Genetic diversity of Echinococcus multilocularis specimens isolated from Belgian patients with alveolar echinococcosis using EmsB microsatellites analysis.
[en] The genetic diversity of Echinococcus multilocularis (E. multilocularis) specimens isolated from patients with alveolar echinococcosis (AE), is a major field of investigation to correlate with sources of infection, clinical manifestations and prognosis of the disease. Molecular markers able to distinguish samples are commonly used worldwide, including the EmsB microsatellite. Here, we report the use of the EmsB microsatellite polymorphism data mining for the retrospective typing of Belgian specimens of E. multilocularis infecting humans. A total of 18 samples from 16 AE patients treated between 2006 and 2021 were analyzed through the EmsB polymorphism. Classification of specimens was performed through a dendrogram construction in order to compare the similarity among Belgian samples, some human referenced specimens on the EWET database (EmsB Website for the Echinococcus Typing) and previously published EmsB profiles from red foxes circulating in/near Belgium. According to a comparison with human European specimens previously genotyped in profiles, the 18 Belgian ones were classified into three EmsB profiles. Four specimens could not be assigned to an already known profile but some are near to EWET referenced samples. This study also highlights that some specimens share the same EmsB profile with profiles characterized in red foxes from north Belgium, the Netherlands, Luxembourg and French department near to the Belgian border. Furthermore, Belgian specimens present a genetic diversity and include one profile that don't share similarities with the ones referenced in the EWET database. However, at this geographical scale, there is no clear correlation between EmsB profiles and geographical location. Further studies including additional clinical samples and isolates from foxes and rodents of south Belgium are necessary to better understand the spatial and temporal circumstances of human infections but also a potential correlation between EmsB profiles and parasite virulence.
Disciplines :
Radiology, nuclear medicine & imaging
Author, co-author :
Sacheli, Rosalie ; Centre Hospitalier Universitaire de Liège - CHU > > Service de microbiologie clinique
Knapp, Jenny; University of Franche-Comté Laboratory Chrono-environment UMR CNRS 6249, Besançon, France, University Hospital of Besançon, Department of Parasitology-Mycology, National Reference Center Echinococcosis, Besançon, France
PHOLIEN, Caroline ; Centre Hospitalier Universitaire de Liège - CHU > > Service de microbiologie clinique
EGREK, Sabrina ; Centre Hospitalier Universitaire de Liège - CHU > > Service de microbiologie clinique
Léonard, Philippe ; Centre Hospitalier Universitaire de Liège - CHU > > Service des maladies infectieuses - médecine interne
Giot, Jean-Baptiste ; Centre Hospitalier Universitaire de Liège - CHU > > Service des maladies infectieuses - médecine interne
Delaere, Bénédicte; EchinoLiege, University Hospital of Liege (CHU-ULg), Liege, Belgium, Catholic University of Louvain, CHU-UCL Namur, Department of infectiology, Yvoir, Belgium
BLETARD, Noëlla ; Centre Hospitalier Universitaire de Liège - CHU > > Service d'anatomie et cytologie pathologiques
Gofflot, Stéphanie ; Centre Hospitalier Universitaire de Liège - CHU > > Biothèque Hospitalo-Universitaire de Liège (BHUL)
Nollevaux, Marie-Cécile; Catholic University of Louvain, CHU-UCL Namur, Department of Pathology, Yvoir, Belgium
Meunier, Paul ; Centre Hospitalier Universitaire de Liège - CHU > > Service médical de radiodiagnostic
Lovinfosse, Pierre ; Centre Hospitalier Universitaire de Liège - CHU > > Service médical de médecine nucléaire et imagerie onco
Pirotte, Benoit; CHR Citadelle Hospital, Department of Infectious Diseases, Liege, Belgium
Truyens, Carine; Free University of Brussels, Department of parasitology, Brussels, Belgium
Millon, Laurence; University of Franche-Comté Laboratory Chrono-environment UMR CNRS 6249, Besançon, France, University Hospital of Besançon, Department of Parasitology-Mycology, National Reference Center Echinococcosis, Besançon, France
Detry, Olivier ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chirurgie abdo, sénologique, endocrine et de transplantation
Hayette, Marie-Pierre ; Centre Hospitalier Universitaire de Liège - CHU > > Service de microbiologie clinique
Genetic diversity of Echinococcus multilocularis specimens isolated from Belgian patients with alveolar echinococcosis using EmsB microsatellites analysis.
Publication date :
20 November 2023
Journal title :
Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases
Auer, H., Relevance of parasitological examinations for the clinical course, epidemiology and prevention of alveolar echinococcosis - experiences of more than two decades in Austria. Wien. Klin. Wochenschr.118(19-20 3 (2006), 18–26.
Bart, J.M., Knapp, J., Gottstein, B., El-Garch, F., Giraudoux, P., Glowatzki, M.L., et al. EmsB, a tandem repeated multi-loci microsatellite, new tool to investigate the genetic diversity of Echinococcus multilocularis. Infect. Genet. Evol. 6:5 (2006 Sep), 390–400.
Baumann, S., Shi, R., Liu, W., Bao, H., Schmidberger, J., Kratzer, W., et al. Worldwide literature on epidemiology of human alveolar echinococcosis: a systematic review of research published in the twenty-first century. Infection 47:5 (2019 Oct), 703–727.
Casulli, A., Bart, J.M., Knapp, J., La Rosa, G., Dusher, G., Gottstein, B., et al. Multi-locus microsatellite analysis supports the hypothesis of an autochthonous focus of Echinococcus multilocularis in northern Italy. Int. J. Parasitol., 39(7), 2009 Jun 837-42.
Deplazes, P., Rinaldi, L., Alvarez Rojas, C.A., Torgerson, P.R., Harandi, M.F., Romig, T., et al. Global distribution of alveolar and cystic echinococcosis. Adv. Parasitol. 95 (2017), 315–493.
Dezsényi, B., Dubóczki, Z., Strausz, T., Csulak, E., Czoma, V., Káposztás, Z., et al. Emerging human alveolar echinococcosis in Hungary (2003–2018): a retrospective case series analysis from a multi-Centre study. BMC Infect. Dis., 21(1), 2021 Feb, 168.
Eroglu, F., Dokur, M., Ulu, Y., The molecular epidemiology of cystic and alveolar echinococcosis disease in Southeast Turkey. Iran. J. Parasitol. 16:2 (2021 Apr-Jun), 327–335.
Grenouillet, F., Chauchet, A., Richou, C., Vuitton, D.A., Knapp, J., Millon, L., et al. Alveolar echinococcosis: epidemiology, survey and management. J. des Anti-Infectieux. 15 (2013), 204–214.
Hanosset, R., Saegerman, C., Adant, S., Massart, L., Losson, B., Echinococcus multilocularis in Belgium: prevalence in red foxes (Vulpes vulpes) and in different species of potential intermediate hosts. Vet. Parasitol. 151:2–4 (2008 Feb), 212–217.
Jansen, F., Claes, M., Bakkers, E., Aryal, A., Madimba, K.C., Gabriël, S., et al. Echinococcus multilocularis in red foxes in North Belgium: prevalence and trends in distribution. Vet. Parasitol. Reg. Stud. Rep., 22, 2020 Dec, 100470.
Knapp, J., Bart, J.M., Glowatzki, M.L., Ito, A., Gerard, S., Maillard, S., et al. Assessment of use of microsatellite polymorphism analysis for improving spatial distribution tracking of Echinococcus multilocularis. J. Clin. Microbiol. 45:9 (2007 Sep), 2943–2950.
Knapp, J., Guislain, M.H., Bart, J.M., Raoul, F., Gottstein, B., Giraudoux, P., et al. Genetic diversity of Echinococcus multilocularis on a local scale. Infect. Genet. Evol., 2008.
Knapp, J., Bart, J.M., Giraudoux, P., Glowatzki, M.L., Breyer, I., Raoul, F., et al. Genetic-diversity of the cestode Echinococcus multilocularis in red foxes at a continental scale in Europe. PLoS Negl. Trop. Dis., 2009.
Knapp, J., Bart, J.M., Maillard, S., Gottstein, B., Piarroux, R., The genomic Echinococcus microsatellite EmsB sequences: from a molecular marker to the epidemiological tool. Parasitology. 137:3 (2010 Mar), 439–449.
Knapp, J., Damy, S., Brillaud, J., Tissot, J.D., Navion, J., Mélior, R., et al. EWET: data collection and interface for the genetic analysis of Echinococcus multilocularis based on EmsB microsatellite. PLoS One, 12(10), 2017 Oct, e0183849.
Knapp, J., Gottstein, B., Bretagne, S., Bart, J.M., Umhang, G., Richou, C., et al. Genotyping echinococcus multilocularis in human alveolar echinococcosis patients: an emsb microsatellite analysis. Pathogens, 9(4), 2020 Apr, 282.
Knapp, J., Meyer, A., Courquet, S., Millon, L., Raoul, F., Gottstein, B., et al. Echinococcus multilocularis genetic diversity in Swiss domestic pigs assessed by EmsB microsatellite analyzes. Vet. Parasitol., 2021.
Maillard, S., Gottstein, B., Haag, K.L., Ma, S., Colovic, I., Benchikh-Elfegoun, M.C., et al. The EmsB tandemly repeated multilocus microsatellite: a new tool to investigate genetic diversity of Echinococcus granulosus sensu lato. J. Clin. Microbiol. 47:11 (2009 Nov), 3608–3616.
Shang, J.Y., Zhang, G.J., Liao, S., Yu, W.J., He, W., Wang, Q., et al. Low genetic variation in Echinococcus multilocularis from the Western Sichuan plateau of China revealed by microsatellite and mitochondrial DNA markers. Acta Trop., 221, 2021 Sep, 105989.
Templeton, A.R., The reality and importance of founder speciation in evolution. BioEssays., 30, 2008.
Umhang, G., Knapp, J., Hormaz, V., Raoul, F., Boué, F., Using the genetics of Echinococcus multilocularis to trace the history of expansion from an endemic area. Infect. Genet. Evol., 2014.
Umhang, G., Karamon, J., Hormaz, V., Knapp, J., Cencek, T., Boué, F., A step forward in the understanding of the presence and expansion of Echinococcus multilocularis in Eastern Europe using microsatellite EmsB genotyping in Poland. Infect. Genet. Evol. 54 (2017 Oct), 176–182.
Umhang, G., Knapp, J., Wassermann, M., Bastid, V., Peytavin de Garam, C., Boué, F., et al. Asian admixture in European echinococcus multilocularis populations: new data from poland comparing EmsB microsatellite analyses and mitochondrial sequencing. Front. Vet. Sci., 2021.
Umhang, G., Bastid, V., Avcioglu, H., Bagrade, G., Bujanić, M., Bjelić Čabrilo, O., et al. Unravelling the genetic diversity and relatedness of Echinococcus multilocularis isolates in Eurasia using the EmsB microsatellite nuclear marker. Infect. Genet. Evol., 2021.
WHO, Fact Sheet no. 377. 2015 WHO | Echinococcosis.
Wilson, J.F., Rausch, R.L., McMahon, B.J., Schantz, P.M., Parasiticidal effect of chemotherapy in alveolar hydatid disease: review of experience with mebendazole and albendazole in alaskan eskimos. Clin. Infect. Dis. 15:2 (1992 Aug), 234–249.
