[en] This study aimed to investigate the effects of diets supplemented with 25-hydroxycholecalciferol [25-(OH)D3] and additional vitamin E on growth performance, antioxidant capacity, bone development, and carcass characteristics at different stocking densities on commercial broiler farms. A total of 118,800 one-day-old Arbor Acres broilers were assigned to a 2 × 2 factorial treatment consisting of two dietary vitamin levels (5,500 IU vitamin D3 and 60 IU vitamin E: normal diet, using half 25-(OH)D3 as a source of vitamin D3 and an additional 60 IU of vitamin E: 25-(OH)D3+VE diet) and two stocking densities (high density of 20 chickens/m2: HD and 16 chickens/m2: LD). The experiment lasted for 42 d. The results showed that high-density stocking negatively affected the growth performance of broilers during the first four weeks, whereas the vitamin diet treatment significantly improved the feed conversion ratios (FCR) during the last 2 wk. Vitamin diets increased catalase at 14 and 42 d, and the glutathione peroxidase (GSH-px) levels at 42 d in high-density-stocked broilers. The interaction showed that serum vitamin E levels were significantly improved at 28 d of age in high-density-stocked broilers as a result of the vitamin diets. Stocking density and dietary treatments were found to significantly affect bone development, with the vitamin diet significantly increasing metatarsal length and femoral bone strength in broilers from high-density stocking density at 28 d of age. High stocking density increased the proportion of leg muscles and meat yield per square meter. In general, 25-(OH)D3 and additional vitamin E suppressed oxidative stress and ameliorated the negative effects of high-density stocking on bone development in a commercial chicken farm setting. Vitamin diets improved the FCR of broilers, while high-density stocking resulted in better economic outcomes. [en] High-density stocking is often associated with animal welfare risks in broilers, mainly in terms of oxidative stress and bone development. Nevertheless, farming at too low a density remains for the most part economically unviable. Modulation of antioxidant capacity and bone development by nutritional strategies in high-density-farmed broilers has proven an effective tool in developing countries. Therefore, the present study investigated the effects of applying diets with a higher biological potency of vitamin D3 25-hydroxycholecalciferol [25-(OH)D3] and a higher concentration of vitamin E on broiler production performance, antioxidant capacity and meat production performance at different densities of stocking under commercial farming conditions. The results indicated that the vitamin dietary treatments suppressed oxidative stress and ameliorated the negative effects of high-density farming on bone development.
Disciplines :
Animal production & animal husbandry
Author, co-author :
Xiao, Chuanpi ; Université de Liège - ULiège > TERRA Research Centre ; Key Laboratory of Efficient Utilization of Non-grain Feed Resources, Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271000. China
Zhu, Qijiang; Key Laboratory of Efficient Utilization of Non-grain Feed Resources, Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271000. China
Comer, Luke; Nutrition and Animal Microbiota Ecosystems lab, Department of Biosystems, KU Leuven, Leuven 3000, Belgium
Pan, Xue; Key Laboratory of Efficient Utilization of Non-grain Feed Resources, Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271000. China
Everaert, Nadia ; Université de Liège - ULiège > TERRA Research Centre > Animal Sciences (AS) ; Key Laboratory of Efficient Utilization of Non-grain Feed Resources, Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271000. China
Schroyen, Martine ; Université de Liège - ULiège > Département GxABT > Animal Sciences (AS)
Song, Bochen; Key Laboratory of Efficient Utilization of Non-grain Feed Resources, Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271000. China
Song, Zhigang; Key Laboratory of Efficient Utilization of Non-grain Feed Resources, Department of Animal Science, Shandong Agricultural University, Taian, Shandong 271000. China
Language :
English
Title :
Dietary 25-hydroxy-cholecalciferol and additional vitamin E improve bone development and antioxidant capacity in high-density stocking broilers.
Abudabos, A. M., E. M. Samara, E. O. Hussein, M. Q. Al-Ghadi, and R. M. Al-Atiyat. 2013. Impacts of stocking density on the performance and welfare of broiler chickens. Ital. J. Anim. Sci. 12:e11. doi:10.5455/javar.2021.h556
Al-Baadani, H. H., R. A. Alhotan, and M. M. Azzam. 2023. Evaluation of the interaction between gum Arabic addition and stocking density on growth performance, carcass characteristics, and general health parameters of broiler chickens. Animals (Basel) 13:3024. doi:10.3390/ani13193024
Atencio, A., H. Edwards, Jr, and G. Pesti. 2005. Effects of vitamin D3 dietary supplementation of broiler breeder hens on the performance and bone abnormalities of the progeny. Poult. Sci. 84:1058–1068. doi:10.1093/ps/84.7.1058
Bortoluzzi, C., B. S. Vieira, and T. J. Applegate. 2020. Influence of dietary zinc, copper, and manganese on the intestinal health of broilers under Eimeria challenge. Front. Vet. Sci. 7:13. doi:10.3389/fvets.2020.00013
Buijs, S., E. Van Poucke, S. Van Dongen, L. Lens, J. Baert, and F. A. Tuyttens. 2012. The influence of stocking density on broiler chicken bone quality and fluctuating asymmetry. Poult. Sci. 91:1759–1767. doi:10.3382/ps.2011-01859
Chou, S., T. Chung, and B. Yu. 2009. Effects of supplemental 25-hy-droxycholecalciferol on growth performance, small intestinal morphology, and immune response of broiler chickens. Poult. Sci. 88:2333–2341. doi:10.3382/ps.2009-00283
Dozier, W., J. Thaxton, S. Branton, G. Morgan, D. Miles, W. Roush, B. Lott, and Y. Vizzier-Thaxton. 2005. Stocking density effects on growth performance and processing yields of heavy broilers. Poult. Sci. 84:1332–1338. doi:10.1093/ps/84.8.1332
Feddes, J. J., E. J. Emmanuel, and M. J. Zuidhoft. 2002. Broiler performance, body weight variance, feed and water intake, and carcass quality at different stocking densities. Poult. Sci. 81:774–779. doi:10.1093/ps/81.6.774
Fritts, C., and P. Waldroup. 2003. Effect of source and level of vitamin D on live performance and bone development in growing broilers. J. Appl. Poult. Res. 12:45–52. doi:10.1093/japr/12.1.45
Garcia, A. F. Q. M., A. E. Murakami, C. R. do Amaral Duarte, I. C. O. Rojas, K. P. Picoli, and M. M. Puzotti. 2013. Use of vitamin D 3 and its metabolites in broiler chicken feed on performance, bone parameters and meat quality. Asian-Australas. J. Anim. Sci. 26:408–415. doi:10.5713/ajas.2012.12455
Han, J., L. Wu, X. Lv, M. Liu, Y. Zhang, L. He, J. Hao, L. Xi, H. Qu, and C. Shi. 2022. Intestinal segment and vitamin D3 concentration affect gene expression levels of calcium and phosphorus transporters in broiler chickens. J. Anim. Sci. Technol 65:336–350. doi:10.5187/ jast.2022.e78
Hashem, N., A. Abd El-Hady, and O. Hassan. 2017. Inclusion of phytogenic feed additives comparable to vitamin E in diet of growing rabbits: Effects on metabolism and growth. Ann. Agric. Sci. 62:161–167. doi:10.1016/j.aoas.2017.11.003
Hester, P., M. Schreiweis, J. Orban, H. Mazzuco, M. Kopka, M. Ledur, and D. Moody. 2004. Assessing bone mineral density in vivo: dual energy X-ray absorptiometry. Poult. Sci. 83:215–221. doi:10.1093/ ps/83.2.215
Hsu, S., L. R. Zelnick, Y. S. Lin, C. M. Best, B. Kestenbaum, K. E. Thummel, L. M. Rose, A. N. Hoofnagle, and I. H. de Boer. 2021. Differences in 25-hydroxyvitamin D clearance by eGFR and race: a pharmacokinetic study. J. Am. Soc. Nephrol. 32:188–198. doi:10.1681/ASN.2020050625
Incharoen, T., S. Roytrakul, and W. Likittrakulwong. 2021. Dietary germinated paddy rice and stocking density affect egg performance, serum biochemical properties, and proteomic and transcriptomic response of laying hens exposed to chronic heat stress. Proteomes 9:48. doi:10.3390/proteomes9040048
Jiang, S., Z. Jiang, K. Yang, F. Chen, C. Zheng, and L. Wang. 2015. Dietary vitamin D3 requirement of Chinese yellow-feathered broilers. Poult. Sci. 94:2210–2220. doi:10.3382/ps/pev163
Karaarslan, S., and A. Nazlıgül. 2018. Effects of lighting, stocking density, and access to perches on leg health variables as welfare indicators in broiler chickens. Livest. Sci. 218:31–36. doi:10.1016/j. livsci.2018.10.008
Kong, L., Z. Wang, C. Xiao, Q. Zhu, and Z. Song. 2022b. Glycerol monolaurate attenuated immunological stress and intestinal mucosal injury by regulating the gut microbiota and activating AMPK/Nrf2 signaling pathway in lipopolysaccharide-challenged broilers. Anim. Nutr. 10:347–359. doi:10.1016/j. aninu.2022.06.005
Kumar, P., M. A. Ahmed, A. A. Abubakar, M. N. Hayat, U. Kaka, M. Ajat, G. Y. Meng, and A. Q. Sazili. 2022. Improving animal welfare status and meat quality through assessment of stress bio-markers: a critical review. Meat Sci. 197:109048. doi:10.1016/j. meatsci.2022.109048
Lallo, C. H., M. Williams, M. Campbell, and D. W. Palmer. 2012. The effect of stocking density on the performance and economic implications for broilers grown to 42 days in open sided house in Trinidad. Trop. Agric. (Trinidad). 89:170. https://journals.sta.uwi.edu/ojs/index.php/ta/article/view/989
Liem, A. 2009. Dietary factors influencing calcium and phosphorus utilization by broiler chicks. Doctoral Dissertation, University of Georgia, Athens.
Lin, H., E. Decuypere, and J. Buyse. 2006. Acute heat stress induces oxidative stress in broiler chickens. Comp. Biochem. Physiol. A: Mol. Integr. Physiol. 144:11–17. doi:10.1016/j.cbpa.2006.01.032
Luo, J., Z. Zhou, H. Zhang, R. Ma, and J. Hou. 2013. Bone response of broiler chickens (Gallus gallus domesticus) induced by corticosterone. Comp. Biochem. Physiol. A: Mol. Integr. Physiol. 164:410–416. doi:10.1016/j.cbpa.2012.12.001
Ma, H., B. Xu, W. Li, F. Wei, W. K. Kim, C. Chen, Q. Sun, C. Fu, G. Wang, and S. Li. 2020. Effects of alpha-lipoic acid on the behavior, serum indicators, and bone quality of broilers under stocking density stress. Poult. Sci. 99:4653–4661. doi:10.1016/j.psj.2020.05.007
Magnuson, A. D., G. Liu, T. Sun, S. A. Tolba, L. Xi, R. Whelan, and X. G. Lei. 2020. Supplemental methionine and stocking density affect antioxidant status, fatty acid profiles, and growth performance of broiler chickens. J. Anim. Sci. 98:skaa092. doi:10.1093/ jas/skaa092
Marchewka, J., P. Sztandarski, M. Solka, H. Louton, K. Rath, L. Vogt, E. Rauch, D. Ruijter, I. C. de Jong, and J. O. Horbańczuk. 2022. Linking key husbandry factors to the intrinsic quality of broiler meat. Poult. Sci. 102:102384. doi:10.1016/j.psj.2022.102384
Mazur-Kuśnirek, M., Z. Antoszkiewicz, K. Lipiński, J. Kaliniewicz, S. Kotlarczyk, and P. Żukowski. 2019. The effect of polyphenols and vitamin E on the antioxidant status and meat quality of broiler chickens exposed to high temperature. Arch. Anim. Nutr. 73:111–126. doi:10.1080/1745039X.2019.1572342
Mishra, B., and R. Jha. 2019. Oxidative stress in the poultry gut: potential challenges and interventions. Front. Vet. Sci 6:60. doi:10.3389/ fvets.2019.00060
Nasr, M. A., A. Q. Alkhedaide, A. A. Ramadan, E. H. Abd-El Salam, and M. A. Hussein. 2021. Potential impact of stocking density on growth, carcass traits, indicators of biochemical and oxidative stress and meat quality of different broiler breeds. Poult. Sci. 100:101442. doi:10.1016/j.psj.2021.101442
Niu, Z., F. Liu, Q. Yan, and W. Li. 2009. Effects of different levels of vitamin E on growth performance and immune responses of broilers under heat stress. Poult. Sci. 88:2101–2107. doi:10.3382/ ps.2009-00220
Onbaşılar, E. E., M. Kahraman, F. Güngör, A. Kocakaya, T. Karakan, M. Pirpanahi, B. Doğan, D. Metin, M. Akan, A. Şehu, et al. 2020. Effects of cage type on performance, welfare, and microbiological properties of laying hens during the molting period and the second production cycle. Trop. Anim. Health Prod. 52:3713–3724. doi:10.1007/s11250-020-02409-0
Ong, J. S., S. C. Dixon-Suen, X. Han, J. An, U. Liyanage, J. C. Dusingize, J. Schumacher, I. Gockel, A. Böhmer, J. Jankowski, et al.; Esophageal Cancer Consortium. 2021. A comprehensive re-assessment of the association between vitamin D and cancer susceptibility using Mendelian randomization. Nat. Commun. 12:246. doi:10.1038/ s41467-020-20368-w
Onyango, E., P. Hester, R. Stroshine, and O. Adeola. 2003. Bone den-sitometry as an indicator of percentage tibia ash in broiler chicks fed varying dietary calcium and phosphorus levels. Poult. Sci. 82:1787–1791. doi:10.1093/ps/82.11.1787
Ostadmohammadi, V., M. Jamilian, F. Bahmani, and Z. Asemi. 2019. Vitamin D and probiotic co-supplementation affects mental health, hormonal, inflammatory and oxidative stress parameters in women with polycystic ovary syndrome. J. Ovarian. Res. 12:5. doi:10.1186/ s13048-019-0480-x
Oviedo-Rondón, E. O. 2022. Optimizing the health of broilers. North Carolina State University.
Panda, A. K., and G. Cherian. 2014. Role of vitamin E in counteracting oxidative stress in poultry. Poult. Sci. 51:109–117. doi:10.2141/ jpsa.0130134
Pitargue, F. M., J. H. Kim, D. Goo, J. B. Delos Reyes, and D. Y. Kil. 2019. Effect of vitamin E sources and inclusion levels in diets on growth performance, meat quality, alpha-tocopherol retention, and intestinal inflammatory cytokine expression in broiler chickens. Poult. Sci. 98:4584–4594. doi:10.3382/ps/pez149
Rahal, A., A. Kumar, V. Singh, B. Yadav, R. Tiwari, S. Chakraborty, and K. Dhama. 2014. Oxidative stress, prooxidants, and antioxidants: the interplay. Biomed Res. Int. 2014:761264. doi:10.1155/2014/761264
Rey, A. I., J. F. Segura, D. Castejón, E. Fernández-Valle, M. I. Cambero, and L. Calvo. 2020. Vitamin D3 supplementation in drinking water prior to slaughter improves oxidative status, physiological stress, and quality of pork. Antioxidants (Basel) 9:559. doi:10.3390/antiox9060559
Sahin, K., N. Sahin, M. Onderci, S. Yaralioglu, and O. Kucuk. 2001. Protective role of supplemental vitamin E on lipid peroxidation, vitamins E, A and some mineral concentrations of broilers reared under heat stress. Vet Med 46:140–144. doi:10.17221/7870-vetmed
Sandøe, P., H. O. Hansen, B. Forkman, P. van Horne, H. Houe, I. C. de Jong, J. B. Kjær, S. S. Nielsen, C. Palmer, and H. L. H. Rhode. 2022. Market driven initiatives can improve broiler welfare – a comparison across five European countries based on the Benchmark method. Poult. Sci. 101:101806. doi:10.1016/j. psj.2022.101806
Shao, Y., G. Sun, S. Cao, L. Lu, L. Zhang, X. Liao, and X. Luo. 2019. Bone phosphorus retention and bone development of broilers at different ages. Poult. Sci. 98:2114–2121. doi:10.3382/ps/pey565
Shehata, S. F., S. H. Baloza, M. M. M. Elsokary, N. M. Hashem, and M. M. Khawanda. 2022. Effect of stocking density and vitamin E or zinc supplementation on growth, physiology, gene expression, and economic efficiency of growing broiler chicks. Trop. Anim. Health Prod. 54:403. doi:10.1007/s11250-022-03382-6
Simitzis, P., E. Kalogeraki, M. Goliomytis, M. Charismiadou, K. Triantaphyllopoulos, A. Ayoutanti, K. Niforou, A. Hager-Theodorides, and S. Deligeorgis. 2012. Impact of stocking density on broiler growth performance, meat characteristics, behavioural components and indicators of physiological and oxidative stress. Br. Poult. Sci. 53:721–730. doi:10.1080/00071668.2012.745930
Stamp Dawkins, M., C. A. Donnelly, and T. A. Jones. 2004. Chicken welfare is influenced more by housing conditions than by stocking density. Nature 427:342–344. doi:10.1038/nature02226
Sun, Z., Q. Fan, X. Wang, Y. Guo, H. Wang, and X. Dong. 2018. High stocking density alters bone-related calcium and phosphorus metabolism by changing intestinal absorption in broiler chickens. Poult. Sci. 97:219–226. doi:10.3382/ps/pex294
Surai, P. F., I. I. Kochish, M. N. Romanov, and D. K. Griffin. 2019. Nutritional modulation of the antioxidant capacities in poultry: the case of vitamin E. Poult. Sci. 98:4030–4041. doi:10.3382/ps/pez072
Wang, J., C. Zhang, T. Zhang, L. Yan, L. Qiu, H. Yin, X. Ding, S. Bai, Q. Zeng, X. Mao, et al. 2021a. Dietary 25-hydroxyvitamin D improves intestinal health and microbiota of laying hens under high stocking density. Poult. Sci. 100:101132. doi:10.1016/j.psj.2021.101132
Wang, Y. Q., X. P. Geng, M. W. Wang, H. Q. Wang, C. Zhang, X. He, S. M. Liang, D. X. Xu, and X. Chen. 2021b. Vitamin D deficiency exacerbates hepatic oxidative stress and inflammation during acetaminophen-induced acute liver injury in mice. Int. Immunopharmacol. 97:107716. doi:10.1016/j.intimp.2021.107716
Wang, L., L. Kong, X. Hu, H. Bai, Z. Wang, Y. Jiang, Y. Bi, G. Chang, and G. Chen. 2022. Effect of stocking density on performance, meat quality and cecal bacterial communities of yellow feather broilers. Anim. Biotechnol. 33:1322–1332. doi:10.1080/1049539 8.2021.1898413
Wang, X., X. Liu, S. Liu, J. Qu, M. Ye, J. Wang, X. Li, Z. Yuan, J. Wu, J. Yi, et al. 2023. Effects of anti-stress agents on the growth performance and immune function in broiler chickens with vaccination-induced stress. Avian Pathol. 52:12–24. doi:10.1080/03079 457.2022.2114874
Wideman Jr, R., J. Blankenship, I. Pevzner, and B. Turner. 2015. Efficacy of 25-OH vitamin D3 prophylactic administration for reducing lameness in broilers grown on wire flooring. Poult. Sci. 94:1821–1827. doi:10.3382/ps/pev160
Zdanowska-Sąsiadek, Z., M. Michalczuk, K. Damaziak, J. Niemiec, E. Poławska, D. Gozdowski, and E. Różańska. 2016. Effect of vitamin E supplementation on growth performance and chicken meat quality. Europ. Poult. Sci. 80:1–14. doi:10.3382/ps/pez149
