Effect of dexamethasone on gene expression of cannabinoid receptor type 1 and adenosine monophosphate-activated protein kinase in the hypothalamus of broilers (Gallus domesticus).
Kong, Linglian; Xiao, Chuanpi; Lin, Haiet al.
2021 • In Comparative Biochemistry and Physiology. Part A, Molecular and Integrative Physiology, 260, p. 111018
[en] Hypothalamic neural circuits play a critical role in integrating peripheral signals and conveying information about energy and nutrient status. We detected cannabinoid receptor type 1 (CB1) distribution in the hypothalamus, liver, duodenum, jejunum, and ileum among 7- and 35-day-old broilers. The effects of dexamethasone (DEX) on CB1 gene expression were evaluated by in vitro and in vivo experiments on glucocorticoid receptor (GR) and adenosine monophosphate-activated protein kinase (AMPK) in the hypothalamus of broilers. In vitro, hypothalamic cells from 17-day-old broiler embryos were incubated with either 0.1% dimethyl sulfoxide or DEX (100 nmol/mL) for 1 h. In the in vivo study, 28-day-old broilers were injected with DEX for 24 h or 72 h. Results showed that CB1 was mainly expressed in the hypothalamus, and 72 h DEX treatment increased the expression. One-day treatment of broilers with DEX did not change the hypothalamic CB1 gene expression. Moreover, DEX treatment for 24 h and 72 h increased the mRNA level of hypothalamic AMPKα2 and GR. However, no differences were observed on the gene expression of CB1, GR, and AMPKα2 in hypothalamic cells with DEX-treated for 1 h. In conclusion, CB1 is mainly expressed in the hypothalamus of broilers; 72-h DEX exposure can regulate the CB1 system and AMPK signaling pathway of the broiler hypothalamus.
Kong, Linglian; Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271018, China
Xiao, Chuanpi ; Université de Liège - ULiège > TERRA Research Centre
Lin, Hai; Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271018, China
Buyse, Johan; Division Animal and Human Health Engineering, Department of Biosystems, KU Leuven, Kasteelpark Arenberg 30, Leuven 3001, Belgium
Li, Xianlei; Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271018, China
Song, Zhigang; Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271018, China. Electronic address: zhigangs@sdau.edu.cn
Language :
English
Title :
Effect of dexamethasone on gene expression of cannabinoid receptor type 1 and adenosine monophosphate-activated protein kinase in the hypothalamus of broilers (Gallus domesticus).
Publication date :
October 2021
Journal title :
Comparative Biochemistry and Physiology. Part A, Molecular and Integrative Physiology
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