[en] [en] BACKGROUND: Yak, a predominant livestock of plateau areas, is known as a host to many parasites. And the genus Entamoeba, the third-common cause of the mortality worldwide from parasitic diseases, was discovered in yaks once.
METHODS: We investigated the distribution and species of Entamoeba spp. from yaks in Qinghai province, northwestern China, by collecting 1027 yak fecal samples. All samples were divided according to seven geographical sites, four seasons, and two age groups of yaks. After extracting DNA, polymerase chain reaction (PCR) was performed to amplify the 18S rRNA gene, and sequences were analyzed with phylogenetic method.
RESULTS: We observed an overall Entamoeba positive rate of 36.32% (373/1027) in yaks from Qinghai province. The common species included Entamoeba bovis (284/373), Entamoeba sp. MG107/BEL (79/373), Entamoeba sp. ribosomal lineage (RL) two (8/373), and Entamoeba sp. RL9 (2/373). According to the result of statistical analysis, Entamoeba infection rate was the highest in summer and significantly differed from that observed during other seasons (P < 0.05). The yaks from Golog had the highest prevalence of Entamoeba among all geographical origins in Qinghai province (P < 0.05). However, no significant difference was observed (P > 0.05) among different age groups, as evident from a positive rate of 39.58% in ≤ 6-month and 36.16% in > 6-month yaks.
CONCLUSION: These results indicate the prevalence and predominant species of Entamoeba in yaks. To our knowledge, this is the first study to report E. bovis, Entamoeba sp. RL2, and Entamoeba sp. RL9 in Chinese yaks.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Ren, Mei ; Université de Liège - ULiège > TERRA Research Centre ; College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi Province, People's Republic of China ; State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, 810016, Qinghai Province, People's Republic of China ; State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu Province, People's Republic of China
Yang, Fan; College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi Province, People's Republic of China
Gou, Jing-Min; College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi Province, People's Republic of China
Wang, Pin-Xue; College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi Province, People's Republic of China
Zou, Min; College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi Province, People's Republic of China
Zhong, Xiao-Hong; College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi Province, People's Republic of China
Lin, Qing ; College of Veterinary Medicine, Northwest A&F University, Yangling, 712100, Shaanxi Province, People's Republic of China. yllinqing@126.com ; State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, 810016, Qinghai Province, People's Republic of China. yllinqing@126.com ; State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, 730046, Gansu Province, People's Republic of China. yllinqing@126.com
Language :
English
Title :
First Detection and Molecular Identification of Entamoeba in Yaks from China.
Publication date :
March 2021
Journal title :
Acta Parasitologica
ISSN :
1230-2821
eISSN :
1896-1851
Publisher :
Springer Science and Business Media Deutschland GmbH, Switzerland
CAAS - Chinese Academy of Agricultural Sciences [CN]
Funding text :
This study was supported by the Key Research and Development Project of Shaanxi Province (Grant number No. 2020NY-017 to QL) and the State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (Grant number No. SKLVEB2019KFKT007 to QL). Acknowledgements
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