Dermatophytes; Host shift; MAT idiomorph distribution; Phylogeny; Physicochemical features; Tinea capitis; Ecology, Evolution, Behavior and Systematics; Agricultural and Biological Sciences (miscellaneous)
Abstract :
[en] The Microsporum canis complex consists of one zoophilic species, M. canis, and two anthropophilic species, M. audouinii and M. ferrugineum. These species are the most widespread zoonotic pathogens causing dermatophytosis in cats and humans worldwide. To clarify the evolutionary relationship between the three species and explore the potential host shift process, this study used phylogenetic analysis, population structure analysis, multispecies coalescent analyses, determination of MAT idiomorph distribution, sexual crosses, and macromorphology and physicochemical features to address the above questions. The complex of Microsporum canis, M. audouinii and M. ferrugineum comprises 12 genotypes. MAT1-1 was present only in M. canis, while the anthropophilic entities contained MAT1-2. The pseudocleistothecia were yielded by the mating behaviour of M. canis and M. audouinii. Growth rates and lipase, keratinolysis and urea hydrolytic capacities of zoophilic M. canis isolates were all higher than those of anthropophilic strains; DNase activity of M. ferrugineum exceeded that of M. canis. The optimum growth temperature was 28 °C, but 22 °C favoured the development of macroconidia. Molecular data, physicochemical properties and phenotypes suggest the adaptation of zoophilic M. canis to anthropophilic M. ferrugineum, with M. audouinii in an intermediate position.
Disciplines :
Microbiology
Author, co-author :
Zhou, Xin ; Center of Expertise in Mycology of Radboud University Medical Center, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands ; Department of Dermatology, 3rd Affiliated Hospital, Sun Yat-senen University, Guangzhou, China
Ahmed, Sarah A ; Center of Expertise in Mycology of Radboud University Medical Center, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands ; Foundation Atlas of Clinical Fungi, Hilversum, The Netherlands
Tang, Chao; Center of Expertise in Mycology of Radboud University Medical Center, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands
Grisolia, Maria Eduarda ; Bioprocess Engineering and Biotechnology, Federal University of Paraná, Curitiba, Brazil
Warth, José Francisco Ghignatti; Microbiology, Parasitology and Pathology Graduate Program, Federal University of Paraná, Curitiba, Brazil
Webster, Kristen; Department of Dermatology, Center for Medical Mycology, University Hospitals, Cleveland, USA
Peano, Andrea; Department of Veterinary Sciences, University of Turin, Grugliasco, Italy
Cafarchia, Claudia ; Department of Veterinary Medicine, University of Bari Aldo Moro, Bari, Italy
Hayette, Marie-Pierre ; Centre Hospitalier Universitaire de Liège - CHU > > Service de microbiologie clinique
Sacheli, Rosalie ; Centre Hospitalier Universitaire de Liège - CHU > > Service de microbiologie clinique
Matos, Tadeja; Medical Faculty, Institute of Microbiology and Immunology, University of Ljubljana, Ljubljana, Slovenia
Kang, Yingqian; Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education of Guizhou & Guizhou Talent Base for Microbiology and Human Health, School of Basic Medical Sciences, Key Laboratory of Microbiology and Parasitology of Education Department of Guizhou, Guizhou Medical University, Guiyang, China. 449164105@qq.com ; Guizhou Provincial Academician Workstation of Microbiology and Health, Guizhou Academy of Tobacco Science, Guiyang, China. 449164105@qq.com
de Hoog, G Sybren ; Center of Expertise in Mycology of Radboud University Medical Center, Canisius Wilhelmina Hospital, Nijmegen, The Netherlands. sybren.dehoog@radboudumc.nl ; Foundation Atlas of Clinical Fungi, Hilversum, The Netherlands. sybren.dehoog@radboudumc.nl ; Bioprocess Engineering and Biotechnology, Federal University of Paraná, Curitiba, Brazil. sybren.dehoog@radboudumc.nl ; Key Laboratory of Environmental Pollution Monitoring and Disease Control, Ministry of Education of Guizhou & Guizhou Talent Base for Microbiology and Human Health, School of Basic Medical Sciences, Key Laboratory of Microbiology and Parasitology of Education Department of Guizhou, Guizhou Medical University, Guiyang, China. sybren.dehoog@radboudumc.nl
Feng, Peiying ; Department of Dermatology, 3rd Affiliated Hospital, Sun Yat-senen University, Guangzhou, China. fengpeiying77@163.com
CSC - China Scholarship Council [CN] CAPES - Coordenação de Aperfeicoamento de Pessoal de Nível Superior [BR]
Funding text :
This project was supported by the Natural Science Foundation of Guangdong Province of China (No.2020A1515011183). XZ was supported by China Scholarship Council (No.202006380049). MEG was supported by CAPES-PrInt (No.88887.311835/2018-00). The funders had no role in study design, data collection and analysis, publication decision, or manuscript preparation. This work also was supported by the 111 Project (D20009); China-Ukraine Intergovernmental Exchange Project (8); National Natural Science Foundation of China (NSFC; no. 32060034); International Science and Technology Cooperation Base of Guizhou Province ([2020]4101); Talent Base Project of Guizhou Province, China [RCJD2018-22]; High-level Innovation Talent Project of Guizhou Province(GCC[2022]036-1); Major Science and Technology Projects of China Tobacco [No.110202101048(LS-08)]; Foundation of Key Laboratory of Microbiology and Parasitology of Education Department, Guizhou (QJJ [2022] 019); Ministry of Education Project(07150120711).
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