[en] Four Pseudomonas strains were evaluated for their intrinsic properties conferring their ability to protect long English cucumber against Pythium aphanidermatum in hydroponic culture. Two of the strains, BTP1 and its siderophorenegative mutant M3, increased plant yield as compared with the non-inoculated control plants. Strain BTP7 was intermediate in its biocontrol activity while strain ATCC 17400 failed to reduce disease development. The role of pyoverdines could not be confirmed since treatment with either BTP1 or its siderophore-negative mutant M3 provided similar suppression of Pythium disease. In addition, no siderophores were detected in the nutrient solution. BTP1 did not inhibit pathogen growth in vitro on several media, suggesting that antibiosis was not a mechanism of suppression. Quantification of root bacterial populations did not indicate differences among the strains. On the other hand, roots treated with either BTP1 or its sid¹ mutant M3 contained more antifungal phenolics than roots from any other
treatments including controls. These results suggest that antifungal compounds induced by inoculation of cucumber roots with the fluorescent Pseudomonas strains BTP1 and M3 participate actively in the protection of cucumber plants against P. aphanidermatum
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Bibliography
Bakker PAHM, Lamers JG, Bakker AW, Marugg JD, Weisheek PJ, Schippers B, 1986. The role of siderophores in potato tuber yield increase by Pseudomonas pntida in a short rotation of potato. Netherland Journal of Plant Pathology 92, 249-56.
Bakker PAHM, Raaijmakers M, Schippers B, 1993. Role of iron in the suppression of bacterial plant pathogens by fluorescent pseudomonads. In: Barton LL and Hemming BC, eds. Iron Chelation in Plants and Soil Microorganisms. San Diego, USA: Academic Press, 269-81.
Becker JO, Cook RJ, 1988. Role of siderophores in suppression of Pythium species and production of increased-growth response of wheat by fluorescent pseudomonads. Phytopathology 78, 778-82.
Benhamou N, Bélanger RR, 1998. Induction of systemic resistance to Pythium damping-off in cucumber plants by benzothiadiazole: Ultrastructure and cytochemistry of the host response. Plant Journal (in press).
Benhamou N, Bélanger RR, Paulitz TC, 1996. Pre-inoculation of RI T-DNA-transformed pea roots with Pseudomonas fluorescens inhibits colonization by Pythium ultimum trow - An ultrastructural and cytochemical study. Planta 199, 105-17.
Buysens S, Heungens K, Poppe J, Höfte M, 1996. Involvement of pyochelin and pyoverdin in suppression of Pythium-induced damping-off of tomato by Pseudomonas aeruginosa 7NSK2. Applied and Environmental Microbiology 62, 865-71.
Chérif M, Asselin A, Bélanger RR, 1994. Defense responses induced by soluble silicon in cucumber roots infected by Pythium spp. Phytopathology 84, 236-42.
Chérif M, Benhamou N, Menzies JG, Bélanger RR, 1992. Silicon induced resistance in cucumber plants against Pythium ultimum. Physiological and Molecular Plant Pathology 41, 411-25.
Daayf F, Schmitt A, Bélanger RR, 1995. The effects of plant extracts of Reynoutria sachalinensis on powdery mildew development and leaf physiology of long English cucumber. Plant Disease 79, 577-80.
Daayf F, Schmitt A, Bélanger RR, 1997. Evidence of phytoalexins in cucumber leaves infected with powdery mildew following treatment with leaf extracts of Reynoutria sachalinensis. Plant Physiology 113, 719-27.
El Ghaouth A, Arul J, Grenier J, Benhamou N, Asselin A, Bélanger RR, 1994. Effect of chitosan on cucumber plants: Suppression of Pythium aphanidermatum and induction of defense reactions. Phytopathology 84, 313-20.
Elad Y, Chet I, 1987. Possible role of competition for nutrients in biocontrol of Pythium damping-off by bacteria. Phytopathology 77, 190-5.
Fawe A, Abou-Zaid M, Menzies JG, Bélanger RR, 1998. Silicon-mediated accumulation of flavonoid phytoalexins in cucumber. Phytopathology 88, 396-401.
Fridlender M, Inbar J, Chet I, 1993. Biological control of soil-borne plant pathogens by a β-1,3-glucanase-producing Pseudomonas cepacia. Soil Biology and Biochemistry 25, 1211-21.
Gamard P, Sauriol F, Benhamou N, Bélanger RR, Paulitz TC, 1997. Novel butyrolactones with antifungal activity produced by Pseudomonas aureofaciens strain 63-28. Journal of Antibiotics 50, 742-9.
Gill PR, Warren JR, Warren GJ, 1988. An iron-antagonized fungistatic agent that is not required for iron assimilation from a fluorescent rhizosphere pseudomonad. Journal of Bacteriology 170, 163-70.
Glick BR, 1995. The enhancement of plant-growth by freeliving bacteria. Canadian Journal of Microbiology 41, 109-17.
Gutterson N, 1990. Microbial fungicides: recent approaches to elucidating mechanisms. Critical Reviews in Biotechnology 10, 69-91.
Hammerschmidt R, Kúc J, 1995. Induced Resistance to Disease in Plants. Dordrecht, The Nederlands: Kluwers Academic Publishers.
Howell CR, Stipanovic RD, 1979. Control of Rhizoctonia solani on cotton seedlings with Pseudomonas fluorescens and with an antibiotic produced by the bacterium. Phytopathology 69, 480-2.
Howie WJ, Suslow TV, 1991. Role of antibiotic biosynthesis in the inhibition of Pythium ultimum in the cotton spermosphere and rhizosphere by Pseudomonas fluorescens. Molecular Plant-Microbe Interactions 4, 393-9.
Jacques P, Delfosse P, Ongena M, Lepoivre P, Cornélis P, Koedam N, Neirinckx L, Thonart P, 1993a. Les mécanismes biochimiques développés par les Pseudomonas fluorescents dans la lutte biologique contre les maladies des plantes transmises par le sol. Cahiers Agricultures 2, 301-7.
Jacques P, Hbid C, Ongena M, Delfosse P, Cornélis P, Koedam N, Lepoivre P, Razafindralambo H, Paquot M, Thonart P, 1993c. In vitro antagonism of two potential biopesticides Bacillus subtilis and Pseudomonas fluorescens: biochemical aspects. In: Lepoivre P, ed. Proceedings of an EC Workshop: Biological Control of Fruit and Foliar Diseases. Gembloux, Belgium, European Agriculture Commission, 55-60.
Jacques P, Mistry C, Ongena M, Cornélis P, Anjaiah V, Koedam N, Neirinckx L, Lepoivre P, 1993b. Study of the antagonism developed by fluorescent Pseudomonas against Pythium spp. using Tn5 and NTG mutants. Archives Internationales de Physiologie et de Biochimie 101, B21.
Jacques P, Ongena M, Gwose I, Seinsche D, Schröder H, Delfosse P, Thonart P, Taraz K, Budzikiewicz H, 1995. Structure and characterization of isopyoverdin from Pseudomonas putida BTP1 and its relation to the biogenetic pathway leading to pyoverdins. Zeitschrift für Naturforschung 50c, 622-9.
Keel C, Wirthner PH, Oberhansli TH, Voisard C, Burger D, Haas D, Défago G, 1990. Pseudomonads as antagonists of plant pathogens in the rhizosphere: role of the antibiotic 2,4-diacetylphloroglucinol in the suppression of black root rot of tobacco. Symbiosis 9, 327-41.
King EO, Ward MK, Raney DE, 1954. Two simple media for the demonstration of pyocyanin and fluorescein. Journal of Laboratory and Clinical Medicine 44, 301-7.
Kloepper WJ, Leong J, Teintze M, Schroth MN, 1980. Pseudomonas siderophores: a mechanism explaining disease suppressive soils. Nature 286, 885-8.
Kraus J, Loper JE, 1992. Lack of evidence for a role of antifungal metabolite production by Pseudomonas fluorescens Pf-5 in biological control of Pythium damping-off of cucumber. Phytopathology 82, 264-71.
Leeman M, den Ouden FM, Van Pelt JA, Dirkx FPM, Steijl H, Bakker PAHM, Schippers B, 1996. Iron availability affects induction of systemic resistance to Fusarium wilt of radish by Pseudomonas fluorescens. Phytopathology 86, 149-55.
Leeman M, van Pelt JA, den Ouden FM, Heinsbrock M, Bakker PAHM, Schippers B, 1995. Induction of systemic resistance against Fusarium wilt of radish by lipopolysaccharides of Pseudomonas fluorescens. Phytopathology 85, 1021-7.
Liu L, Kloepper JW, Tuzun S, 1995a. Induction of systemic resistance in cucumber against Fusarium wilt by plant growth-promoting rhizobacteria. Phytopathology 85, 695-8.
Liu L, Kloepper JW, Tuzun S, 1995b. Induction of systemic resistance in cucumber against bacterial angular leaf spot by plant growth-promoting rhizobacteria. Phytopathology 85, 843-7.
Loper JE, Buyer JS, 1991. Siderophores in microbial interactions on plant surfaces. Molecular Plant-Microbe Interactions 4, 5-13.
Maurhofer M, Hase C, Meuwly P, Métraux JP, Défago G, 1994. Induction of systemic resistance of tobacco to tobacco necrosis virus by the root-colonizing Pseudomonas fluorescens strain CHA0: Influence of the gacA gene and of pyoverdine production. Phytopathology 84, 139-46.
Meyer JM, Abdallah MA, 1978. The fluorescent pigment of Pseudomonas fluorescens: biosynthesis, purification and physico-chemical properties. Journal of General Microbiology 107, 319-28.
Neilands JB, 1986. Siderophores in relation to plant growth and disease. Annual Review of Plant Physiology 37, 187-208.
O'Sullivan DJ, O'Gara F, 1992. Traits of fluorescent Pseudomonas spp. involved in suppression of plant root pathogens. Microbiological Reviews 56, 662-76.
Ongena M, 1996. Etude de Siderophores de Pseudomonas Fluorescents en Relation avec la Lutte Biologique. Liege, Belgium: University of Liege, Belgium, PhD thesis.
Paulitz TC, Loper JE, 1991. Lack of a role for fluorescent siderophore production in the biological control of Pythium damping-off of cucumber by a strain of Pseudomonas putida. Phytopathology 81, 930-5.
Schippers B, 1992. Prospects for management of natural suppressiveness to control soilborne pathogens. In: Tjamos EC, Papavizas GC, Cook RJ, eds. Biological Control of Plant Diseases, Progress and Challenges for the Future. New York, USA: Plenum Press, 21-34. (NASO ASI Series, Series A: Life Sciences, vol 230).
Schippers B, Bakker AW, Bakker PAHM, 1987. Interactions of deleterious and beneficial rhizosphere microorganisms and the effect of cropping practices. Annual Review of Phytopathology 25, 339-58.
Schwyn B, Neilands JB, 1987. Universal chemical assay for the detection and determination of siderophores. Analytical Biochemistry 160, 47-56.
Thomashow LS, Weller DM, 1988. Role of a phenazine antibiotic from Pseudomonas fluorescens in biological control of Gaeumannomyces graminis var. tritici. Journal of Bacteriology 170, 3499-508.
Thomashow LS, Weller DM, 1995. Current concepts in the use of introduced bacteria for biological control: mechanisms and antifungal metabolites. In: Stacey G, Keen N, eds. Plant-Microbe Interactions, vol.1. New York, USA: Chapman and Hall. 187-235.
Wei G, Kloepper JW, Tuzun S, 1991. Induction of systemic resistance of cucumber to Colletotrichum orbiculare by select strains of plant growth-promoting rhizobacteria. Phytopathology 81, 1508-12.
Weller DM, Howie WJ, Cook RJ, 1988. Relationship between in vitro inhibition of Gaeumannomyces graminis var. tritici and suppression of take-all of wheat by fluorescent pseudomonads. Phytopathology 78, 1094-100.
Zhou T, Paulitz TC, 1994. Induced resistance in the biocontrol of Pythium aphanidermatum by Pseudomonas spp. on cucumber. Journal of Phytopathology 142, 51-63.
Zhou T, Rankin L, Paulitz TC, 1992. Induced resistance in the biological control of Pythium aphanidermatum by Pseudomonas spp. on European cucumber (Abstract). Phytopathology 82, 1080.
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