[en] [en] AIM: Our aim was to compare the prevalence and load of nine pathobionts in subgingival samples of healthy individuals and periodontitis patients from four different countries.
METHODS: Five hundred and seven subgingival biofilm samples were collected from healthy subjects and periodontitis patients in Belgium, Chile, Peru and Spain. The prevalence and load of Eubacterium brachy, Filifactor alocis, Fretibacterium fastidiosum, Porphyromonas endodontalis, Porphyromonas gingivalis, Selenomonas sputigena, Treponema denticola, Tannerella forsythia and Treponema socranskii were measured by quantitative PCR.
RESULTS: The association with periodontitis of all species, except for T. socranskii, was confirmed in all countries but Peru, where only P. endodontalis, P. gingivalis and T. denticola were found to be significantly associated. Moreover, most species showed higher loads at greater CAL and PPD, but not where there was BOP. Through Principal Component Analysis, samples showed clearly different distributions by diagnosis, despite observing a smaller separation in Peruvian samples.
CONCLUSIONS: Unlike prevalence, relative load was found to be a reliable variable to discriminate the association of the species with periodontitis. Based on this, F. alocis, P. endodontalis, P. gingivalis, T. denticola and T. forsythia may be biomarkers of disease in Belgium, Chile and Spain, due to their significantly higher abundance in periodontitis patients.
Research center :
d‐BRU - Dental Biomaterials Research Unit - ULiège [BE]
Disciplines :
Dentistry & oral medicine
Author, co-author :
Àlvarez, Gerard ; Department of Microbiology, DENTAID Research Center, Cerdanyola del Vallès, Spain
Arredondo, Alexandre ; Department of Microbiology, DENTAID Research Center, Cerdanyola del Vallès, Spain
Isabal, Sergio ; Department of Microbiology, DENTAID Research Center, Cerdanyola del Vallès, Spain
Teughels, Wim ; Department of Oral Health Sciences, KU Leuven, Belgium & Dentistry, University Hospitals Leuven, Leuven, Belgium
Laleman, Isabelle ; Centre Hospitalier Universitaire de Liège - CHU > > Service de parodontologie, chirurgie bucco-dentaire et chirurgie implantaire ; Department of Oral Health Sciences, KU Leuven, Belgium & Dentistry, University Hospitals Leuven, Leuven, Belgium
Contreras, María José; School of Dentistry, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile
Isbej, Lorena ; School of Dentistry, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile ; Pharmacology and Toxicology Programme, Faculty of Medicine, Pontificia Universidad Católica de Chile, Santiago, Chile
Huapaya, Enrique ; Department of Periodontology, School of Dentistry, Universidad Científica del Sur, Lima, Peru
Mendoza, Gerardo; Department of Periodontology, School of Dentistry, Universidad Científica del Sur, Lima, Peru ; Department of Periodontics, University of Pennsylvania, School of dental Medicine, Philadelphia, Pennsylvania, USA
Mor, Carolina ; Department of Periodontology, Universitat Internacional de Catalunya, Barcelona, Spain
Nart, José ; Department of Periodontology, Universitat Internacional de Catalunya, Barcelona, Spain
Blanc, Vanessa ; Department of Microbiology, DENTAID Research Center, Cerdanyola del Vallès, Spain
León, Rubén ; Department of Microbiology, DENTAID Research Center, Cerdanyola del Vallès, Spain
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