[en] [en] INTRODUCTION: Alginate oligosaccharide (AOS), as a natural non-toxic plant extract, has been paid more attention in recent years due to its strong antioxidant, anti-inflammatory, and even anti-cancer properties. However, the mechanism by which AOS affects animal reproductive performance is still unclear.
METHODS: The purpose of this study is to use multi-omics technology to analyze the effects of AOS in extending the service lifespan of aging boars.
RESULTS: The results showed that AOS can significantly improve the sperm motility (p < 0.05) and sperm validity rate (p < 0.001) of aging boars and significantly reduce the abnormal sperm rate (p < 0.01) by increasing the protein levels such as CatSper 8 and protein kinase A (PKA) for semen quality. At the same time, AOS significantly improved the testosterone content in the blood of boars (p < 0.01). AOS significantly improved fatty acids such as adrenic acid (p < 0.05) and antioxidants such as succinic acid (p < 0.05) in sperm metabolites, significantly reducing harmful substances such as dibutyl phthalate (p < 0.05), which has a negative effect on spermatogenesis. AOS can improve the composition of intestinal microbes, mainly increasing beneficial bacteria Enterobacter (p = 0.1262) and reducing harmful bacteria such as Streptococcus (p < 0.05), Prevotellaceae_UCG-001 (p < 0.05), and Prevotellaceae_NK3B31_group (p < 0.05). Meanwhile, short-chain fatty acids in feces such as acetic acid (p < 0.05) and butyric acid (p < 0.05) were significantly increased. Spearman correlation analysis showed that there was a close correlation among microorganisms, sperm metabolites, and sperm parameters.
DISCUSSION: Therefore, the data indicated that AOS improved the semen quality of older boars by improving the intestinal microbiota and sperm metabolome. AOS can be used as a feed additive to solve the problem of high elimination rate in large-scale boar studs.
Disciplines :
Animal production & animal husbandry Microbiology
Author, co-author :
Zhou, Yexun ; Université de Liège - ULiège > TERRA Research Centre ; State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
Wei, Zeou; State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
Tan, Jiajian; YangXiang Joint Stock Company, Animal Nutrition Institute, Guigang, China
Sun, Haiqing; YangXiang Joint Stock Company, Animal Nutrition Institute, Guigang, China
Jiang, Haidi; YangXiang Joint Stock Company, Animal Nutrition Institute, Guigang, China
Gao, Yang; College of Life Science, Baicheng Normal University, Baicheng, Jilin, China
Zhang, Hongfu; State Key Laboratory of Animal Nutrition, Institute of Animal Sciences, Chinese Academy of Agricultural Sciences, Beijing, China
Schroyen, Martine ; Université de Liège - ULiège > Département GxABT > Animal Sciences (AS)
Language :
English
Title :
Alginate oligosaccharide extends the service lifespan by improving the sperm metabolome and gut microbiota in an aging Duroc boars model.
CAAS - Chinese Academy of Agricultural Sciences [CN]
Funding text :
The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This research was supported by the funding of CAAS of Agricultural Science and Technology Innovation Program (CAAS-ZDRW202006-02, ASTIPIAS07) and the State Key Laboratory of Animal Nutrition (2004DA125184G2102). Acknowledgments
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