[en] Among the different analytical tools proposed as an alternative to the very expensive gas chromatography high-resolution mass spectrometry (GC-HRMS) analyses of polychlorodibenzo-p-dioxin and polychlorodibenzofurans, Chemically Activated LUciferase gene eXpression (CALUX) in vitro cell bioassay is very promising. It allows the analyses of a high number of samples since it is relatively fast, inexpensive, and sensitive. However, this technique is not yet widely applied for screening or environmental monitoring. The main reasons are probably the lack of validation and the difficulty in interpreting the global biological response of the bioassay. In this paper, the strict quality control criteria set up for the validation of CALUX are described. The validation has shown good repeatability (relative standard deviation (RSD) = 9%) and good within-lab reproducibility (RSD = 15%) of the results. The quantification limit, in the conditions applied in this paper, is 1.25 pg CALUX-TEQ/g fat. Comparison of CALUX and GC-HRMS analysis was made for various marine matrixes (fishes, mussels, starfishes, sea birds, and marine mammals). Good correlations are usually observed, but there are systematic differences between the results. Attempts are made to identify the origin of the discrepancy between the two methods.
Windal, Isabelle; Institut Scientifique de la Santé publique = Wetenschappelijk Instituut Volksgezondheid (Belgique) - ISP = WIV > Denrées alimentaires
Van Wouwe, Nathalie; Institut Scientifique de la Santé publique = Wetenschappelijk Instituut Volksgezondheid (Belgique) - ISP = WIV
Eppe, Gauthier ; Université de Liège - ULiège > Centre interfac. d'analyse des résidus en traces (CART)
Xhrouet, Céline ; Université de Liège - ULiège > Département de chimie (sciences) > Département de chimie (sciences)
Debacker, Virginie ; Université de Liège - ULiège > Département des sciences et gestion de l'environnement > Ecologie animale et écotoxicologie
Baeyens, Willy
De Pauw, Edwin ; Université de Liège - ULiège > Chimie physique, spectrométrie de masse > Centre interfac. d'analyse des résidus en traces (CART)
Goeyens, Léo; Vrije Universiteit Brussel - VUB
Language :
English
Title :
Validation and interpretation of CALUX as a tool for the estimation of dioxin-like activity in marine biological matrixes.
Publication date :
15 March 2005
Journal title :
Environmental Science and Technology
ISSN :
0013-936X
eISSN :
1520-5851
Publisher :
Amer Chemical Soc, Washington, United States - Washington
Volume :
39
Issue :
6
Pages :
1741-1748
Peer reviewed :
Peer Reviewed verified by ORBi
Funding text :
'Impulse Program in Marine Sciences supported by the Belgian State, the Prime's Minister Services, scientific, Technical and Cultural Affairs (MN/12/95).'
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