Article (Scientific journals)
Genetic diversity of Echinococcus multilocularis specimens isolated from Belgian patients with alveolar echinococcosis using EmsB microsatellites analysis.
Sacheli, Rosalie; Knapp, Jenny; PHOLIEN, Caroline et al.
2023In Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases, 116, p. 105531
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Keywords :
Belgium; Echinococcus multilocularis; EmsB; Human; Microsatellites; Microbiology; Ecology, Evolution, Behavior and Systematics; Molecular Biology; Genetics; Microbiology (medical); Infectious Diseases
Abstract :
[en] The genetic diversity of Echinococcus multilocularis (E. multilocularis) specimens isolated from patients with alveolar echinococcosis (AE), is a major field of investigation to correlate with sources of infection, clinical manifestations and prognosis of the disease. Molecular markers able to distinguish samples are commonly used worldwide, including the EmsB microsatellite. Here, we report the use of the EmsB microsatellite polymorphism data mining for the retrospective typing of Belgian specimens of E. multilocularis infecting humans. A total of 18 samples from 16 AE patients treated between 2006 and 2021 were analyzed through the EmsB polymorphism. Classification of specimens was performed through a dendrogram construction in order to compare the similarity among Belgian samples, some human referenced specimens on the EWET database (EmsB Website for the Echinococcus Typing) and previously published EmsB profiles from red foxes circulating in/near Belgium. According to a comparison with human European specimens previously genotyped in profiles, the 18 Belgian ones were classified into three EmsB profiles. Four specimens could not be assigned to an already known profile but some are near to EWET referenced samples. This study also highlights that some specimens share the same EmsB profile with profiles characterized in red foxes from north Belgium, the Netherlands, Luxembourg and French department near to the Belgian border. Furthermore, Belgian specimens present a genetic diversity and include one profile that don't share similarities with the ones referenced in the EWET database. However, at this geographical scale, there is no clear correlation between EmsB profiles and geographical location. Further studies including additional clinical samples and isolates from foxes and rodents of south Belgium are necessary to better understand the spatial and temporal circumstances of human infections but also a potential correlation between EmsB profiles and parasite virulence.
Disciplines :
Radiology, nuclear medicine & imaging
Author, co-author :
Sacheli, Rosalie  ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de microbiologie clinique
Knapp, Jenny;  University of Franche-Comté Laboratory Chrono-environment UMR CNRS 6249, Besançon, France, University Hospital of Besançon, Department of Parasitology-Mycology, National Reference Center Echinococcosis, Besançon, France
PHOLIEN, Caroline ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de microbiologie clinique
EGREK, Sabrina ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de microbiologie clinique
Léonard, Philippe ;  Centre Hospitalier Universitaire de Liège - CHU > > Service des maladies infectieuses - médecine interne
Giot, Jean-Baptiste ;  Centre Hospitalier Universitaire de Liège - CHU > > Service des maladies infectieuses - médecine interne
Delaere, Bénédicte;  EchinoLiege, University Hospital of Liege (CHU-ULg), Liege, Belgium, Catholic University of Louvain, CHU-UCL Namur, Department of infectiology, Yvoir, Belgium
BLETARD, Noëlla ;  Centre Hospitalier Universitaire de Liège - CHU > > Service d'anatomie et cytologie pathologiques
Gofflot, Stéphanie ;  Centre Hospitalier Universitaire de Liège - CHU > > Biothèque Hospitalo-Universitaire de Liège (BHUL)
Nollevaux, Marie-Cécile;  Catholic University of Louvain, CHU-UCL Namur, Department of Pathology, Yvoir, Belgium
Meunier, Paul ;  Centre Hospitalier Universitaire de Liège - CHU > > Service médical de radiodiagnostic
Lovinfosse, Pierre ;  Centre Hospitalier Universitaire de Liège - CHU > > Service médical de médecine nucléaire et imagerie onco
Pirotte, Benoit;  CHR Citadelle Hospital, Department of Infectious Diseases, Liege, Belgium
Truyens, Carine;  Free University of Brussels, Department of parasitology, Brussels, Belgium
Millon, Laurence;  University of Franche-Comté Laboratory Chrono-environment UMR CNRS 6249, Besançon, France, University Hospital of Besançon, Department of Parasitology-Mycology, National Reference Center Echinococcosis, Besançon, France
Detry, Olivier  ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de chirurgie abdo, sénologique, endocrine et de transplantation
Hayette, Marie-Pierre ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de microbiologie clinique
More authors (7 more) Less
Language :
English
Title :
Genetic diversity of Echinococcus multilocularis specimens isolated from Belgian patients with alveolar echinococcosis using EmsB microsatellites analysis.
Publication date :
20 November 2023
Journal title :
Infection, Genetics and Evolution: Journal of Molecular Epidemiology and Evolutionary Genetics of Infectious Diseases
ISSN :
1567-1348
eISSN :
1567-7257
Publisher :
Elsevier B.V., Netherlands
Volume :
116
Pages :
105531
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 19 January 2024

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