[en] Blastocystis is one of the most common intestinal protists in humans and a great number of animals, including sheep and goats. High prevalence and multiple subtypes of Blastocystis have been reported in sheep in several regions of China and elsewhere. However, there is a dearth of knowledge about Blastocystis in Tibetan sheep. A total of 761 fecal samples were collected from Tibetan sheep in seven counties of Qinghai Province, northwestern China, and were examined for the prevalence and subtypes of Blastocystis using molecular technology based on the partial small subunit ribosomal RNA gene of Blastocystis. The overall prevalence of Blastocystis in the investigated Tibetan sheep was 7.5% (57/761) using PCR and DNA Sanger sequencing, and differences in prevalence were observed among the ruminants from the seven counties (P < 0.01), and across four seasons (P < 0.01). Sequence analysis revealed five subtypes (ST14 (57.9%), ST10 (26.3%), ST12 (5.3%), ST21 (5.3%), and ST30 (5.3%)) of Blastocystis sp. in these Tibetan sheep, with ST14 as the predominant subtype. To our knowledge, this is the first report of Blastocystis colonization in Tibetan sheep.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Yang, Fan; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, People's Republic of China, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, People's Republic of China
Gou, Jing-Min; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, People's Republic of China, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, People's Republic of China
Yang, Bing-Ke; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, People's Republic of China
Du, Jia-Yue; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, People's Republic of China
Yao, Hui-Zhong; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, People's Republic of China
Ren, Mei ; Université de Liège - ULiège > TERRA Research Centre ; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, People's Republic of China, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, People's Republic of China
Lin, Qing ; College of Veterinary Medicine, Northwest A&F University, Yangling, Shaanxi Province 712100, People's Republic of China, State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu Province 730046, People's Republic of China, State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, Qinghai Province 810016, People's Republic of China. Electronic address: yllinqing@126.com
Language :
English
Title :
Prevalence and Subtype Distribution of Blastocystis in Tibetan Sheep in Qinghai Province, Northwestern China.
CAAS - Chinese Academy of Agricultural Sciences [CN]
Funding text :
This work was supported by the Key Research and Development Project of Shaanxi Province (grant number 2020NY-017) and the State Key Laboratory of Veterinary Etiological Biology, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences (grant number SKLVEB2019KFKT007).
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