Brucella spp.; Ecuador; cattle; competitive ELISA (c-ELISA); risk factors; serological survey; Brucellaspp; Immunology and Allergy; Molecular Biology; Immunology and Microbiology (all); Microbiology (medical); Infectious Diseases; General Immunology and Microbiology
Abstract :
[en] Bovine brucellosis is a worldwide zoonotic contagious disease. According to World Animal Health Information System reports Ecuador has presented an increasing number of bovine brucellosis outbreaks in the continental territory over the past years (756 in 2018 versus 964 in 2021), generating economic losses for producers and causing a risk to public health. A cross-sectional study was conducted to investigate the seroprevalence of bovine brucellosis and associated risk or protective factors between May and June 2018. This stratified random study was implemented in 290 cattle herds located in the 23 provinces of continental Ecuador, which represents a total of 3737 cows aged 24 months or older. A competitive ELISA was used to detect Brucella antibodies. Simultaneously, an epidemiological survey was implemented to assess the brucellosis risk or protective factors. The apparent prevalence of bovine brucellosis at the herd level was 21.3% (95% CI: 16.8-26.6) and 6.2% (95% CI: 5.5-7) at the animal level. Univariate and multivariate logistic regression analyses were performed to determine the relationship between the potential factors associated with the presence of bovine brucellosis. The risk factors identified after multivariate analysis were a surface in ha per herd > 70 ha (OR = 2.73; 95% CI: 1.18-6.32) and the number of parturitions per animal (two or more with OR ≥ 1.8 and p-value ≤ 0.047). On the contrary, the protective factors were the region (farms located in the eastern region) and the absence of reported clinical signs. In addition, in herds where extensive production predominates, farmers have a low level of knowledge, and the farm biosecurity level is low. These results can guide the authorities in managing the risk factors identified, understanding the current epidemiological situation in Ecuador, improving the bovine brucellosis control program and food safety, as well as increase the one-health approach.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Garrido-Haro, Ana; Agencia de Regulación y Control Fito y Zoosanitario-AGROCALIDAD, Quito 170184, Ecuador ; Research Unit of Epidemiology and Risk Analysis Applied to Veterinary Science (UREAR-ULg), Fundamental and Applied Research for Animals & Health (FARAH) Center, Faculty of Veterinary Medicine, University of Liège, 4000 Liege, Belgium
Barrionuevo-Samaniego, Margoth; Agencia de Regulación y Control Fito y Zoosanitario-AGROCALIDAD, Quito 170184, Ecuador
Moreno-Caballeros, Paola; Agencia de Regulación y Control Fito y Zoosanitario-AGROCALIDAD, Quito 170184, Ecuador
Burbano-Enriquez, Alexandra; Agencia de Regulación y Control Fito y Zoosanitario-AGROCALIDAD, Quito 170184, Ecuador
Sánchez-Vázquez, Manuel J; Pan American Health Organization (PAHO), Pan American Center for Foot and Mouth Disease and Veterinary Public Health (PANAFTOSA), Rio de Janeiro 25020-000, Brazil
Pompei, Julio; Pan American Health Organization (PAHO), Pan American Center for Foot and Mouth Disease and Veterinary Public Health (PANAFTOSA), Rio de Janeiro 25020-000, Brazil
Humblet, Marie-France ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI)
Ron-Román, Jorge; Grupo de Investigación en Sanidad Animal y Humana (GISAH), Carrera Ingeniería Agropecuaria, Departamento de Ciencias de la Vida y la Agricultura, Universidad de las Fuerzas Armadas ESPE, Sangolquí 171103, Ecuador
Saegerman, Claude ; Université de Liège - ULiège > Département des maladies infectieuses et parasitaires (DMI) > Epidémiologie et analyse des risques appliqués aux sciences vétérinaires
Language :
English
Title :
Seroprevalence and Risk Factors Related to Bovine Brucellosis in Continental Ecuador.
Publication date :
05 September 2023
Journal title :
Pathogens
ISSN :
2076-0817
eISSN :
2076-0817
Publisher :
Multidisciplinary Digital Publishing Institute (MDPI), Switzerland
AGROCALIDAD - Agencia de Regulación y Control Fito y Zoosanitario ARES - Academy for Research and Higher Education
Funding text :
Our thanks to the Agencia de Regulación y Control Fito y Zoosanitario (AGROCALIDAD), the Ministry of Agriculture and Livestock of Ecuador (MAG), the Panamerican Center for Foot-and-Mouth Disease (PANAFTOSA) and the Belgian Development Cooperation (DGD), within the framework of the Institutional Collaboration in the Brucellosis and Trypanosomosis (Bru-Tryp) Project. Also, our thanks to the laboratory technicians, to the technicians who carried out the sampling at the national level, and to the AGROCALIDAD authorities who support the progress of Ecuador. Finally, this work would not have been possible without the help of the farmers, who collaborated and participated in this study for the benefit of the country and to whom we are grateful for their time and trust.This work was supported by the Agencia de Regulación y Control Fito y Zoosanitario (AGROCALIDAD), the National Brucellosis Program, the Ministry of Agriculture and Livestock of Ecuador (MAG), the Panamerican Centre for Foot-and-Mouth Disease (PANAFTOSA), and the Academy of Research and Higher Education (ARES, Belgium; https://www.ares-ac.be/en/cooperation-au-developpement ; accessed on 1 September 2023) through the Research for Development Project entitled “Establishment of a Platform to support training and awareness, diagnosis and development of a control strategy for brucellosis and trypanosomiasis in Ecuador” (acronym: BruTryp).
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