Inhibition of bacterial biofilms by the snake venom proteome.

[en] Snake venoms possess a range of pharmacological and toxicological activities. Here we evaluated the antibacterial and anti-biofilm activity against methicillin-susceptible and methicillin-resistant Staphylococcus aureus (MSSA and MRSA) of venoms from the Samar spitting cobra Naja samarensis and the Puff adder Bitis arietans. Both venoms prevented biofilm production by pathogenic S. aureus in a growth-independent manner, with the B. arietans venom being most potent. Fractionation showed the active molecule to be heat-labile and >10 kDa in size. Proteomic profiles of N. samarensis venom revealed neurotoxins and cytotoxins, as well as an abundance of serine proteases and three-finger toxins, while serine proteases, metalloproteinases and C-lectin types were abundant in B. arietans venom. These enzymes may have evolved to prevent bacteria colonising the snake venom gland. From a biomedical biotechnology perspective, they have valuable potential for anti-virulence therapy to fight antibiotic resistant microbes.

Disciplines :

Chemistry

Author, co-author :

Khan, Neyaz A; Pathogenic Mechanisms Research Group, School of Natural Sciences, University of Galway, Ireland

Gobbi Amorim, Fernanda ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.) ; Université de Liège - ULiège > Molecular Systems (MolSys)

Dunbar, John P; Venom Systems & Proteomics Lab, School of Natural Sciences, Ryan Institute, University of Galway, Ireland

Leonard, Dayle; Pathogenic Mechanisms Research Group, School of Natural Sciences, University of Galway, Ireland ; Venom Systems & Proteomics Lab, School of Natural Sciences, Ryan Institute, University of Galway, Ireland

Redureau, Damien ; Université de Liège - ULiège > Molecular Systems (MolSys) ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)

Quinton, Loïc ; Université de Liège - ULiège > Molecular Systems (MolSys) ; Université de Liège - ULiège > Département de chimie (sciences) > Laboratoire de spectrométrie de masse (L.S.M.)

Dugon, Michel M; Venom Systems & Proteomics Lab, School of Natural Sciences, Ryan Institute, University of Galway, Ireland

Boyd, Aoife ; Pathogenic Mechanisms Research Group, School of Natural Sciences, University of Galway, Ireland

Language :

English

Title :

Inhibition of bacterial biofilms by the snake venom proteome.

Publication date :

September 2023

Journal title :

Biotechnology Reports

eISSN :

2215-017X

Publisher :

Elsevier BV, Netherlands

Volume :

Pages :

e00810

Peer reviewed :

Peer reviewed

Additional URL :

https://api.elsevier.com/content/article/PII:S2215017X23000309?httpAccept=text/xml

Available on ORBi :

since 24 September 2023

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