Article (Scientific journals)
The apurinic/apyrimidinic endodeoxyribonuclease of rat-liver chromatin.
César, Régis; Verly, Walter
1983In European Journal of Biochemistry, 129 (3), p. 509 - 517
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European Journal of Biochemistry - January 1983 - CÉSAR - The Apurinic Apyrimidinic Endodeoxyribonuclease of Rat‐Liver.pdf
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Keywords :
Chromatin; Isoenzymes; Endodeoxyribonucleases; Deoxyribonuclease IV (Phage T4-Induced); DNA-(Apurinic or Apyrimidinic Site) Lyase; Animals; Binding Sites; Chemical Phenomena; Chemistry; Chromatin/isolation & purification; Endodeoxyribonucleases/isolation & purification; Hydrolysis; Isoenzymes/isolation & purification; Liver/enzymology; Molecular Weight; Rats; Solubility; Biochemistry
Abstract :
[en] The extract from rat liver chromatin contains two apurinic/apyrimidinic (AP) endodeoxyribonucleases named 0.2 M and 0.3 M isozymes according to the phosphate concentration necessary to elute them from an hydroxyapatite column. The 0.3 M isozyme is the main and perhaps the only chromatin AP endodeoxyribonuclease in the living cell. This 0.3 M isozyme was purified by successive chromatographies on hydroxyapatite, phosphocellulose, heparin-Sepharose and alkylated-depurinated DNA-cellulose. It has a molecular weight of approximately 39000; its optimum pH is around 8.0; it needs Mg2+ or Mn2+ to be active and the optimum concentration for Mg2+ is between 5 mM and 10 mM. The 0.3 M isozyme has no action on intact DNA strands or on alkylated sites; it cuts the phosphodiester bridge which is the immediate neighbour of the AP site on its 5' side leaving 3'-hydroxyl and 5'-phosphate ends. It has no associated exonuclease activity. To hydrolyze the phosphoester bond near the AP site, the enzyme makes a close contact with three base residues in the large groove of the DNA molecule.
Disciplines :
Human health sciences: Multidisciplinary, general & others
Author, co-author :
César, Régis;  Laboratoire de Biochimie, Faculté des Sciences, Université de Liège, Liège, B-4000, Belgium
Verly, Walter ;  Relations académiques et scientifiques (Sciences)
Language :
English
Title :
The apurinic/apyrimidinic endodeoxyribonuclease of rat-liver chromatin.
Alternative titles :
[fr] L'endodésoxyribonucléase apurinique/apyrimidinique de la chromatine du foie de rat.
Publication date :
01 January 1983
Journal title :
European Journal of Biochemistry
ISSN :
0014-2956
eISSN :
1432-1033
Publisher :
Wiley, England
Volume :
129
Issue :
3
Pages :
509 - 517
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 March 2024

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