[en] Rice blast and bacterial blight, caused by the fungus Magnaporthe oryzae and the bacterium Xanthomonas oryzae pv. oryzae (Xoo), respectively, are devastating diseases affecting rice. Here, we report that a rice valine-glutamine (VQ) motif-containing protein, OsVQ25, balances broad-spectrum disease resistance and plant growth by interacting with a U-Box E3 ligase, OsPUB73, and a transcription factor, OsWRKY53. We show that OsPUB73 positively regulates rice resistance against M. oryzae and Xoo by interacting with and promoting OsVQ25 degradation via the 26S proteasome pathway. Knockout mutants of OsVQ25 exhibit enhanced resistance to both pathogens without a growth penalty. Furthermore, OsVQ25 interacts with and suppresses the transcriptional activity of OsWRKY53, a positive regulator of plant immunity. OsWRKY53 downstream defense-related genes and brassinosteroid signaling genes are upregulated in osvq25 mutants. Our findings reveal a ubiquitin E3 ligase-VQ protein-transcription factor module that fine-tunes plant immunity and growth at the transcriptional and posttranslational levels.
Disciplines :
Agriculture & agronomy
Author, co-author :
Hao, Zeyun ✱; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Tian, Jinfu ✱; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Fang, Hong ✱; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China, College of Agronomy, Hunan Agricultural University, Changsha 410128, China
Fang, Liang; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Xu, Xiao; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
He, Feng; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Li, Shaoya; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China
Xie, Wenya; Key Laboratory of Plant Functional Genomics of the Ministry of Education/ Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Agricultural College of Yangzhou University, Yangzhou 225009, China
Du, Qiang; College of Agronomy, Hunan Agricultural University, Changsha 410128, China
You, Xiaoman; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Wang, Debao; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Chen, Qiuhong; College of Agronomy, Hunan Agricultural University, Changsha 410128, China
Wang, Ruyi; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China
Zuo, Shimin; Key Laboratory of Plant Functional Genomics of the Ministry of Education/ Jiangsu Key Laboratory of Crop Genomics and Molecular Breeding, Agricultural College of Yangzhou University, Yangzhou 225009, China
Yuan, Meng; National Key Laboratory of Crop Genetic Improvement, National Center of Plant Gene Research, Huazhong Agricultural University, Wuhan 430070, China
Wang, Guo-Liang; Department of Plant Pathology, The Ohio State University, Columbus, OH 43210, USA
Xia, Lanqin; Institute of Crop Sciences, Chinese Academy of Agricultural Sciences, Beijing 100081, China, Hainan Yazhou Bay Seed Laboratory/National Nanfan Research Institute (Sanya), Chinese Academy of Agricultural Sciences, Sanya 572024, China. Electronic address: xialanqin@caas.cn
Ning, Yuese ; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China. Electronic address: ningyuese@caas.cn
NSCF - National Natural Science Foundation of China
Funding text :
This work was supported by grants from the National Natural Science Foundation of China ( 32161143009 , 31822041 , and 31972225 ) to Y.N., the Major Research Plan of the National Natural Science Foundation of China ( 3218810004 ) to L.X., and the National Natural Science Foundation of China ( U20A2021 ) to R.W.
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