[en] The song control nucleus HVC of songbirds has emerged as a widespread model system to study adult neurogenesis and the factors that modulate the incorporation of new neurons, including seasonal state, sex differences or sex steroid hormone concentrations. However, the specific function of these new neurons born in adulthood remains poorly understood. We implemented a new procedure based on focal X-ray irradiation to deplete neural progenitors in the ventricular zone adjacent to HVC and study the functional consequences. A 23 Gy dose depleted by more than 50 percent the incorporation of BrdU in neural progenitors, a depletion that was confirmed by a significant decrease in doublecortin positive neurons. This depletion of neurogenesis significantly increased the variability of testosterone-induced songs in females and decreased their bandwidth. Expression of the immediate early gene ZENK in secondary auditory areas of the telencephalon that respond to song was also inhibited. These data provide evidence that new neurons in HVC play a role in both song production and perception and that X-ray focal irradiation represents an excellent tool to advance our understanding of adult neurogenesis.
Research Center/Unit :
GIGA Neurosciences-Neuroendocrinology - ULiège
Disciplines :
Neurosciences & behavior
Author, co-author :
Chiver, Ioana ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques
Dos Santos, Ednei B; GIGA Neurosciences, University of Liege, 15 Avenue Hippocrate, 4000, Liège, Belgium
NINDS - National Institute of Neurological Disorders and Stroke [US-MD] F.R.S.-FNRS - Fonds de la Recherche Scientifique [BE]
Funding text :
This work was supported by a Grant from the National Institute of Neurological Disorders and Stroke (Grant RO1NS104008) to G.F.B., J.B., and C.A.C. We thank Pr. Robert Dooling and Ed. Smith, department of Psychology, University of Maryland in College Park for providing the MATLAB script used to analyze canary song. C.A.C. is F.R.S.-FNRS Research Director.
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