Electron paramagnetic spectroscopy; Ion-substituted hydroxyapatite; Magnesium; Saline; Simulated body fluid; Chemical Engineering (all); Materials Science (all); Condensed Matter Physics; Inorganic Chemistry; General Materials Science; General Chemical Engineering
Abstract :
[en] To assess the application potential of novel biomaterials, their behaviour in model media and upon sterilization should be investigated, as well as the stability related to their storage condi-tions. Such data are lacking for Mg-substituted HAP (Mg-HAP). Therefore, the changes in the local structure of non-substituted and Mg-HAP after irradiation and immersion in corrected simulated fluid and saline solution for 28 days were followed by electron paramagnetic resonance (EPR) spectroscopy for the first time. To better understand the stability of radical species induced by steriliza-tion, EPR spectra of samples kept for 2 h at temperatures up to 373 K were recorded to provide an insight into the stability of the sample storage conditions by the accelerated aging method. Samples were characterized by PXRD, FTIR, SEM, EDS, AAS and TGA. Results confirmed that irradiation does not induce changes in the composition or the structure of any of the investigated materials. Fading or the complete disappearance of radical signals in the EPR spectra after immersion in both media was accompanied by the disappearance of other phases formed as a minor byproduct in the synthesis of substituted HAP, as confirmed by PXRD and FTIR analysis. Obtained results confirm the great potential of Mg-HAPs for biomedical applications, although closer attention should be given to the processes related to sample storage stability at different temperatures.
Disciplines :
Chemistry
Author, co-author :
Vidotto, Monica; Laboratory for Electron Spin Spectroscopy, Division of Physical Chemistry, Ruđer Bošković Institute, Zagreb, Croatia
Grego, Timor; Department of Occupational Safety and Health, Fire and Radiation Protection Ruđer Bošković Institute, Zagreb, Croatia ; Department of Urology, University Hospital Centre Zagreb, Zagreb, Croatia
Petrović, Božana ; Laboratory for Atomic Physics, Vinča Institute of Nuclear Sciences—National Institute of the Republic of Serbia, Belgrade University, Vinča, Belgrade, Serbia
Somers, Nicolas ; Université de Liège - ULiège ; FR CERAMATHS Département Matériaux et Procédés, Université Polytechnique Hauts-de-France (UPHF), Maubeuge, France
Antonić Jelić, Tatjana ; Laboratory for Synthesis of New Materials, Division of Materials Chemistry, Ruđer Bošković Institute, Zagreb, Croatia
Kralj, Damir; Laboratory for Precipitation Processes, Division of Materials Chemistry, Ruđer Bošković Institute, Zagreb, Croatia
Matijaković Mlinarić, Nives ; Laboratory for Precipitation Processes, Division of Materials Chemistry, Ruđer Bošković Institute, Zagreb, Croatia
Leriche, Anne ; FR CERAMATHS Département Matériaux et Procédés, Université Polytechnique Hauts-de-France (UPHF), Maubeuge, France
Dutour Sikirić, Maja ; Laboratory for Biocolloids and Surface Chemistry, Division of Physical Chemistry, Ruđer Bošković Institute, Zagreb, Croatia
Erceg, Ina; Laboratory for Biocolloids and Surface Chemistry, Division of Physical Chemistry, Ruđer Bošković Institute, Zagreb, Croatia
Maltar-Strmečki, Nadica ; Laboratory for Electron Spin Spectroscopy, Division of Physical Chemistry, Ruđer Bošković Institute, Zagreb, Croatia
Language :
English
Title :
A Comparative EPR Study of Non-Substituted and Mg-Substituted Hydroxyapatite Behaviour in Model Media and during Accelerated Ageing
HRZZ - Croatian Science Foundation Cogito Foundation Ministry of Science, Education and Sports
Funding text :
Acknowledgments: D.K. and N.M.M. gratefully acknowledges financial support from the Croatian Science Foundation Grant HRZZ-IP-2013-11-5055.Funding: This study was supported by the Croatian Science Foundation, Grant HRZZ-IP-2018-01-1493, Ministry of Science and Education, Croatian-Serbian bilateral project “Ion substituted hydroxyapatites for bone tissue engineering” and COGITO project “Systematic investigation of adsorption of proteins on calcium phosphate based bioceramics for bone tissue engineering”.
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