B cells; IL-10; decidua; endometrium; implantation; pregnancy; reproductive immunology; uterine mucosa; B-Lymphocytes/immunology; Female; Humans; Pregnancy; Uterus/immunology; B-Lymphocytes; Uterus; Biochemistry, Genetics and Molecular Biology (all); General Biochemistry, Genetics and Molecular Biology
Abstract :
[en] Well-timed interaction of correctly functioning maternal immune cells is essential to facilitate healthy placenta formation, because the uterine immune environment has to tolerate the semi-allogeneic fetus and allow adequate trophoblast invasion. Here, we assess the uterine immune signature before and during pregnancy. Extensive supervised and unsupervised flow cytometry clustering strategies not only show a general increase in immune memory throughout pregnancy but also reveal the continuous presence of B cells. Contrary to the belief that B cells are merely a consequence of uterine pathology, decidual B cells produce IL-10 and are found to be localized in clusters, together with Foxp3pos T cells. Our findings therefore suggest a role for B cells in healthy pregnancy.
Disciplines :
Immunology & infectious disease
Author, co-author :
Benner, Marilen; Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Department of Laboratory Medicine, 6500 HB Nijmegen, the Netherlands. Electronic address: marilen.benner@radboudumc.nl
Feyaerts, Dorien ; Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Department of Laboratory Medicine, 6500 HB Nijmegen, the Netherlands
García, Celia Cartagena; Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Department of Laboratory Medicine, 6500 HB Nijmegen, the Netherlands
Inci Kavum, Nurcan ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Pharmacologie
López, Sergi Cedó; Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Department of Laboratory Medicine, 6500 HB Nijmegen, the Netherlands
Fasse, Esther; Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Department of Laboratory Medicine, 6500 HB Nijmegen, the Netherlands
Shadmanfar, Wijs; MildredClinics Arnhem, 6814 AB Arnhem, the Netherlands
van der Heijden, Olivier W H; Department of Obstetrics and Gynaecology, Radboud University Medical Center, 6500 HB Nijmegen, the Netherlands
Gorris, Mark A J ; Radboud Institute for Molecular Life Sciences, Department of Tumor Immunology, 6500 HB Nijmegen, the Netherlands
Joosten, Irma; Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Department of Laboratory Medicine, 6500 HB Nijmegen, the Netherlands
Ferwerda, Gerben; Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Department of Laboratory Medicine, 6500 HB Nijmegen, the Netherlands
van der Molen, Renate G; Radboud Institute for Molecular Life Sciences, Radboud University Medical Center, Department of Laboratory Medicine, 6500 HB Nijmegen, the Netherlands. Electronic address: renate.vandermolen@radboudumc.nl
Language :
English
Title :
Clusters of Tolerogenic B Cells Feature in the Dynamic Immunological Landscape of the Pregnant Uterus.
RIMLS - Radboud Institute for Molecular Life Sciences [NL]
Funding text :
We thank all women who participated in this study and the staff of MildredHuis. We appreciate all help of Dr. Anita van der Zwan for helpful discussions to optimize placenta dissociation for phenotyping, Daan Zegers during processing, and Niklas Bruse and Rindert Hartman for assistance in data formatting. M.B. is supported by a junior researcher grant of the Radboud Institute for Molecular Life Sciences .
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