Article (Scientific journals)
Microgravity triggers ferroptosis and accelerates senescence in the MG-63 cell model of osteoblastic cells.
Garbacki, Nancy; Willems, Jérôme; Neutelings, Thibaut et al.
2023In NPJ Microgravity, 9 (1), p. 91
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Keywords :
Space and Planetary Science; Physics and Astronomy (miscellaneous); Agricultural and Biological Sciences (miscellaneous); Biochemistry, Genetics and Molecular Biology (miscellaneous); Materials Science (miscellaneous); Medicine (miscellaneous)
Abstract :
[en] In space, cells sustain strong modifications of their mechanical environment. Mechanosensitive molecules at the cell membrane regulate mechanotransduction pathways that induce adaptive responses through the regulation of gene expression, post-translational modifications, protein interactions or intracellular trafficking, among others. In the current study, human osteoblastic cells were cultured on the ISS in microgravity and at 1 g in a centrifuge, as onboard controls. RNAseq analyses showed that microgravity inhibits cell proliferation and DNA repair, stimulates inflammatory pathways and induces ferroptosis and senescence, two pathways related to ageing. Morphological hallmarks of senescence, such as reduced nuclear size and changes in chromatin architecture, proliferation marker distribution, tubulin acetylation and lysosomal transport were identified by immunofluorescence microscopy, reinforcing the hypothesis of induction of cell senescence in microgravity during space flight. These processes could be attributed, at least in part, to the regulation of YAP1 and its downstream effectors NUPR1 and CKAP2L.
Research center :
GIGA-LCTB - GIGA Cancer-Connective Tissue Biology - ULiège
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Garbacki, Nancy  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques
Willems, Jérôme ;  Université de Liège - ULiège > GIGA > GIGA Cancer - Connective Tissue Biology
Neutelings, Thibaut ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Laboratoire des tissus conjonctifs
Lambert, Charles ;  Université de Liège - ULiège > GIGA > GIGA Cancer - Connective Tissue Biology
Deroanne, Christophe ;  Université de Liège - ULiège > GIGA > GIGA Cancer - Connective Tissue Biology
Adrian, Astrid;  Airbus Defence and Space, GmbH, 88090, Immenstaad, Germany
Franz, Markus;  Airbus Defence and Space, GmbH, 88090, Immenstaad, Germany
Maurer, Matthias;  European Space Agency (ESA), European Astronaut Centre (EAC), 51147, Cologne, Germany
De Gieter, Philippe;  European Space Agency (ESA), 2201, AZ, Noordwijk, The Netherlands
Nusgens, Betty;  Laboratory of Connective Tissues Biology, GIGA-Cancer, University of Liège, 4000, Liège, Belgium
Colige, Alain ;  Université de Liège - ULiège > GIGA > GIGA Cancer - Connective Tissue Biology
Language :
English
Title :
Microgravity triggers ferroptosis and accelerates senescence in the MG-63 cell model of osteoblastic cells.
Publication date :
16 December 2023
Journal title :
NPJ Microgravity
eISSN :
2373-8065
Publisher :
Springer Science and Business Media LLC, United States
Volume :
9
Issue :
1
Pages :
91
Peer reviewed :
Peer Reviewed verified by ORBi
Name of the research project :
Cytoskeleton
Funders :
BELSPO - Federaal Wetenschapsbeleid [BE]
Funding text :
The authors thank the Belgian Federal Science Policy Office (BELSPO) for the provision of financial support in the framework of the PRODEX Programme of the European Space Agency (ESA) under contract number PEA 4000123688.
Available on ORBi :
since 17 January 2024

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