[en] Until 1993, chlordecone (CLD) was extensively used in banana fields in French West Indies. In a previous study, CLD was detected in 90 % of Martinican and Guadeloupean adult's serum. In order to simplify the analyses of CLD in the serum, a new QuEChERS-HPLC-MS/MS method was implemented and validated by the Pasteur Institute of Guadeloupe (IPG). This method was validated with accuracy profiles according to the French Standard NF V03-110 plus the ISO 15189 and European guidelines. Linearity, repeatability, accuracy, intermediate precision, specificity, limit of detection (LOD), limit of quantification (LOQ) and uncertainty were determined. The accuracy profile allowed the method to be validated between 0.06 µg L-1 and 1.00 µg L-1 of serum. The LOD was 0.02 µg L-1, the LOQ was 0.06 µg L-1 and the uncertainty of the method was 21 %. A comparison of 49 serum samples between the IPG (LC-MS/MS) and the LEAE-CART (GC-HRMS) laboratories demonstrated that this new method can reliably determine CLD in human serum. Stability tests were performed and duration of the storage of raw samples and extracts before analysis by HPLC-MS/MS. Raw samples were stable after collection for at least one week at 5 °C or 25 °C and for at least 3 months at -20 °C. Extracts in acetonitrile were stable for at least 1 month at -20 °C. These stability results facilitate the daily use of the method. This method should help the entire population of Guadeloupe and Martinique by allowing a routinely analyzed for CLD and will be useful for future projects aimed at improving population health monitoring.
Disciplines :
Chemistry
Author, co-author :
Saint-Hilaire, Maïlie; Institut Pasteur Guadeloupe, Morne Joliviere, 97139 Les Abymes, Guadeloupe, France. Electronic address: msaint-hilaire@pasteur-guadeloupe.fr
Plumain, Didier; Institut Pasteur Guadeloupe, Morne Joliviere, 97139 Les Abymes, Guadeloupe, France
Thomé, Jean-Pierre ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Ecologie animale et écotoxicologie
Adam, Catherine ; Université de Liège - ULiège > Département de Biologie, Ecologie et Evolution > Ecologie animale et écotoxicologie
Scholl, Georges ; Université de Liège - ULiège > Département de chimie (sciences) > Center for Analytical Research and Technology (CART)
Vedy, Serge; Institut Pasteur Guadeloupe, Morne Joliviere, 97139 Les Abymes, Guadeloupe, France
Ferdinand, Séverine; Institut Pasteur Guadeloupe, Morne Joliviere, 97139 Les Abymes, Guadeloupe, France
Talarmin, Antoine; Institut Pasteur Guadeloupe, Morne Joliviere, 97139 Les Abymes, Guadeloupe, France
Guyomard-Rabenirina, Stéphanie; Institut Pasteur Guadeloupe, Morne Joliviere, 97139 Les Abymes, Guadeloupe, France
Language :
English
Title :
Validation of an HPLC-MS/MS method with QuEChERS extraction using isotopic dilution to analyze chlordecone in human serum.
Publication date :
28 September 2023
Journal title :
Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences
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