Inorganic Chemistry; Organic Chemistry; Physical and Theoretical Chemistry; Computer Science Applications; Spectroscopy; Molecular Biology; General Medicine; Catalysis
Abstract :
[en] The castration of stallions is traditionally performed after puberty, at around the age of 2 years old. No studies have focused on the effects of early castration on osteoarticular metabolism. Thus, we aimed to compare early castration (3 days after birth) with traditional castration (18 months of age) in horses. Testosterone and estradiol levels were monitored from birth to 33 months in both groups. We quantified the levels of biomarkers of cartilage and bone anabolism (CPII and N-MID) and catabolism (CTX-I and CTX-II), as well as of osteoarthritis (HA and COMP) and inflammation (IL-6 and PGE2). We observed a lack of parallelism between testosterone and estradiol synthesis after birth and during puberty in both groups. The extra-gonadal synthesis of steroids was observed around the 28-month mark, regardless of the castration age. We found the expression of estrogen receptor (ESR1) in cartilage and bone, whereas androgen receptor (AR) expression appeared to be restricted to bone. Nevertheless, with respect to osteoarticular metabolism, steroid hormone deprivation resulting from early castration had no discernable impact on the levels of biomarkers related to bone and cartilage metabolism, nor on those associated with OA and inflammation. Consequently, our research demonstrated that early castration does not disrupt bone and cartilage homeostasis.
Disciplines :
Veterinary medicine & animal health Animal production & animal husbandry Anatomy (cytology, histology, embryology...) & physiology
Author, co-author :
Rouge, Marion ✱; Université de Caen-Normandie, OeReCa, 14000 Caen, France
Legendre, Florence ✱; Université de Caen Normandie BIOTARGEN, 14000 Caen, France
Elkhatib, Razan; Université de Caen-Normandie, OeReCa, 14000 Caen, France
Delalande, Christelle; Université de Caen-Normandie, OeReCa, 14000 Caen, France
Cognié, Juliette; INRAE, Université de Tours, Centre de Recherche de Tours, UMR PRC, 37380 Nouzilly, France
Reigner, Fabrice; INRAE, Université de Tours, Centre de Recherche de Tours, UEPAO, 37380 Nouzilly, France
Barrière, Philippe; INRAE, Université de Tours, Centre de Recherche de Tours, UEPAO, 37380 Nouzilly, France
Deleuze, Stefan ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée
Hanoux, Vincent; Université de Caen-Normandie, OeReCa, 14000 Caen, France
Galéra, Philippe ; Université de Caen Normandie BIOTARGEN, 14000 Caen, France
Bouraïma-Lelong, Hélène; Université de Caen-Normandie, OeReCa, 14000 Caen, France
✱ These authors have contributed equally to this work.
Language :
English
Title :
Early Castration in Horses Does Not Impact Osteoarticular Metabolism
Région normandie MESR - France. Ministère de l'Enseignement supérieur et de la Recherche Fonds Eperon
Funding text :
This work was supported by the Région Normandie, Institut Français du Cheval et de l’Equitation (IFCE), by the Ministère de l’Enseignement Supérieur, de la Recherche et de l’Innovation (Ministry of Higher Education and Research), by Fonds Eperon (EQUISTEM, N80-2014, 917CB194), and by GIS CENTAURE equine research (EQUISTEM-G,014CJ061). These funding sources had no involvement in the study design, collection, analysis and interpretation of data, in the writing of the manuscript, or in the decision to submit the manuscript for publication.
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