[en] In this work, we have prepared novel bio-inspired photoactive antibacterial polymer coatings on stainless steel (SS), which possess good mechanical and antibacterial properties. The formation of the photoactive antibacterial polymer coatings consists of the sequential deposition of three components on SS substrate (1) a catechol-based cationic glue P(mDOPA)-co-P(DMAEMA+) used as a universal primer, which facilitates the strong anchoring to SS; (2) a silver loaded (Pox(mDOPA)-Ag0/PAH) nanogel decorated with o-quinones applied to enhance the antibacterial properties of the coating and to permit the covalent grafting of the photosensitizer, and (3) an ethylene diamine derivative of protoporphyrin IX (PPIX-ED). Porphyrins are widely recognized for their antibacterial activity by producing reactive oxygen species when exposed to visible light. To estimate the deposition of the components on the SS substrate, SEM-EDX elemental mapping analysis was applied. Scratch test, nanoindentation, and accelerated property mapping (XPM) analysis were used to assess the mechanical properties of the coatings. The established antibacterial activity of the prepared photoactive polymer coatings on SS against Gram-positive B. subtilis and Gram-negative E. coli strains demonstrates their potential applications in medical and biomedical fields.
Research Center/Unit :
CESAM - Complex and Entangled Systems from Atoms to Materials - ULiège [BE] CERM - Center for Education and Research on Macromolecules - ULiège [BE]
Disciplines :
Materials science & engineering Chemistry
Author, co-author :
Bryaskova, Rayna ; University of Chemical Technology and Metallurgy - Sofia - Department of Polymer Engineering - Bulgaria
Philipova, Nikoleta; University of Chemical Technology and Metallurgy - Sofia - Department of Polymer Engineering - Bulgaria
Georgiev, Nikolai; Department of Organic Synthesis, University of Chemical Technology and Metallurgy, Sofia, Bulgaria
Ganchev, Damyan; University of Chemical Technology and Metallurgy - Sofia - Department of Machine Elements and Non-Metal Constructions - Bulgaria
Lalov, Ivo; Department of Biotechnology, University of Chemical Technology and Metallurgy, Sofia, Bulgaria
Detrembleur, Christophe ; University of Chemical Technology and Metallurgy - Sofia - Department of Biotechnology - Bulgaria
Language :
English
Title :
Bio-inspired antibacterial polymer coatings with included silver nanoparticles and porphyrin-based photosensitizer
Publication date :
11 June 2023
Journal title :
Journal of Polymer Research
ISSN :
1022-9760
eISSN :
1572-8935
Publisher :
Springer Science and Business Media B.V.
Volume :
30
Issue :
6
Pages :
1999
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
BNSF - Bulgarian National Science Fund [BG] F.R.S.-FNRS - Fund for Scientific Research [BE] EU - European Union [BE]
Funding text :
This work was supported by the National Science Fund of Bulgaria (project no. KP-06-H29/5). Christophe Detrembleur is Research Director by F.R.S.- FNRS and thanks FNRS for funding. We acknowledge the provided access to the scientific infrastructure of the laboratory of Micro and Nanomechanics of Mechatronic Systems, with financial support by Grant No BG05M2OP001-1.002-0011-C02, financed by the Science and Education for Smart Growth Operational Program (2014–2020) and co-financed by the European Union through the European Structural and Investment funds.
Sefton AM (2004) Encyclopedia of gastroenterology 740–743
Walker T, Canales M, Noimark S, Page K, Parkin I, Faull J, Bhatti M, Ciricet L (2017) A light-activated antimicrobial surface is active against bacterial, viral and fungal organisms. Sci Rep 7:15298 DOI: 10.1038/s41598-017-15565-5
Noimark S, Bovis M, MacRobert AJ, Correia A, Allan E, Wilson M, Parkin IP (2013) Photobactericidal polymers; the incorporation of crystal violet and nanogold into medical grade silicone. RSC Adv 3:18383–18394 DOI: 10.1039/c3ra42629g
Page K, Wilson M, Parkin IP (2009) Antimicrobial surfaces and their potential in reducing the role of the inanimate environment in the incidence of hospital-acquired infections. J Mater Chem 19:3819–3831 DOI: 10.1039/b818698g
Piccirillo C, Perni S, Gil-Thomas J, Prokopovich P, Wilson M, Pratten J, Parkin IP (2009) Antimicrobial activity of methylene blue and toluidine blue O covalently bound to a modified silicone polymer surface. J Mater Chem 19:6167–6171 DOI: 10.1039/b905495b
Decraene V, Pratten J, Wilson M (2008) Novel light-activated Antimicrobial Coatings are effective against surface-deposited Staphylococcus aureus. Curr Microbiol 57:269–273 DOI: 10.1007/s00284-008-9188-7
Naik AJT, Ismail S, Kay Ch, Wilson M, Parkin IP (2011) Antimicrobial activity of polyurethane embedded with methylene blue, toluidene blue and gold nanoparticles against Staphylococcus aureus, illuminated with white light. Mater Chem Phys 129:446–450 DOI: 10.1016/j.matchemphys.2011.04.040
Bovis MJ, Noimark S, Woodhams JH, Kay CWM, Weiner J, Peveler WJ, Correia A, Wilson M, Allan E, Parkin IP (2015) Photosensitisation studies of silicone polymer doped with methylene blue and nanogold for antimicrobial applications. RSC Adv 5:54830–54842 DOI: 10.1039/C5RA09045H
Noimark S, Dunnill CW, Kay CWM, Perni S, Prokopovich P, Ismail S, Wilson M, Parkin IP (2012) Incorporation of methylene blue and nanogold into polyvinyl chloride catheters; a new approach for light-activated disinfection of surfaces. J Mater Chem 22:15388–15396 DOI: 10.1039/c2jm31987j
Noimark S, Allan E, Parkin IP (2014) Light-activated antimicrobial surfaces with enhanced efficacy induced by a dark-activated mechanism. Chem Sci 5:2216–2223 DOI: 10.1039/C3SC53186D
Peveler WJ, Noimark S, Azawi HA, Hwang GB, Crick CR, Allan E, Edel JB, Ivanov AP, MacRobert AJ, Parkin IP (2018) Covalently attached antimicrobial surfaces using BODIPY: improving efficiency and effectiveness. ACS Appl Mater Interfaces 10:98–104 DOI: 10.1021/acsami.7b13273
Krzywiecki M, Pluczyk-Małek S, Powroznik P, Ślusarczyk C, Król-Molenda W, Smykała S, Kurek J, Koptoń P, Łapkowski M, Blacha-Grzechnik A (2021) Chemical and electronic structure characterization of electrochemically deposited nickel tetraamino-phthalocyanine: a step toward more efficient deposition techniques for organic electronics application. J Phys Chem C 125:13542–13550 DOI: 10.1021/acs.jpcc.1c01396
Gusev I, Ferreira M, Versace DL, Abbad-Andaloussi S, Pluczyk-Małek S, Erfurt K, Duda A, Data P, Blacha-Grzechnik A (2022) Electrochemically deposited zinc (tetraamino)phthalocyanine as a light-activated antimicrobial coating effective against S. Aureus. Materials 15:975–984 DOI: 10.3390/ma15030975
Bryaskova R, Philipova N, Georgiev N, Lalov I, Bojinov V, Detrembleur C (2021) Photoactive mussels inspired polymer coatings: Preparation and antibacterial activity. J Appl Polym Sci 138:50769 DOI: 10.1002/app.50769
Charlot A, Sciannaméa V, Lenoir S, Faure E, Jérôme R, Jérôme C, Van De Weerdt C, Martial J, Archambeau C, Willet N, Duwez AS (2009) All-in-one strategy for the fabrication of antimicrobial biomimetic films on stainless steel. Mater Chem 19:4117–4125 DOI: 10.1039/b820832h
Faure E, Lecomte P, Lenoir S, Vreuls C, Van De Weerdt C, Archambeau C, Martial J, Jérôme C, Duwez AS, Detrembleur C (2011) Sustainable and bio-inspired chemistry for robust antibacterial activity of stainless steel. J Mater Chem 21:7901–7904 DOI: 10.1039/c1jm11380a
Faure E, Falentin-Daudré C, Lanero TS, Vreuls C, Zocchi G, Van De Weerdt C, Martial J, Jérôme C, Duwez AS, Detrembleur C (2012) Functional nanogels as platforms for imparting antibacterial, antibiofilm, and antiadhesion activities to stainless steel. Adv Funct Mater 22:5271–5282 DOI: 10.1002/adfm.201201106
Faure E, Falentin-Daudré C, Jérôme C, Lyskawa J, Fournier D, Woisel P, Detrembleur C (2013) Catechols as versatile platforms in polymer chemistry. Prog Polym Sci 38:236–270 DOI: 10.1016/j.progpolymsci.2012.06.004
Patil N, Jerome C, Detrembleur C (2018) Recent advances in the synthesis of catechol-derived (bio) polymers for applications in energy storage and environment. Prog Polym Sci 82:34–91 DOI: 10.1016/j.progpolymsci.2018.04.002
Reis ER, Metze K, Nicola EMD, Nicola JH, Borissevitch IE (2013) Photodynamic activity of protoporphyrin IX in harderian glands of Wistar rats: monitoring by gland fluorescence. J Lumin 137:32–36 DOI: 10.1016/j.jlumin.2012.11.032
Javed F, Samaranayake LP, Romanos GE (2014) Treatment of oral fungal infections using antimicrobial photodynamic therapy: a systematic review of currently available evidence. Photochem Photobiol Sci 13:726–734 DOI: 10.1039/c3pp50426c
Vasilev K (2019) Nanoengineered antibacterial coatings and materials: a perspective. Coatings 9:654–666 DOI: 10.3390/coatings9100654
Lyutakov O, Hejna O, Solovyev A, Kalachyova Y, Svorcik V (2014) Polymethylmethacrylate doped with porphyrin and silver nanoparticles as light-activated antimicrobial material. RSC Adv 4:50624–50630 DOI: 10.1039/C4RA08385G
Philipova N, Georgiev N, Ganchev D, Lalov I, Bryaskova R (2023) Porhyrine/silver nanoparticles based photoactive antibacterial coatings. J Chem Technol Metall 58:275–282
Batakliev T, Ivanov E, Angelov V, Spinelli G, Kotsilkova R (2022) Advanced nanomechanical characterization of biopolymer films containing GNPs and MWCNTs in hybrid composite structure. Nanomaterials 12:709 DOI: 10.3390/nano12040709
Lei Z, Zhang L, Wie X (2008) One-step synthesis of silver nanoparticles by sonication or heating using amphiphilic block copolymer as templates. J Colloid Interface Sci 324:216–219 DOI: 10.1016/j.jcis.2008.04.050
Jennett NM, Owen-Jones S (2002) The scratch test: calibration, verification and the use of a certified reference material, NPL Measurement Good Practice Guide No. 54
Ahmadabadi N, Zadeh KK, Bhaskaran M, Sriram S (2013) In situ nanoindentation: probing nanoscale multifunctionality. Prog Mater Sci 58:1–29 DOI: 10.1016/j.pmatsci.2012.08.001
Němeček J, Lukeš J (2020) High-speed mechanical mapping of blended cement pastes and its comparison with standard ˘ modes of nanoindentation. Mater Today Commun 23:100806 DOI: 10.1016/j.mtcomm.2019.100806
Kim JS, Kuk E, Yu KN, Kim JH, Park SJ, Lee HJ (2007) Antimicrobial effects of silver nanoparticles. Nanomed Nanotechnol Biol Med 3:95–101 DOI: 10.1016/j.nano.2006.12.001