[en] The present study was conducted to evaluate the protective effect of milk kefir against NSAID-induced gastric ulcers. Male Swiss mice were divided into three groups: control (Vehicle; UHT milk at a dose of 0.3 mL/100 g), proton pump inhibitor (PPI; lansoprazole 30 mg/kg), and 4% milk kefir (Kefir; 0.3 mL/100 g). After 14 days of treatment, gastric ulcer was induced by oral administration of indomethacin (40 mg/kg). Reactive oxygen species (ROS), nitric oxide (NO), DNA content, cellular apoptosis, IL-10 and TNF-α levels, and myeloperoxidase (MPO) enzyme activity were determined. The interaction networks between NADPH oxidase 2 and kefir peptides 1-35 were determined using the Residue Interaction Network Generator (RING) webserver. Pretreatment with kefir for 14 days prevented gastric lesions. In addition, kefir administration reduced ROS production, DNA fragmentation, apoptosis, and TNF-α systemic levels. Simultaneously, kefir increased NO bioavailability in gastric cells and IL-10 systemic levels. A total of 35 kefir peptides showed affinity with NADPH oxidase 2. These findings suggest that the gastroprotective effect of kefir is due to its antioxidant and anti-inflammatory properties. Kefir could be a promising natural therapy for gastric ulcers, opening new perspectives for future research.
Disciplines :
Chemistry
Author, co-author :
Côco, Larissa Zambom; Laboratory of Translational Physiology and Pharmacology, Pharmaceutical Sciences Graduate Program, Vila Velha University (UVV), Vila Velha 29102-920, ES, Brazil
Aires, Rafaela; Laboratory of Translational Physiology and Pharmacology, Pharmaceutical Sciences Graduate Program, Vila Velha University (UVV), Vila Velha 29102-920, ES, Brazil
Carvalho, Glaucimeire Rocha; Laboratory of Translational Physiology and Pharmacology, Pharmaceutical Sciences Graduate Program, Vila Velha University (UVV), Vila Velha 29102-920, ES, Brazil
Belisário, Eduarda de Souza; Laboratory of Translational Physiology and Pharmacology, Pharmaceutical Sciences Graduate Program, Vila Velha University (UVV), Vila Velha 29102-920, ES, Brazil
Yap, Michelle Khai Khun ; School of Science, Monash University Malaysia, Bandar Sunway 47500, Selangor, Malaysia
Gobbi Amorim, Fernanda ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)
Conde-Aranda, Javier ; Molecular and Cellular Gastroenterology, Health Research Institute of Santiago de Compostela (IDIS), 15706 Santiago de Compostela, Spain
Nogueira, Breno Valentim; Department of Morphology, Health Sciences Center, Federal University of Espírito Santo (UFES), Vitoria 29047-105, ES, Brazil
Vasquez, Elisardo Corral ; Laboratory of Translational Physiology and Pharmacology, Pharmaceutical Sciences Graduate Program, Vila Velha University (UVV), Vila Velha 29102-920, ES, Brazil
Pereira, Thiago de Melo Costa; Laboratory of Translational Physiology and Pharmacology, Pharmaceutical Sciences Graduate Program, Vila Velha University (UVV), Vila Velha 29102-920, ES, Brazil
Campagnaro, Bianca Prandi ; Laboratory of Translational Physiology and Pharmacology, Pharmaceutical Sciences Graduate Program, Vila Velha University (UVV), Vila Velha 29102-920, ES, Brazil
Language :
English
Title :
Unravelling the Gastroprotective Potential of Kefir: Exploring Antioxidant Effects in Preventing Gastric Ulcers.
Publication date :
08 December 2023
Journal title :
Cells
eISSN :
2073-4409
Publisher :
Multidisciplinary Digital Publishing Institute (MDPI), Basel, Switzerland
CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico [BR] Xunta de Galicia [ES]
Funding text :
This work was supported by Espírito Santo Research Foundation (FAPES) and by the National Council for the Development of Science and Technology (CNPq). FAPES supported BPC (Edital 21/2018—Universal: 120/2019; Edital 15/2022—PROFIX: 630/2022; Edital 02/2023—Taxa de Pesquisa: 317/2023) and TMCP (Edital 15/2022—PROFIX: 708/2022; Edital 20/2022—Chamada de Apoio a Núcleos Capixabas Emergentes em Pesquisa: 1042/2022). CNPq supported BPC (308220/2021-8), TMCP (309431/2022-0) and ECV (305740/2019-9). Xunta de Galicia: Plan Galego IDT, 2021–2022, M.C.-T., supported ECV (ED431B 2020/26e).
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