Desaturase; Functional characterisation; Heterologous expression; Integrated pest management; Lepidoptera; Transcriptome; Insect Science; Agronomy and Crop Science; General Medicine
Abstract :
[en] [en] BACKGROUND: The sugarcane borer Diatraea saccharalis (Lepidoptera) is a key pest on sugarcane and other grasses in the Americas. Biological control as well as insecticide treatments are used for pest management, but economic losses are still significant. The use of female sex pheromones for mating disruption or mass trapping in pest management could be established for this species, provided that economical production of pheromone is available.
RESULTS: Combining in vivo labelling studies, differential expression analysis of transcriptome data and functional characterisation of insect genes in a yeast expression system, we reveal the biosynthetic pathway and identify the desaturase and reductase enzymes involved in the biosynthesis of the main pheromone component (9Z,11E)-hexadecadienal, and minor components hexadecanal, (9Z)-hexadecenal and (11Z)-hexadecenal. We next demonstrate heterologous production of the corresponding alcohols of the pheromone components, by expressing multiple steps of the biosynthetic pathway in yeast.
CONCLUSION: Elucidation of the genetic basis of sex pheromone biosynthesis in D. saccharalis, and heterologous expression in yeast, paves the way for biotechnological production of the pheromone compounds needed for pheromone-based pest management of this species.
Disciplines :
Entomology & pest control
Author, co-author :
Dam, Marie Inger ; Department of Biology, Lund University, Sölvegatan 37, SE-223 62, Lund, Sweden
Ding, Bao-Jian; Department of Biology, Lund University, Sölvegatan 37, SE-223 62, Lund, Sweden
Svensson, Glenn P; Department of Biology, Lund University, Sölvegatan 37, SE-223 62, Lund, Sweden
Wang, Hong-Lei; Department of Biology, Lund University, Sölvegatan 37, SE-223 62, Lund, Sweden
Melo, Douglas J; Department of Biology, Lund University, Sölvegatan 37, SE-223 62, Lund, Sweden ; Departamento de Química, Universidade Federal do Paraná, CP 19081, 81531-990, Curitiba, PR, Brazil
Lassance, Jean-Marc ; Université de Liège - ULiège > Département de gestion vétérinaire des Ressources Animales (DRA) > Génomique animale
Zarbin, Paulo H G; Departamento de Química, Universidade Federal do Paraná, CP 19081, 81531-990, Curitiba, PR, Brazil
Löfstedt, Christer; Department of Biology, Lund University, Sölvegatan 37, SE-223 62, Lund, Sweden
Language :
English
Title :
Sex pheromone biosynthesis in the sugarcane borer Diatraea saccharalis: paving the way for biotechnological production.
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