[en] [en] BACKGROUND: Autologous fat grafting is increasingly used worldwide and is a very attractive technique in many ways. However, treatment duration and postinjection tissue resorption remain problematic elements, which are largely related to the preparation method used. Moreover, few scientific studies objectively compare different fat preparation methods. This study analyzes the efficiency and quality of lipoaspirates prepared with a new filtration/centrifugation system (Adipure) in comparison with several existing techniques.
METHODS: Patient lipoaspirates were processed by five different techniques: decantation, centrifugation, Macrofill, Puregraft, and Adipure. Adipose tissue was evaluated in vitro for tissue resorption and oil formation, as well as in vivo after subcutaneous injections in immunodeficient mice. Adipose grafts were collected after 1 month, weighed, and analyzed by histology with a detailed scoring method.
RESULTS: Decanting gives inferior results to all other techniques, in terms of amount of tissue and oil in vitro, or graft weight and histological analysis in vivo. Methods using classical Coleman centrifugation (1200g), or a modified one (400g) associated with washes (Macrofill) produce very similar results, both in vitro and in vivo. Techniques using filtration systems (Puregraft and Adipure) produce less oil overall and have a higher grafting efficiency. The best results regarding grafting efficiency and oil quantity are found with the Adipure device.
CONCLUSIONS: A combination of filtration and very low-speed centrifugation potentiates the advantages of these techniques, in terms of graft efficiency. The adipose tissue purification being done in a few minutes, in an automatic way, undoubtedly provides a strong advantage for the use of this new system.
Disciplines :
Surgery
Author, co-author :
Nelissen, Xavier ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chirurgie plastique et maxillo-faciale
Licciardi, Séverine; Neosyad Company, Aix en Provence, France
Nizet, Christophe ; Centre Hospitalier Universitaire de Liège - CHU > > Service de chirurgie plastique et maxillo-faciale
Delay, Emmanuel; Department of Plastic and Reconstructive Surgery, Centre Léon Bérard, Lyon, France
Roche, Régis; Neosyad Company, Aix en Provence, France
Language :
English
Title :
Comparative Analysis of a New Automatic System and Four Existing Techniques for Autologous Fat Grafting.
Alternative titles :
[fr] Analyse comparative entre un nouveau système de lipofilling et 4 techniques existantes
Publication date :
October 2023
Journal title :
Plastic and Reconstructive Surgery, Global Open
eISSN :
2169-7574
Publisher :
Ovid Technologies (Wolters Kluwer Health), United States
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