[en] The integrity of the intestinal barrier is critical for homeostasis. In this study, we investigated the protective effect of pterostilbene (PTE) on the intestinal epithelium barrier. In vitro results of transepithelial electrical resistance (TEER) in Caco-2 cells indicated that PTE counteracted tumor necrosis factor α (TNFα)-induced barrier damage. In vivo PTE pretreatment markedly ameliorated intestinal barrier dysfunction induced by dextran sulfate sodium (DSS). Notably, intestinal epithelial tight junction (TJ) molecules were restored by PTE in mice exposed to DSS. The mechanism study revealed that PTE prevented myosin light-chain kinase (MLCK) from driving phosphorylation of MLC (p-MLC), which is crucial for maintaining intestinal TJ stability. Furthermore, PTE blunted translocation of NF-κB subunit p65 into the nucleus to downregulate MLCK expression and then to safeguard TJs and barrier integrity. These findings suggest that PTE protected the intestinal epithelial barrier through the NF-κB- MLCK/p-MLC signal pathway.
Disciplines :
Gastroenterology & hepatology
Author, co-author :
Wang, Juan; Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China
Zhao, Hui ; Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China
Lv, Ke; Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China ; Hubei Key Laboratory of EFGIR, Huanggang Normal University, Huanggang, Hubei 438000, China
Zhao, Wei; Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China
Zhang, Ning; Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China
Yang, Fan; Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China
Wen, Xiang ; Université de Liège - ULiège > GIGA > GIGA Stem Cells - Medical Chemistry ; Tianjin Key Laboratory of Food and Biotechnology, School of Biotechnology and Food Science, Tianjin University of Commerce, Tianjin 300134, China
Jiang, Xiaohua; Department of Histololgy and Embrylolgy, School of Basic Medicine, North China University of Science and Technology, 21 Bohai Road, Caofeidian Xincheng, Tangshan, Hebei 063210, China
Tian, Jingrui; Department of Histololgy and Embrylolgy, School of Basic Medicine, North China University of Science and Technology, 21 Bohai Road, Caofeidian Xincheng, Tangshan, Hebei 063210, China
Liu, Xinjuan; Department of Gastroenterology, Beijing Chaoyang Hospital, Capital Medical University, Chaoyang District, Beijing100024, China
Ho, Chi-Tang ; Department of Food Science, Rutgers University, New Brunswick, New Jersey 08901, United States
Li, Shiming; Hubei Key Laboratory of EFGIR, Huanggang Normal University, Huanggang, Hubei 438000, China ; Department of Food Science, Rutgers University, New Brunswick, New Jersey 08901, United States
Language :
English
Title :
Pterostilbene Ameliorates DSS-Induced Intestinal Epithelial Barrier Loss in Mice via Suppression of the NF-κB-Mediated MLCK-MLC Signaling Pathway.
NSCF - National Natural Science Foundation of China [CN]
Funding text :
This work was supported by grants from the National Natural Science Foundation of China (82070559), the Tianjin Innovative Team Project (TD13-5087), the Tianjin Natural Science Foundation (19JCQNJC12400), the Shangrao Crucial Research and Development Project (19A005), and the Grant from Hubei Province, China (GRANT number 2019ABA100).
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