[en] [en] BACKGROUND: Giardia duodenalis is an important opportunistic zoonotic intestinal protozoon, which could parasitize yaks. However, a few studies have been conducted on the seasonal infection of G. duodenalis in yaks in China.
METHODS: Overall, 1,027 fecal samples were collected from yaks of two age groups in seven cities of Qinghai Province, China at four seasons between May 2016 and Sep 2017. The prevalence and assemblages were analyzed by nested PCR and multilocus sequence typing (MLST).
RESULTS: The overall prevalence of G. duodenalis was 2.04% (21/1027) based on triose phosphate isomease (tpi) locus. No significant differences in prevalence of the organism in yaks were found among different sampling areas. Additionally, same result was also presented in different seasons. However, there was statistically significant difference between young yaks within 6 months (8.33%, 4/48) and adult yaks over 6 months (1.73%, 17/979). The assemblage A recognized as a zoonotic assemblage (n=3) was found in yaks (>6 months) from Xining, while assemblage E (n=18) was detected from yaks in six cities. There were 5, 2 and 3 G. duodenalis subtypes detected positive at the tpi, the β-giardin (bg), and the glutamate dehydrogenase (gdh) loci, with 2, 2 and 3 novel subtypes, respectively. Three samples were successfully sequenced at all three loci, forming 1 assemblages A multilocus genotype (MLG) and 2 assemblages E MLGs, not reported.
CONCLUSION: This study indicated a zoonotic potential of G. duodenalis in yaks from Qinghai Province and provides basic information about the epidemiology of G. duodenalis.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Song, Jun-Ke; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, China ; State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai Province 810016, China
Wang, Dan; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, China
Ren, Mei ; Université de Liège - ULiège > TERRA Research Centre ; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, China
Yang, Fan; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, China
Wang, Pin-Xue; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, China
Zou, Min; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, China
Zhao, Guang-Hui; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, China
Lin, Qing; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, China ; State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai Province 810016, China
Language :
English
Title :
Seasonal Prevalence and Novel Multilocus Genotypes of Giardia duodenalis in Yaks (Bos grunniens) in Qinghai Province, Western China.
Publication date :
2021
Journal title :
Iranian Journal of Parasitology
ISSN :
1735-7020
eISSN :
2008-238X
Publisher :
Tehran University of Medical Sciences, Iran
Volume :
16
Issue :
4
Pages :
548 - 554
Peer reviewed :
Peer Reviewed verified by ORBi
Funding text :
This study was supported by the Project of Qinghai Science & Technology Department (2016-ZJ-754 and 2016-ZJ-Y01) and the Open Project of State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University (2017-ZZ-08). We thank the owners of the yaks for their collaboration.
Lee MF, Cadogan P, Eytle S, et al. Molecular epidemiology and multilocus sequence analysis of potentially zoonotic Giardia spp. from humans and dogs in Jamaica. Parasitol Res. 2017; 116(1): 409-14.
Di Cristanziano V, Santoro M, Parisi F, et al. Genetic characterization of Giardia duodenalis by sequence analysis in humans and animals in Pemba Island, Tanzania. Parasitol Int. 2014; 63(2): 438-41.
Squire SA, Yang R, Robertson I et al. Molecular characterization of Cryptosporidium and Giardia in farmers and their ruminant livestock from the Coastal Savannah zone of Ghana. Infect Genet Evol. 2017; 55: 236-43.
Zheng G, Alsarakibi M, Liu Y, et al. Genotyp-ing of Giardia duodenalis isolates from dogs in Guangdong, China based on multi-locus se-quence. Korean J Parasitol. 2014; 52(3): 299-304.
Faubert G. Immune response to Giardia duode-nalis. Clin Microbiol Rev. 2000; 13(1): 35-54.
Xiao S, Li G, Wang B, et al. Molecular identification of the first isolate of calf-derived Giardia from mainland of China. Chinese Journal of Zoonoses. 2006; 22(9): 861-3.
Plutzer J, Ongerth J, Karanis P. Giardia taxon-omy, phylogeny and epidemiology: Facts and open questions. Int J Hyg Environ Health. 2010; 213(3): 321-33.
Ryan U, Cacciò SM. Zoonotic potential of Gi-ardia. Int J Parasitol. 2013; 43(12-13): 943-56.
Aloisio F, Filippini G, Antenucci P, et al. Severe weight loss in lambs infected with Giardia duodenalis assemblage B. Vet Parasitol. 2006; 142(1-2):154-8.
Qi M, Cai J, Wang R, et al. Molecular characterization of Cryptosporidium spp. and Giardia du-odenalis from yaks in the central western region of China. BMC Microbiol. 2015; 15: 108.
Jin Y, Fei J, Cai J, et al. Multilocus genotyping of Giardia duodenalis in Tibetan sheep and yaks in Qinghai, China. Vet Parasitol. 2017; 247: 70-6.
Thompson RC. The zoonotic significance and molecular epidemiology of Giardia and giardia-sis. Vet Parasitol. 2004; 126(1-2):15-35.
Feng Y, Xiao L. Zoonotic potential and molecular epidemiology of Giardia species and gi-ardiasis. Clin Microbiol Rev. 2011; 24(1): 110-40.
Asher AJ, Hose G, Power ML. Giardiasis in NSW: Identification of Giardia duodenalis assemblages contributing to human and cattle cases, and an epidemiological assessment of sporadic human giardiasis. Infect Genet Evol. 2016; 44: 157-61.
Song G, Qin S, Zhao G et al. Molecular characterization of Giardia duodenalis from white yaks in China. Acta Parasitol. 2016, 61(2): 397-400.
Wang G, Wang G, Li X et al. Detection of Giardia duodenalis assemblage E infections at the Tibetan Plateau Area: Yaks are suitable hosts. Acta Trop. 2017; 169: 157-62.
Feng Y, Gong X, Zhu K, et al. Prevalence and genotypic identification of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in pre-weaned dairy calves in Guangdong, China. Parasites & Vectors. 2019; 12(1): 41.
Jian Y, Zhang X, Li X, et al. Prevalence and molecular characterization of Giardia duodenalis in cattle and sheep from the Qinghai-Tibetan Plateau Area (QTPA), northwestern China. Vet Parasitol. 2018; 250: 40-4.
Wang G, Wang G, Li X, et al. Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in 1-2-month-old highland yaks in Qinghai Province, China. Par-asitol Res. 2018; 117(6): 1793-800.
Li K, Shahzad M, Zhang H, et al. Socio-economic burden of parasitic infections in yaks from 1984 to 2017 on Qinghai Tibetan Plateau of China-A review. Acta Trop. 2018; 183: 103-9.
Wang H, Zhao G, Chen G, et al. Multilocus genotyping of Giardia duodenalis in dairy cattle in Henan, China. PLoS One. 2014; 9(6): e100453.
Minetti C, Taweenan W, Hogg R et al. Occurrence and diversity of Giardia duodenalis assemblages in livestock in the UK. Transbound Emerg Dis. 2014; 61(6): e60-7.
Hu S, Liu Z, Yan F, et al. Zoonotic and host-adapted genotypes of Cryptosporidium spp., Giardia duodenalis and Enterocytozoon bieneusi in dairy cattle in Hebei and Tianjin, China. Vet Parasitol. 2017; 248: 68-73.
Zhang X, Tan Q, Zhao G, et al. Prevalence, Risk Factors and Multilocus Genotyping of Giardia intestinalis in Dairy Cattle, Northwest Chi-na. J Eukaryot Microbiol. 2016; 63(4): 498-504.
Wang X, Wang R, Ren G et al. Multilocus genotyping of Giardia duodenalis and Enterocyto-zoon bieneusi in dairy and native beef (Qinchuan) calves in Shaanxi province, northwestern China. Parasitol Res. 2016; 115(3): 1355-61.
Fan Y, Wang T, Koehler AV, et al. Molecular investigation of Cryptosporidium and Giardia in pre-and post-weaned calves in Hubei Province, China. Parasites & Vectors volume. 2017; 10(1): 519.
Li J, Wang H, Wang R, et al. Giardia duodenalis Infections in Humans and Other Animals in China. Front Microbiol. 2017; 8: 2004.
Cacciò SM, Ryan, U. Molecular epidemiology of giardiasis. Mol Biochem Parasitol. 2008; 160(2): 75-80.
Sulaiman IM, Fayer R, Bern C, et al. Tri-osephosphate isomerase gene characterization and potential zoonotic transmission of Giardia duodenalis. Emerg Infect Dis. 2003; 9(11): 1444-52.
Gillhuber J, Pallant L, Ash A, et al. Molecular identification of zoonotic and livestock-specific Giardia-species in faecal samples of calves in Southern Germany. Parasit Vectors. 2013; 6(1): 346.
Lalle M, Jimenez-Cardosa E, Cacciò S M, et al. Genotyping of Giardia duodenalis from humans and dogs from Mexico using a beta-giardin nested polymerase chain reaction assay. J Para-sitol. 2005; 91(1): 203-5.
Cacciò S M, Beck R, Lalle M, et al. Multilocus genotyping of Giardia duodenalis reveals striking differences between assemblages A and B. Int J Parasitol. 2008; 38(13): 1523-31.
Wang X, Cai M, Jiang W, et al. High genetic diversity of Giardia duodenalis assemblage E in pre-weaned dairy calves in Shanghai, China, revealed by multilocus genotyping. Parasitol Res. 2017; 116(8): 2101-10.
Uehlinger FD, Greenwood SJ, O'Handley R, et al. Prevalence and genotypes of Giardia duode-nalis in dairy and beef cattle in farms around Charlottetown, Prince Edward Island, Canada. Can Vet J. 2011; 52(9): 967-72.
Kakandelwa C, Siwila J, Nalubamba KS, et al. Prevalence of Giardia in dairy cattle in Lusaka and Chilanga districts, Zambia. Vet Parasitol. 2016; 215: 114-6.
Huang J, Yue D, Qi M, et al. Prevalence and molecular characterization of Cryptosporidium spp. and Giardia duodenalis in dairy cattle in Ningxia, northwestern China. BMC Vet Res. 2014; 10: 292.
Liu G, Su Y, Zhou M, et al. Prevalence and molecular characterization of Giardia duodenalis isolates from dairy cattle in northeast China. Exp Parasitol. 2015; 154: 20-4.
Xiao L, Fayer R. Molecular characterization of species and genotypes of Cryptosporidium and Giardia and assessment of zoonotic transmis-sion. Int J Parasitol. 2008; 38(11): 1239-55.
Volotão AC, Costa-Macedo LM, Haddad FS, et al. Genotyping of Giardia duodenalis from human and animal samples from Brazil using beta-giardin gene: a phylogenetic analysis. Acta Trop. 2007; 102(1): 10-9.
Traversa D, Otranto D, Milillo P, et al. Giardia duodenalis sub-Assemblage of animal and human origin in horses. Infect Genet Evol. 2012; 12(8): 1642-6.
Gomez-Puerta LA, Lopez-Urbina MT, Alar-con V, et al. Occurrence of Giardia duodenalis assemblages in alpacas in the Andean region. Parasitol Int. 2014; 63(1): 31-4.
