IBR; acute test; copper nanoparticles; fish; neurotransmitter; oxidative stress; Copper; Acetylcholinesterase; Catalase; Biomarkers; Water Pollutants, Chemical; Animals; Copper/toxicity; Acetylcholinesterase/metabolism; Oxidative Stress; Catalase/metabolism; Biomarkers/metabolism; Gills/metabolism; Catfishes; Water Pollutants, Chemical/toxicity; Cellulars; Cu nano-particles; Gill tissues; Integrated biomarker response; Kidney tissues; Liver tissue; Gills; Toxicology; Management, Monitoring, Policy and Law; Health, Toxicology and Mutagenesis; General Medicine
Abstract :
[en] The current study focused on assessing the toxicological effects of copper (Cu) and copper nanoparticles (Cu-NPs) in acute condition on Pangasianodon hypophthalmus. The median lethal concentration (LC50 ) for Cu and Cu-NPs were determined as 8.04 and 3.85 mg L-1 , respectively. For the subsequent definitive test, varying concentrations were selected: 7.0, 7.5, 8.0, 8.5, and 9.0 mg L-1 for Cu, and 3.0, 3.3, 3.6, 3.9, and 4.2 mg L-1 for Cu-NPs. To encompass these concentration levels and assess their toxic effects, biomarkers associated with toxicological studies like oxidative stress, neurotransmission, and cellular metabolism were measured in the liver, kidney, and gill tissues. Notably, during the acute test, the activities of catalase, superoxide dismutase, glutathione-s-transferase, glutathione peroxidase, and lipid peroxide in the liver, gill, and kidney tissues were significantly increased due to exposure to Cu and Cu-NPs. Similarly, acetylcholinesterase activity in the brain was notably inhibited in the presence of Cu and Cu-NPs when compared to the control group. Cellular metabolic stress was greatly influenced by the exposure to Cu and Cu-NPs, evident from the considerable elevation of cortisol, HSP 70, and blood glucose levels in the treated groups. Furthermore, integrated biomarker response, genotoxicity, DNA damage in gill tissue, karyotyping in kidney tissue, and histopathology in gill and liver were investigated, revealing tissue damage attributed to exposure to Cu and Cu-NPs. In conclusion, this study determined that elevated concentrations of essential trace elements, namely Cu and Cu-NPs, induce toxicity and disrupt cellular metabolic activities in fish.
Disciplines :
Environmental sciences & ecology
Author, co-author :
Kumar, Neeraj ; ICAR-National Institute of Abiotic Stress Management, Pune, India
Gismondi, Eric ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Ecologie animale et écotoxicologie
Reddy, Kotha Sammi; ICAR-National Institute of Abiotic Stress Management, Pune, India
Language :
English
Title :
Copper and nanocopper toxicity using integrated biomarker response in Pangasianodon hypophthalmus.
SERB - Science and Engineering Research Board [IN]
Funding text :
The present research was supported by Science and Engineering Research Board, New Delhi, India as an external project (OXX5467). Authors also thankful to the Director, ICAR‐National Institute of Abiotic Stress Management, Baramati, Pune for providing all the facilities for this study.
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