[en] Palearctic water frogs (genus Pelophylax) are an outstanding model in ecology and evolution, being widespread, speciose, either threatened or threatening to other species through biological invasions, and capable of siring hybrid offspring that escape the rules of sexual eproduction. Despite half a century of genetic research and hundreds of publications, the diversity, systematics and biogeography of Pelophylax still remain highly confusing, in no small part due to a lack of correspondence between studies. To provide a comprehensive overview, we gathered >13,000 sequences of barcoding genes from >1700 native and introduced localities and built multigene mitochondrial (~17 kb) and nuclear (~10 kb) phylogenies. We mapped all currently recognized taxa and their phylogeographic lineages (>40) to get a grasp on taxonomic issues, cyto-nuclear discordances, the genetic makeup of hybridogenetic hybrids, and the origins of introduced populations. Competing hypotheses for the molecular calibration were evaluated through plausibility tests, implementing a new approach relying on predictions from the anuran speciation continuum. Based on our timetree, we propose a new biogeographic paradigm for the Palearctic since the Paleogene, notably by attributing a prominent role to the dynamics of the Paratethys, a vast paleo-sea
that extended over most of Europe. Furthermore, our results show that distinct marsh frog
lineages from Eastern Europe, the Balkans, the Near East, and Central Asia (P. ridibundus ssp.) are naturally capable of inducing hybridogenesis with pool frogs (P. lessonae). We identified 14 alien lineages (mostly of P. ridibundus) over ~20 areas of invasions, especially in Western Europe, with genetic signatures disproportionally pointing to the Balkans and Anatolia as the regions of origins, in line with exporting records of the frog leg industry and the stocks of pet sellers. Pelophylax thus emerges as one of the most invasive amphibians worldwide, and deserves much higher conservation
Research Center/Unit :
FOCUS - Freshwater and OCeanic science Unit of reSearch - ULiège [BE]
Dufresnes, Christophe; Laboratory of Amphibian Systematics and Evolutionary Research (LASER), College of Biology and the Environment, Nanjing Forestry University, Nanjing, People's Republic of China ; Institut de Systématique, Muséum national d'Histoire naturelle, Evolution ; CNRS, Sorbonne Université, EPHE, Université des Antilles, Paris, France
Monod-Broca, Benjamin; UMR 5023 LEHNA, Université Claude Bernard Lyon 1, CNRS, ENTPE, Villeurbanne, France
Bellati, Adriana; Department of Ecological and Biological Sciences, University of Tuscia, Viterbo, Italy
Canestrelli, Daniele; Department of Ecological and Biological Sciences, University of Tuscia, Viterbo, Italy
Ambu, Johanna; Laboratory of Amphibian Systematics and Evolutionary Research (LASER), College of Biology and the Environment, Nanjing Forestry University, Nanjing, People's Republic of China
Wielstra, Ben; Institute of Biology Leiden, Leiden University, Leiden, The Netherlands ; Naturalis Biodiversity Center, Leiden, The Netherlands
Dubey, Sylvain; Department of Ecology and Evolution, University of Lausanne, Lausanne, Switzerland
Crochet, Pierre-André; CEFE, CNRS, Univ Montpellier, EPHE, Montpellier, France
Denoël, Mathieu ✱; Université de Liège - ULiège > Freshwater and OCeanic science Unit of reSearch (FOCUS) ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Laboratoire d'Écologie et de Conservation des Amphibiens (LECA)
Jablonski, Daniel ✱; Department of Zoology, Comenius University in Bratislava, Bratislava, Slovakia
✱ These authors have contributed equally to this work.
Language :
English
Title :
Piecing the barcoding puzzle of Palearctic water frogs (Pelophylax) sheds light on amphibian biogeography and global invasions
Publication date :
2024
Journal title :
Global Change Biology
ISSN :
1354-1013
eISSN :
1365-2486
Publisher :
Blackwell, Oxford, United Kingdom
Volume :
30
Issue :
3
Pages :
e17180
Peer reviewed :
Peer Reviewed verified by ORBi
Development Goals :
14. Life below water
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
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