[en] Senecavirus A (SVA) is the pathogen that has recently caused porcine idiopathic vesicular disease (PIVD). The clinical symptoms of PIVD are similar to those of acute foot-and-mouth disease and also can result in the death of newborn piglets, thus entailing economic losses. Vaccine immunization is the most effective way to prevent and control SVA. Among all SVA vaccines reported, only the SVA inactivated vaccine has been successfully developed. However, to ensure the elimination of this pathogen, safer and more effective vaccines are urgently required. A virus-like particles (VLPs)-based vaccine is probably the best alternative to inactivated vaccine. To develop an SVA VLPs vaccine and evaluate its immune effect, a prokaryotic expression system was used to produce SVA capsid protein and assemble VLPs. The VLPs were characterized by affinity chromatography, sucrose density gradient centrifugation, ZetaSizer and transmission electron microscopy. Meanwhile, the SVA CH-HB-2017 strain was used to infect pigs and to determine infection routes and dose. Experimental pigs were then immunized with the SVA VLPs vaccine emulsified in an ISA 201 adjuvant. The results showed that the VLPs vaccine induced neutralizing and specific antibodies at similar levels as an inactivated SVA vaccine after immunization. The level of INF-γ induced by the VLPs vaccine gradually decreased-similar to that of inactivated vaccine. These results indicated that VLPs vaccine may simultaneously cause both cellular and humoral immune responses. Importantly, after the challenge, the VLPs vaccine provided similar levels of protection as the inactivated SVA vaccine. In this study, we successfully obtained novel SVA VLPs and confirmed their highly immunogenicity, thus providing a superior candidate vaccine for defense and elimination of SVA, compared to the inactivated vaccine.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Mu, Suyu; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
Sun, Shiqi; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
Dong, Hu; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
Bai, Manyuan; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
Zhang, Yun; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
Teng, Zhidong; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
Ren, Mei ; Université de Liège - ULiège > TERRA Research Centre ; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
Yin, Shuanghui; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
Guo, Huichen; Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China ; College of Animal Science, Yangtze University, Jingzhou 434025, China
Language :
English
Title :
Potent Protective Immune Responses to Senecavirus Induced by Virus-Like Particle Vaccine in Pigs.
NSCF - National Natural Science Foundation of China
Funding text :
Funding: This work was supported by grants from the National Key Research and Development Program of China (2017YFD0500900, 2017YFD0501100, 2016YFE0204100), Central Public-interest Scientific Institution Basal Research Fund (1610312018003, 1610312016002), the National Natural Science Foundation of China (31672592, 31873023, 31811540395), Elite Youth program of Chinese Academy of Agricultural Sciences.This work was supported by grants from the National Key Research and Development Program of China (2017YFD0500900, 2017YFD0501100, 2016YFE0204100), Central Public-interest Scientific Institution Basal Research Fund (1610312018003, 1610312016002), the National Natural Science Foundation of China (31672592, 31873023, 31811540395), Elite Youth program of Chinese Academy of Agricultural Sciences. Acknowledgments: Thanks for the support of Lanzhou Veterinary Research Institute in the animal house to facilitate the active development of animal experiment.
Wu, Q.; Zhao, X.; Chen, Y.; He, X.; Zhang, G.; Ma, J. Complete Genome Sequence of Seneca Valley Virus CH-01-2015 Identified in China. Genome Announc. 2016, 4, e01509-15. [CrossRef]
Saeng-Chuto, K.; Rodtian, P.; Temeeyasen, G.; Wegner, M.; Nilubol, D. The first detection of Senecavirus A in pigs in Thailand, 2016. Transbound. Emerg. Dis. 2018, 65, 285–288. [CrossRef] [PubMed]
Sun, D.; Vannucci, F.; Knutson, T.P.; Corzo, C.; Marthaler, D.G. Emergence and whole-genome sequence of Senecavirus A in Colombia. Transbound. Emerg. Dis. 2017, 64, 1346–1349. [CrossRef] [PubMed]
Arzt, J.; Bertram, M.R.; Vu, L.T.; Pauszek, S.J.; Hartwig, E.J.; Smoliga, G.R.; Palinski, R.; Stenfeldt, C.; Fish, I.H.; Hoang, B.H.; et al. First Detection and Genome Sequence of Senecavirus A in Vietnam. Microbiol. Resour. Announc. 2019, 8, e01247-18. [CrossRef]
Segales, J.; Barcellos, D.; Alfieri, A.; Burrough, E.; Marthaler, D. Senecavirus A: An Emerging Pathogen Causing Vesicular Disease and Mortality in Pigs? Vet. Pathol. 2017, 54, 11–21. [CrossRef]
Qian, S.; Fan, W.; Liu, T.; Wu, M.; Zhang, H.; Cui, X.; Zhou, Y.; Hu, J.; Wei, S.; Chen, H.; et al. Seneca Valley Virus Suppresses Host Type I Interferon Production by Targeting Adaptor Proteins MAVS, TRIF, and TANK for Cleavage. J. Virol. 2017, 91. [CrossRef]
Paavonen, J.; Jenkins, D.; Bosch, F.X.; Naud, P.; Salmeron, J.; Wheeler, C.M.; Chow, S.N.; Apter, D.L.; Kitchener, H.C.; Castellsague, X.; et al. Efficacy of a prophylactic adjuvanted bivalent L1 virus-like-particle vaccine against infection with human papillomavirus types 16 and 18 in young women: An interim analysis of a phase III double-blind, randomised controlled trial. Lancet 2007, 369, 2161–2170. [CrossRef]
Liu, H.; Ma, Y.; Wang, H.; Liu, Q. Quantitative evaluation of the effect of the hepatitis B vaccine based on the HBsAg-and anti-HBs-positive rates in the Chinese population over the last 33 years. Vaccine 2012, 30, 3483–3487. [CrossRef] [PubMed]
Michel, M.L.; Tiollais, P. Hepatitis B vaccines: Protective efficacy and therapeutic potential. Pathol. Biol. (Paris) 2010, 58, 288–295. [CrossRef]
Noad, R.; Roy, P. Virus-like particles as immunogens. Trends. Microbiol. 2003, 11, 438–444. [CrossRef]
Yin, S.; Sun, S.; Yang, S.; Shang, Y.; Cai, X.; Liu, X. Self-assembly of virus-like particles of porcine circovirus type 2 capsid protein expressed from Escherichia coli. Virol. J. 2010, 7, 166. [CrossRef]
Guo, H.C.; Sun, S.Q.; Jin, Y.; Yang, S.L.; Wei, Y.Q.; Sun, D.H.; Yin, S.H.; Ma, J.W.; Liu, Z.X.; Guo, J.H.; et al. Foot-and-mouth disease virus-like particles produced by a SUMO fusion protein system in Escherichia coli induce potent protective immune responses in guinea pigs, swine and cattle. Vet. Res. 2013, 44, 48. [CrossRef] [PubMed]
Guo, H.; Zhu, J.; Tan, Y.; Li, C.; Chen, Z.; Sun, S.; Liu, G. Self-assembly of virus-like particles of rabbit hemorrhagic disease virus capsid protein expressed in Escherichia coli and their immunogenicity in rabbits. Antivir. Res. 2016, 131, 85–91. [CrossRef] [PubMed]
Xiao, Y.; Chen, H.Y.; Wang, Y.; Yin, B.; Lv, C.; Mo, X.; Yan, H.; Xuan, Y.; Huang, Y.; Pang, W.; et al. Large-scale production of foot-and-mouth disease virus (serotype Asia1) VLP vaccine in Escherichia coli and protection potency evaluation in cattle. BMC Biotechnol. 2016, 16, 56. [CrossRef] [PubMed]
Strauss, M.; Jayawardena, N.; Sun, E.; Easingwood, R.; Burga, L.N.; Bostina, M. Cryo-EM Structure of Seneca Valley Virus Procapsid. J. Virol. 2017, 92, e01927-17. [CrossRef]
Venkataraman, S.; Reddy, S.P.; Loo, J.; Idamakanti, N.; Hallenbeck, P.L.; Reddy, V.S. Crystallization and preliminary X-ray diffraction studies of Seneca Valley virus-001, a new member of the Picornaviridae family. Acta Crystallogr. Sect. F Struct. Biol. Cryst. Commun. 2008, 64, 293–296. [CrossRef]
Hales, L.M.; Knowles, N.J.; Reddy, P.S.; Xu, L.; Hay, C.; Hallenbeck, P.L. Complete genome sequence analysis of Seneca Valley virus-001, a novel oncolytic picornavirus. J. Gen. Virol. 2008, 89, 1265–1275. [CrossRef]
Maggioli, M.F.; Lawson, S.; de Lima, M.; Joshi, L.R.; Faccin, T.C.; Bauermann, F.V.; Diel, D.G. Adaptive Immune Responses following Senecavirus A Infection in Pigs. J. Virol. 2018, 92, e01717-17. [CrossRef]
Sadr, V.; Saffar, B.; Emamzadeh, R. Functional expression and purification of recombinant Hepcidin25 production in Escherichia coli using SUMO fusion technology. Gene 2017, 610, 112–117. [CrossRef]
Heinrich, J.; Drewniok, C.; Neugebauer, E.; Kellner, H.; Wiegert, T. The YoaW signal peptide directs efficient secretion of different heterologous proteins fused to a StrepII-SUMO tag in Bacillus subtilis. Microb. Cell Fact. 2019, 18, 31. [CrossRef]
Marblestone, J.G.; Edavettal, S.C.; Lim, Y.; Lim, P.; Zuo, X.; Butt, T.R. Comparison of SUMO fusion technology with traditional gene fusion systems: Enhanced expression and solubility with SUMO. Protein. Sci. 2006, 15, 182–189. [CrossRef] [PubMed]
Maggioli, M.F.; Fernandes, M.H.V.; Joshi, L.R.; Sharma, B.; Tweet, M.M.; Noll, J.C.G.; Bauermann, F.V.; Diel, D.G. Persistent infection and transmission of Senecavirus A from carrier sows to contact piglets. J. Virol. 2019, 93, e00819-19. [CrossRef] [PubMed]
Joshi, L.R.; Fernandes, M.H.; Clement, T.; Lawson, S.; Pillatzki, A.; Resende, T.P.; Vannucci, F.A.; Kutish, G.F.; Nelson, E.A.; Diel, D.G. Pathogenesis of Senecavirus A infection in finishing pigs. J. Gen. Virol. 2016, 97, 3267–3279. [CrossRef]
Xiaoran, G.; Shichong, H.; Xiaoli, Z.; Shiqi, S.; Xiaojun, M.; Huichen, G. Isolation, Identification and Biological Characteristics of the Chinese Strain of Senecavirus A. Chin. J. Virol. 2018, 34, 555–562.
Oliveira, A.N.; Andrade, M.C.; Silva, M.V.; Moura, W.C.; Cortez Contreiras, E. Immune response in cattle vaccinated against rabies. Mem Inst. Oswaldo. Cruz. 2000, 95, 83–88. [CrossRef] [PubMed]
Scodeller, E.A.; Lebendiker, M.A.; Dubra, M.S.; Crespo, O.A.; Basarab, O.; La Torre, J.L.; Vasquez, C. Inactivation of foot-and-mouth disease virus vaccine strains by activation of virus-associated endonuclease. J. Gen. Virol. 1984, 65, 1567–1573. [CrossRef] [PubMed]
Mu, S.; Abdullah, S.W.; Zhang, Y.; Han, S.; Guo, H.; Li, M.; Dong, H.; Xu, J.; Teng, Z.; Wen, X.; et al. Development of a novel SYBR green I-based quantitative RT-PCR assay for Senecavirus A detection in clinical samples of pigs. Mol. Cell Probes 2020, 53, 101643. [CrossRef] [PubMed]
