Identification of fabclavine derivatives, Fcl-7 and Fcl-8, from Xenorhabdus budapestensis as major antifungal natural products against Rhizoctonia solani.
Yuan, Baoming; Li, Beibei; Shen, Hongfeiet al.
2023 • In Journal of Applied Microbiology, 134 (9)
Rhizoctonia solani; Xenorhabdus budapestensis; biofungicide; fabclavine; Antifungal Agents; Biological Products; Humans; Xenorhabdus; Dandruff; Antifungals; Biofungicides; Cell-free; Natural products; R. Solani; Supernatants; Wing area; Xenorhabdu budapestensis; Biotechnology; Applied Microbiology and Biotechnology; General Medicine
Abstract :
[en] [en] AIMS: Black scurf disease, caused by Rhizoctonia solani, is a severe soil-borne and tuber-borne disease, which occurs and spreads in potato growing areas worldwide and poses a serious threat to potato production. New biofungicide is highly desirable for addressing the issue, and natural products (NPs) from Xenorhabdus spp. provide prolific resources for biofungicide development. In this study, we aim to identify antifungal NPs from Xenorhabdus spp. for the management of this disease.
METHODS AND RESULTS: Out of the 22 Xenorhabdus strains investigated, Xenorhabdus budapestensis 8 (XBD8) was determined to be the most promising candidate with the measured IC50 value of its cell-free supernatant against R. solani as low as 0.19 ml l-1. The major antifungal compound in XBD8 started to be synthesized in the middle logarithmic phase and reached a stable level at stationary phase. Core gene deletion coupled with high-resolution mass spectrometry analysis determined the major antifungal NPs as fabclavine derivatives, Fcl-7 and 8, which showed broad-spectrum bioactivity against important pathogenic fungi. Impressively, the identified fabclavine derivatives effectively controlled black scurf disease in both greenhouse and field experiments, significantly improving tuber quality and increasing with marketable tuber yield from 29 300 to 35 494 kg ha-1, comparable with chemical fungicide fludioxonil.
CONCLUSIONS: The fabclavine derivatives Fcl-7 and 8 were determined as the major antifungal NPs in XBD8, which demonstrated a bright prospect for the management of black scurf disease.
Disciplines :
Agriculture & agronomy
Author, co-author :
Yuan, Baoming; State Key Laboratory for Biology of Plant Diseases and Insect Pests/Key Laboratory of Control of Biological Hazard Factors (Plant Origin) for Agri-product Quality and Safety, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China ; College of Plant Protection, Hebei Agricultural University, Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province, Baoding 071000, China
Li, Beibei ; State Key Laboratory for Biology of Plant Diseases and Insect Pests/Key Laboratory of Control of Biological Hazard Factors (Plant Origin) for Agri-product Quality and Safety, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China ; Functional and Evolutionary Entomology, Gembloux Agro-Bio Tech, University of Liège, B-5030 Gembloux, Belgium
Shen, Hongfei; State Key Laboratory for Biology of Plant Diseases and Insect Pests/Key Laboratory of Control of Biological Hazard Factors (Plant Origin) for Agri-product Quality and Safety, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Duan, Jiaqi; State Key Laboratory for Biology of Plant Diseases and Insect Pests/Key Laboratory of Control of Biological Hazard Factors (Plant Origin) for Agri-product Quality and Safety, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Jia, Fenglian; State Key Laboratory for Biology of Plant Diseases and Insect Pests/Key Laboratory of Control of Biological Hazard Factors (Plant Origin) for Agri-product Quality and Safety, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Maimaiti, Yushanjiang; Key Laboratory of Integrated Pest Management on Crops in Northwestern Oasis, Ministry of Agriculture and Rural Affairs, Research Institute of plant protection Xinjiang Academy of Agricultural Sciences, Urumqi, Xinjiang, 83009, China
Li, Yaning; College of Plant Protection, Hebei Agricultural University, Technological Innovation Center for Biological Control of Crop Diseases and Insect Pests of Hebei Province, Baoding 071000, China
Li, Guangyue; State Key Laboratory for Biology of Plant Diseases and Insect Pests/Key Laboratory of Control of Biological Hazard Factors (Plant Origin) for Agri-product Quality and Safety, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Language :
English
Title :
Identification of fabclavine derivatives, Fcl-7 and Fcl-8, from Xenorhabdus budapestensis as major antifungal natural products against Rhizoctonia solani.
We thank the support from the Key Research and Develop- ment Program Project of Xinjiang (2022B02044), the Na- tional Natural Science Foundation of China (Grant No. 31972327), the Agricultural Science and Technology Innova- tion Program of CAAS (C AAS-ZDRW202011) and (CAAS- ZDR W202002). We also acknowledge the support from the Special Project on Regional Collaborative Innovation of Xin- jiang Uygur Autonomous Region (Plan for Assisting Xinjiang with Science and Technology) (2021E02024), the Natural Science Foundation of Xinjiang Uygur Autonomous Region (2022D01F069), and the Project of Fund for Stable Support to Agricultural Sci-Tech Renovation "Research and development of key technologies for prevention and control of agricultural diseases, pests, weeds, and biosafety"(xjnkywdzc-2022004).We thank the support from the Key Research and Development Program Project of Xinjiang (2022B02044), the National Natural Science Foundation of China (Grant No. 31972327), the Agricultural Science and Technology Innovation Program of CAAS (CAAS-ZDRW202011) and (CAAS-ZDRW202002). We also acknowledge the support from the Special Project on Regional Collaborative Innovation of Xinjiang Uygur Autonomous Region (Plan for Assisting Xinjiang with Science and Technology) (2021E02024), the Natural Science Foundation of Xinjiang Uygur Autonomous Region (2022D01F069), and the Project of Fund for Stable Support to Agricultural Sci-Tech Renovation “Research and development of key technologies for prevention and control of agricultural diseases, pests, weeds, and biosafety” (xjnkywdzc-2022004).
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