Restoration of A P657-647 form from P645-638 in extracts of etiolated primary bean leaves

[en] Measurements of absorption, fluorescence and fluorescence excitation spectra at liquid nitrogen temperature show that it is possible to restore in vitro a P657-647 protochlorophyllide lipoprotein complex (PLC) starting from preparations which essentially contain the P645-638 complex. The restoration is obtained by drying according to an experimental procedure which is described. Evidence for the attribution to the restored P657-647 of a Soret absorption band at 460 nm is given. © 1975.

Disciplines :

Phytobiology (plant sciences, forestry, mycology...)

Author, co-author :

Brouers, M.; Départment de Botanique, Laboratoire de Photobiologie, Université de Liège, 4000 Sart Tilman par Liège 1, Belgium

Sironval, Cyrille ; Université de Liège - ULiège

Language :

English

Title :

Restoration of A P657-647 form from P645-638 in extracts of etiolated primary bean leaves

Publication date :

1975

Journal title :

Plant Science Letters

ISSN :

0304-4211

Publisher :

Elsevier, Nl

Volume :

Issue :

Pages :

175 - 181

Funding text :

This work was supported by the research grant No. 735 from the "Fonds de la Recherche Fondamentale Collective" Bruxelles, Belgium. The authors are much indebted to Mrs. Piron-Fraipont for the preparation of the PLC precipitate, to Mrs. F. Hayet:Brouers for cultivating the leaf material and to Mr. F. Ohn and Mr. H. Wenig for technical assistance.

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since 30 November 2023

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Bibliography

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Schopfer, Siegelman (1968) Purification of Protochlorophyllide Holochrome. PLANT PHYSIOLOGY 43:990.
Henningsen, Kahn (1971) Photoactive Subunits of Protochlorophyll(ide) Holochrome. PLANT PHYSIOLOGY 47:685.
Sironval, Brouers, Michel, Kuiper Photosynthetica 1968, 2:268.
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Klein, Schiff (1972) The Correlated Appearance of Prolamellar Bodies, Protochlorophyll(ide) Species, and the Shibata Shift during Development of Bean Etioplasts in the Dark. PLANT PHYSIOLOGY 49:619.
Kahn, Nielsen Biochim. Biophys. Acta 1974, 333:409.