[en] The objective of this retrospective study was to evaluate the clinical significance of fecal quantitative real-time polymerase chain reaction (qPCR) Salmonella results when taking the cycle threshold values (Ct) into account. The study included 120 Salmonella qPCR-positive fecal samples obtained from 88 hospitalized horses over a 2-year period. The mean Ct of the qPCR test was evaluated in regard to (1) clinical outcome and (2) systemic inflammatory response syndrome (SIRS) status (no SIRS, moderate SIRS, or severe SIRS) of the sampled horses. An ROC analysis was performed to establish the optimal cut-off Ct values associated with severe SIRS. The mean ± SD Ct value was significantly lower in samples (1) from horses with a fatal issue (27.87 ± 5.15 cycles) than in surviving horses (31.75 ± 3.60 cycles), and (2) from horses with severe SIRS (27.87 ± 2.78 cycles) than from horses with no (32.51 ± 3.59 cycles) or moderate (31.54 ± 3.02 cycles) SIRS. In the ROC analysis, the optimal cut-off value of Ct associated with a severe SIRS was 30.40 cycles, with an AUC value of 0.84 [95% confidence interval 0.76-0.91] and an OR of 0.64 [0.51-0.79]. Results suggest that including the Ct value in the interpretation of fecal qPCR results could improve the diagnostic value of this test for clinical salmonellosis in horses.
Disciplines :
Veterinary medicine & animal health
Author, co-author :
Amory, Hélène ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée
Cesarini Latorre, Carlota ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée
De Maré, Lorie ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée
Loublier, Clémence ; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH) > FARAH: Médecine vétérinaire comparée
Moula, Nassim ; Université de Liège - ULiège > Département des sciences biomédicales et précliniques > Méthodes expérimentales des animaux de laboratoire et éthique en expérimentation animale
Detilleux, Johann ; Université de Liège - ULiège > Département de gestion vétérinaire des Ressources Animales (DRA)
Saulmont, Marc; Regional Animal Health and Identification Association (ARSIA), 2 Allée des Artisans, ZA du Biron, 5590 Ciney, Belgium
Garigliany, Mutien-Marie ✱; Université de Liège - ULiège > Département de morphologie et pathologie (DMP) > Pathologie générale et autopsies
Lecoq, Laureline ✱; Université de Liège - ULiège > Fundamental and Applied Research for Animals and Health (FARAH)
✱ These authors have contributed equally to this work.
Language :
English
Title :
Relationship between the Cycle Threshold Value (Ct) of a Salmonella spp. qPCR Performed on Feces and Clinical Signs and Outcome in Horses.
Alternative titles :
[fr] Relation entre la valeur seuil du cycle (Ct) d'une qPCR de Salmonella spp. effectuée sur les matières fécales et les signes cliniques et l'issue chez les chevaux.
Original title :
[en] Relationship between the Cycle Threshold Value (Ct) of a Salmonella spp. qPCR Performed on Feces and Clinical Signs and Outcome in Horses.
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