., Zhang, Y., Metagenomic study of carbon metabolism in black soil microbial communities under lead-lanthanum stress. J. Hazard. Mater., 446, 2023, 130666, 10.1016/j.jhazmat.2022.130666.
Vance, E.D., Brookes, P.C., Jenkinson, D.S., An extraction method for measuring soil microbial biomass C. Soil Biol. Biochem. 19 (1987), 703–707, 10.1016/0038-0717(87)90052-6.
Wang, H., Zhang, W., Chen, L., Xu, Q., Jiang, Y., Sun, B., Biochar induced negative priming effect on soil organic carbon mineralisation by changing the microbial community structure across plant growth stages. J. Soil. Sediment. 20 (2020), 3340–3350, 10.1007/s11368-020-02662-8.
Wang, J., Fu, X., Ghimire, R., Sainju, U.M., Jia, Y., Zhao, F., Responses of soil bacterial community and enzyme activity to organic matter components under long-term fertilization on the loess plateau of China. Appl. Soil Ecol., 166, 2021, 103992, 10.1016/j.apsoil.2021.103992.
Wieczorek, A.S., Hetz, S.A., Kolb, S., Microbial responses to chitin and chitosan in oxic and anoxic agricultural soil slurries. Biogeosciences. 11 (2014), 3339–3352, 10.5194/bg-11-3339-2014.
Winkler, A., Dominguez-Nuñez, J., Aranaz, I., Poza-Carrión, C., Ramonell, K., Somerville, S., Berrocal-Lobo, M., Short-chain chitin oligomers: promoters of plant growth. Mar. Drugs, 15, 2017, 40, 10.3390/md15020040.
Wu, F., Jiao, S., Hu, J., Wu, X., Wang, B., Shen, G., Yang, Y., Tao, S., Wang, X., Stronger impacts of long-term relative to short-term exposure to carbon nanomaterials on soil bacterial communities. J. Hazard. Mater., 410, 2021, 124550, 10.1016/j.jhazmat.2020.124550.
Wu, W., Lin, Z., Zhu, X., Li, G., Zhang, W., Chen, Y., Ren, L., Luo, S., Lin, H., Zhou, H., Huang, Y., Yang, R., Xie, Y., Wang, X., Zhen, Z., Zhang, D., Improved tomato yield and quality by altering soil physicochemical properties and nitrification pr-ocesses in the combined use of organic-inorganic fertilizers. Eur. J. Soil Biol., 109, 2022, 103384, 10.1016/j.ejsobi.2022.103384.
Xiao, E., Ning, Z., Xiao, T., Sun, W., Jiang, S., Soil bacterial community functions and distribution after mining disturbance. Soil Biol. Biochem., 157, 2021, 108232, 10.1016/j.soilbio.2021.108232.
Xiong, C., Singh, B.K., He, J., Han, Y., Li, P., Wan, L., Meng, G., Liu, S., Wang, J., Wu, C., Ge, A., Zhang, L., Plant developmental stage drives the differentiation in ecological role of the maize microbiome. Microbiome., 9, 2021, 10.1186/s40168-021-01118-6.
Xue, W., Han, Y., Tan, J., Wang, Y., Wang, G., Wang, H., Effects of Nanochitin on the enhancement of the grain yield and quality of winter wheat. J. Agric. Food Chem. 66 (2018), 6637–6645, 10.1021/acs.jafc.7b00641.
Xun, W., Yan, R., Ren, Y., Jin, D., Xiong, W., Zhang, G., Cui, Z., Xin, X., Zhang, R., Grazing-induced microbiome alterations drive soil organic carbon turnover and productivity in meadow steppe. Microbiome., 6, 2018, 10.1186/s40168-018-0544-y.
Yang, S., Li, C., Xu, C., Wu, D., Wang, J., Zhang, Y., Ai, Y., Li, H., Effects of add-ing straw and Attapulgite on soil structure and carbon and nitrogen contents of old Yellow River course. Bull. Soil Water Conserv. 2 (2020), 199–224, 10.13961/j.cnki.stbctb.2020.02.029. (in Chinese).
Yang, T., Xing, X., Gao, Y., Ma, X., An environmentally friendly soil amendment for enhancing soil water availability in drought-prone soils. Agronomy., 12, 2022, 133, 10.3390/agronomy12010133.
Yao, Q., Li, Z., Song, Y., Wright, S.J., Guo, X., Tringe, S.G., Tfaily, M.M., Paša-Tolić, L., Hazen, T.C., Turner, B.L., Mayes, M.A., Pan, C., Community proteogenomics reveals the systemic impact of phosphorus availability on microbial functions in tropical soil. Nat. Ecol. Evol. 2 (2018), 499–509, 10.1038/s41559-017-0463-5.
Yu, Z., Ling, L., Singh, B.P., Luo, Y., Xu, J., Gain in carbon: deciphering the abiotic and biotic mechanisms of biochar-induced negative priming effects in contrasting soils. Sci. Total Environ., 746, 2020, 141057, 10.1016/j.scitotenv.2020.141057.
Zhan, T., Hu, C., Kong, Q., Shi, G., Tang, Y., Zhou, Y., Guo, Z., Zhai, H., Xiao, X., Zhao, X., Chitin combined with selenium reduced nitrogen loss in soil and improved nitrogen uptake efficiency in Guanxi pomelo orchard. Sci. Total Environ., 799, 2021, 149414, 10.1016/j.scitotenv.2021.149414.
Zhang, H., Wang, S., Zhang, J., Tian, C., Luo, S., Biochar application enhances microbial interactions in mega-aggregates of farmland black soil. Soil Tillage Res., 213, 2021, 105145, 10.1016/j.still.2021.105145.
Zhang, H., Yang, J., Zhou, J., Li, G., Zhang, J., Effects of organic and inorganic amendments on aggregation and crop yields in sandy fluvo-aquic soil. Plant Nutr. Fert. Sci. 27 (2021), 791–801, 10.11674/zwyf.20576. (in chinese).
Zhang, Y., Ye, C., Su, Y., Peng, W., Lu, R., Liu, Y., Huang, H., He, X., Yang, M., Zhu, S., Soil acidification caused by excessive application of nitrogen fertilizer aggravates soil-borne diseases: evidence from literature review and field trials. Agric. Ecosyst. Environ., 340, 2022, 108176, 10.1016/j.agee.2022.108176.
Zheng, J., Zhang, J., Gao, L., Wang, R., Gao, J., Dai, Y., Li, W., Shen, G., Kong, F., Zhang, J., Effect of straw biochar amendment on tobacco growth, soil properties, and rhizosphere bacterial communities. Sci. Rep., 11, 2021, 10.1038/s41598-021-00168-y.
Zheng, T., Zhang, J., Tang, C., Liao, K., Guo, L., Positive and negative priming effects in an ultisol in relation to aggregate size class and biochar level. Soil Tillage Res., 208, 2021, 104874, 10.1016/j.still.2020.104874.
Zhou, J., Zhang, H., Yang, J., Li, G., Zhang, J., Effects of continuous application of soil amendments on fluvo aquic soil fertility and active organic carbon components. Sci. Agric. Sin. 53 (2020), 3307–3318, 10.3864/j.issn.0578-1752.2020.16.009. (in Chinese).
