[en] Infection with bovine leukemia virus (BLV) leads to enzootic bovine leukosis, the most prevalent neoplastic disease in cattle. Due to the lack of commercially available vaccines, reliable eradication of the disease can be achieved through the testing and elimination of BLV antibody-positive animals. In this study, we developed a novel competitive ELISA (cELISA) to detect antibodies against BLV capsid protein p24. Recombinant p24 protein expressed by Escherichia coli, in combination with the monoclonal antibody 2G11 exhibiting exceptional performance, was used for the establishment of the cELISA. Receiver-operating characteristic curve analysis showed that the sensitivity and specificity of the assay were 98.85 % and 98.13 %, respectively. Furthermore, the established cELISA was specific for detecting BLV-specific antibodies, without cross-reactivity to antisera for six other bovine viruses. Significantly, experimental infection of cattle and sheep with BLV revealed that the cELISA accurately monitors seroconversion. In a performance evaluation, the established cELISA displayed a high agreement with Western blotting and the commercial BLV gp51 cELISA kit in the detection of 242 clinical samples, respectively. In conclusion, the novel p24 cELISA exhibited the potential to be a reliable and efficient diagnostic tool for BLV serological detection with a broad application prospect.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Wang, Jing ; Université de Liège - ULiège > TERRA Research Centre ; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China
Sun, Chao; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China
Hu, Zhe; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China
Wang, Fangzhou ; Université de Liège - ULiège > TERRA Research Centre ; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China
Chang, Jitao; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China, Institute of Western Agriculture, the Chinese Academy of Agricultural Sciences, Changji 831100, China
Gao, Ming; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China
Ye, Dandan ; Université de Liège - ULiège > TERRA Research Centre ; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China
Jia, Qi; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China
Zou, Hui; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China
Willems, Luc ; Université de Liège - ULiège > GIGA > GIGA Cancer - Cellular and Molecular Epigenetics
Jiang, Zhigang; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China. Electronic address: jiangzhigang@caas.cn
Yin, Xin; State Key Laboratory for Animal Disease Control and Prevention, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Harbin 150069, China. Electronic address: yinxin@caas.cn
Language :
English
Title :
Development of a novel monoclonal antibody-based competitive ELISA for antibody detection against bovine leukemia virus.
Publication date :
13 April 2024
Journal title :
International Journal of Biological Macromolecules
Burny, A., Cleuter, Y., Kettmann, R., Mammerickx, M., Marbaix, G., Portetelle, D., van den Broeke, A., Willems, L., Thomas, R., Bovine leukaemia: facts and hypotheses derived from the study of an infectious cancer. Vet. Microbiol. 17 (1988), 197–218, 10.1016/0378-1135(88)90066-1.
I, S., D, L., Pathobiology of bovine leukemia virus. Vet. Res., 25, 1994 https://pubmed.ncbi.nlm.nih.gov/7889034/ (accessed January 20, 2024).
Bartlett, P.C., Norby, B., Byrem, T.M., Parmelee, A., Ledergerber, J.T., Erskine, R.J., Bovine leukemia virus and cow longevity in Michigan dairy herds. J. Dairy Sci. 96 (2013), 1591–1597, 10.3168/jds.2012-5930.
Norby, B., Bartlett, P.C., Byrem, T.M., Erskine, R.J., Effect of infection with bovine leukemia virus on milk production in Michigan dairy cows. J. Dairy Sci. 99 (2016), 2043–2052, 10.3168/jds.2015-10089.
Selim, A., Marawan, M.A., Ali, A.-F., Manaa, E., AbouelGhaut, H.A., Seroprevalence of bovine leukemia virus in cattle, buffalo, and camel in Egypt. Trop. Anim. Health Prod. 52 (2020), 1207–1210, 10.1007/s11250-019-02105-8.
Rodríguez, S.M., Florins, A., Gillet, N., De Brogniez, A., Sánchez-Alcaraz, M.T., Boxus, M., Boulanger, F., Gutiérrez, G., Trono, K., Alvarez, I., Vagnoni, L., Willems, L., Preventive and therapeutic strategies for bovine leukemia virus: lessons for HTLV. Viruses 3 (2011), 1210–1248, 10.3390/v3071210.
Bauermann, F.V., Ridpath, J.F., Dargatz, D.A., Bovine leukemia virus seroprevalence among cattle presented for slaughter in the United States. J. Vet. Diagn. Investig. Off. Publ. Am. Assoc. Vet. Lab. Diagn. Inc 29 (2017), 704–706, 10.1177/1040638717702183.
Ott, S.L., Johnson, R., Wells, S.J., Association between bovine-leukosis virus seroprevalence and herd-level productivity on US dairy farms. Prev. Vet. Med. 61 (2003), 249–262, 10.1016/j.prevetmed.2003.08.003.
Polat, M., Takeshima, S., Aida, Y., Epidemiology and genetic diversity of bovine leukemia virus. Virol. J., 14, 2017, 209, 10.1186/s12985-017-0876-4.
Murakami, K., Kobayashi, S., Konishi, M., Kameyama, K., Tsutsui, T., Nationwide survey of bovine leukemia virus infection among dairy and beef breeding cattle in Japan from 2009-2011. J. Vet. Med. Sci. 75 (2013), 1123–1126, 10.1292/jvms.12-0374.
Ma, B.-Y., Gong, Q.-L., Sheng, C.-Y., Liu, Y., Ge, G.-Y., Li, D.-L., Diao, N.-C., Shi, K., Li, J.-M., Sun, Z.-B., Zong, Y., Leng, X., Du, R., Prevalence of bovine leukemia in 1983–2019 in China: a systematic review and meta-analysis. Microb. Pathog., 150, 2021, 104681, 10.1016/j.micpath.2020.104681.
Khalilian, M., Hosseini, S.M., Madadgar, O., Bovine leukemia virus detected in the breast tissue and blood of Iranian women. Microb. Pathog., 135, 2019, 103566, 10.1016/j.micpath.2019.103566.
Mendoza, W., Isaza, J.P., López, L., López-Herrera, A., Gutiérrez, L.A., Bovine leukemia virus detection in humans: a systematic review and meta-analysis. Virus Res., 335, 2023, 199186, 10.1016/j.virusres.2023.199186.
Robinson, L.A., Jaing, C.J., Pierce Campbell, C., Magliocco, A., Xiong, Y., Magliocco, G., Thissen, J.B., Antonia, S., Molecular evidence of viral DNA in non-small cell lung cancer and non-neoplastic lung. Br. J. Cancer 115 (2016), 497–504, 10.1038/bjc.2016.213.
A. Burny, Y. Cleuter, Bovine Leukaemia: Facts and Hypotheses Derived from the Study of an Infectious Cancer, (n.d.).
Monti, G.E., Frankena, K., Survival analysis on aggregate data to assess time to sero-conversion after experimental infection with bovine leukemia virus. Prev. Vet. Med. 68 (2005), 241–262, 10.1016/j.prevetmed.2005.01.009.
De Boer, G.F., Boerrigter, H.M., Akkermans, J.P.W.M., Brenner, J., Use of milk samples and monoclonal antibodies directed against BLV-p24 to identify cattle infected with bovine leukemia virus (BLV). Vet. Immunol. Immunopathol. 22 (1989), 283–292, 10.1016/0165-2427(89)90015-9.
Simard, C., Richardson, S., Dixon, P., Bélanger, C., Maxwell, P., Enzyme-linked immunosorbent assay for the diagnosis of bovine leukosis: comparison with the agar gel immunodiffusion test approved by the Canadian Food Inspection Agency. Can. J. Vet. Res. Rev. Can. Rech. Veterinaire 64 (2000), 101–106.
Kuczewski, A., Orsel, K., Barkema, H.W., Kelton, D.F., Hutchins, W.A., van der Meer, F.J.U.M., Short communication: evaluation of 5 different ELISA for the detection of bovine leukemia virus antibodies. J. Dairy Sci. 101 (2018), 2433–2437, 10.3168/jds.2017-13626.
Gillet, N., Florins, A., Boxus, M., Burteau, C., Nigro, A., Vandermeers, F., Balon, H., Bouzar, A.B., Defoiche, J., Burny, A., Reichert, M., Kettmann, R., Willems, L., Mechanisms of leukemogenesis induced by bovine leukemia virus: prospects for novel anti-retroviral therapies in human. Retrovirology, 4, 2007, 10.1186/1742-4690-4-18.
Kakker, N.K., Mikhailov, M.V., Jones, I.M., Roy, P., Comparative analysis of the roles of simian immunodeficiency and bovine leukemia virus matrix proteins in Gag assembly in insect cells. Virology 299 (2002), 48–55, 10.1006/viro.2002.1441.
Buehring, G.C., DeLaney, A., Shen, H., Chu, D.L., Razavian, N., Schwartz, D.A., Demkovich, Z.R., Bates, M.N., Bovine leukemia virus discovered in human blood. BMC Infect. Dis., 19, 2019, 297, 10.1186/s12879-019-3891-9.
Gc, B., Hm, S., Hm, J., Dl, J., M, H., G, B., Exposure to bovine leukemia virus is associated with breast cancer: a case-control study. PLoS One, 10, 2015, 10.1371/journal.pone.0134304.
Suárez Archilla, G., Gutiérrez, G., Camussone, C., Calvinho, L., Abdala, A., Alvarez, I., Petersen, M., Franco, L., Destefano, G., Monti, G., Jacques, J.-R., Joris, T., Willems, L., Trono, K., A safe and effective vaccine against bovine leukemia virus. Front. Immunol., 13, 2022, 980514, 10.3389/fimmu.2022.980514.
Gutiérrez, G., Rodríguez, S., De Brogniez, A., Gillet, N., Golime, R., Burny, A., Jaworski, J.-P., Alvarez, I., Vagnoni, L., Trono, K., Willems, L., Vaccination against δ-retroviruses: the bovine leukemia virus paradigm. Viruses 6 (2014), 2416–2427, 10.3390/v6062416.
Yang, Y., Fan, W., Mao, Y., Yang, Z., Lu, G., Zhang, R., Zhang, H., Szeto, C., Wang, C., Bovine leukemia virus infection in cattle of China: association with reduced milk production and increased somatic cell score. J. Dairy Sci. 99 (2016), 3688–3697, 10.3168/jds.2015-10580.
Yakobson, B., Brenner, J., Ungar-Waron, H., Trainin, Z., Cellular immune response cytokine expression during the initial stage of bovine leukemia virus (BLV) infection determines the disease progression to persistent lymphocytosis. Comp. Immunol. Microbiol. Infect. Dis. 23 (2000), 197–208, 10.1016/s0147-9571(99)00074-0.
Gutiérrez, S., Ceriani, C., Juliarena, M., Ferrer, J.F., Host soluble factors that regulate the synthesis of the major core protein of the bovine leukemia virus (BLV) in a naturally infected neoplastic B-cell line. Vet. Immunol. Immunopathol. 131 (2009), 246–258, 10.1016/j.vetimm.2009.04.017.
Bai, L., Yokoyama, K., Watanuki, S., Ishizaki, H., Takeshima, S., Aida, Y., Development of a new recombinant p24 ELISA system for diagnosis of bovine leukemia virus in serum and milk. Arch. Virol. 164 (2019), 201–211, 10.1007/s00705-018-4058-5.
Harms, J.S., Splitter, G.A., Loss of MHC I transcription trans-activator in the bovine B-LCL, BL3.1. Vet. Immunol. Immunopathol. 51 (1996), 39–54, 10.1016/0165-2427(95)05503-7.
Zaghawa, A., Beier, D., Abd El-Rahim, I.H.A., Karim, I., El-ballal, S., Conraths, F.J., Marquardt, O., An outbreak of enzootic bovine leukosis in upper Egypt: clinical, laboratory and molecular-epidemiological studies. J. Vet. Med. B Infect. Dis Vet. Public Health 49 (2002), 123–129, 10.1046/j.1439-0450.2002.00517.x.
Polat, M., Ohno, A., Takeshima, S.-N., Kim, J., Kikuya, M., Matsumoto, Y., Mingala, C.N., Onuma, M., Aida, Y., Detection and molecular characterization of bovine leukemia virus in Philippine cattle. Arch. Virol. 160 (2015), 285–296, 10.1007/s00705-014-2280-3.
Options for the Control of Bovine Leukemia Virus in Dairy Cattle - PubMed, (n.d.). https://pubmed.ncbi.nlm.nih.gov/24697767/ (accessed March 14, 2024).
Lairmore, M.D., Animal models of bovine leukemia virus and human T-lymphotrophic virus type-1: insights in transmission and pathogenesis. Annu. Rev. Anim. Biosci. 2 (2014), 189–208, 10.1146/annurev-animal-022513-114117.
Kohara, J., Takeuchi, M., Hirano, Y., Sakurai, Y., Takahashi, T., Vector control efficacy of fly nets on preventing bovine leukemia virus transmission. J. Vet. Med. Sci. 80 (2018), 1524–1527, 10.1292/jvms.18-0199.
Mammerickx, M., Palm, R., Portetelle, D., Burny, A., Experimental transmission of enzootic bovine leukosis to sheep: latency period of the tumoral disease. Leukemia 2 (1988), 103–107.
Radke, K., Grossman, D., Kidd, L.C., Humoral immune response of experimentally infected sheep defines two early periods of bovine leukemia virus replication. Microb. Pathog. 9 (1990), 159–171, 10.1016/0882-4010(90)90019-M.
Willems, L., Kettmann, R., Reichert, M., Cantor, G.H., Protective effects of a live attenuated bovine leukaemia virus vaccine with deletion in the R3 and G4 genes. J. Gen. Virol. 81 (2000), 965–969, 10.1099/0022-1317-81-4-965.
Florins, A., Cell dynamics and immune response to BLV infection: a unifying model. Front. Biosci., 12, 2007, 1520, 10.2741/2165.
Willems, L., Gatot, J.S., Mammerickx, M., Portetelle, D., Burny, A., Kerkhofs, P., Kettmann, R., The YXXL signalling motifs of the bovine leukemia virus transmembrane protein are required for in vivo infection and maintenance of high viral loads. J. Virol. 69 (1995), 4137–4141, 10.1128/jvi.69.7.4137-4141.1995.
