Reference : Unusual traits of the pyoverdin-mediated iron acquisition system in Pseudomonas putid...
Scientific journals : Article
Life sciences : Microbiology
Unusual traits of the pyoverdin-mediated iron acquisition system in Pseudomonas putida strain BTP1.
Ongena, MARC mailto [Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech >]
Jacques, Philippe mailto [Université de Liège - ULiège > Chimie et bio-industries > Bio-industries >]
Delfosse, Philippe [> > > >]
Thonart, Philippe mailto [Université de Liège - ULiège > Gembloux Agro-Bio Tech > Gembloux Agro-Bio Tech - Biochimie et microbiologie industrielles >]
Kluwer Academic Publishers
Yes (verified by ORBi)
The Netherlands
[en] Amino Acid Sequence ; Bacterial Outer Membrane Proteins/chemistry/metabolism ; Biological Transport, Active ; Genes, Bacterial ; Iron/metabolism ; Kinetics ; Molecular Structure ; Oligopeptides ; Pigments, Biological/chemistry/metabolism ; Pseudomonas putida/genetics/growth & development/metabolism ; Receptors, Cell Surface/genetics/metabolism ; Siderophores/chemistry/metabolism ; Species Specificity
[en] Fluorescent Pseudomonas species are characterized by the production of pyoverdin-type siderophores for Fe3+ acquisition in iron-limited environments. Since it produces a structurally specific pyoverdin, Pseudomonas putida strain BTP1 could represent a valuable tool in an attempt to correlate the structural features of these compounds with some specificity in their two main properties i.e. affinity for iron and recognition rate by other Pseudomonas strains. An uncommonly high affinity for iron of the pyoverdin synthetized by P. putida BTP1 was observed by comparing both the apparent stability constant and the decomplexation kinetic of its ferric complex with those of ferripyoverdins from other strains. On another hand, results from growth stimulation experiments and labeled ferripyoverdin uptake assays highlighted the very low recognition rate of BTP1 isopyoverdins by membrane receptors of foreign strains. By contrast, P. putida BTP1 was able to utilize a broad spectrum of structurally unrelated exogenous pyoverdins by means of multiple receptors that are likely constitutively expressed in its outer membrane. The unusual traits of its pyoverdin-mediated iron acquisition system should contribute to enlarge the ecological competence of Pseudomonas putida BTP1 in terms of colonization and persistence in the rhizosphere.

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