Article (Scientific journals)
Generation of a Well-Characterized Homozygous Chromodomain-Helicase-DNA-Binding Protein 4G1003D Mutant hESC Line Using CRISPR/eCas9 (ULIEGEe001-A-1)
Chohra, Ilyas; Giri, Subhajit; Malgrange, Brigitte
2023In International Journal of Molecular Sciences, 24 (13), p. 10543
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Keywords :
Molecular Biology; crispr; human embryonic stem cells
Abstract :
[en] The chromatin remodeler Chromodomain-helicase-DNA-binding protein 4 (CHD4) is crucial for the development of multiple organ systems. Functional mutations of CHD4 have recently been described in a developmental disorder, namely Siffrim-Hitz-Weiss syndrome (SIHIWES). Herein, we have generated a homozygous CHD4G1003D hESC line (WAe025-A-1) using CRISPR/eCas9-based gene editing in the WA-25 hESC line. The edited hESC line maintains normal karyotype, pluripotency, and ability to differentiate into three germ layers. This cell line will be a valuable resource for studying the functional role of CHD4 during the development and disease modeling of SIHIWES in vitro.
Research center :
GIGA Stem Cells-Developmental Neurobiology - ULiège
Disciplines :
Otolaryngology
Biochemistry, biophysics & molecular biology
Author, co-author :
Chohra, Ilyas ;  Université de Liège - ULiège > GIGA > GIGA Stem Cells - Developmental Neurobiology ; Developmental Neurobiology Unit, GIGA-Stem Cells, Av Hippocrate, 15 B-4000 Liege, Belgium
Giri, Subhajit  ;  Université de Liège - ULiège > Département des sciences cliniques > Neurologie ; Developmental Neurobiology Unit, GIGA-Stem Cells, Av Hippocrate, 15 B-4000 Liege, Belgium
Malgrange, Brigitte  ;  Université de Liège - ULiège > Département des sciences cliniques > Neurologie ; Developmental Neurobiology Unit, GIGA-Stem Cells, Av Hippocrate, 15 B-4000 Liege, Belgium
Language :
English
Title :
Generation of a Well-Characterized Homozygous Chromodomain-Helicase-DNA-Binding Protein 4G1003D Mutant hESC Line Using CRISPR/eCas9 (ULIEGEe001-A-1)
Publication date :
23 June 2023
Journal title :
International Journal of Molecular Sciences
ISSN :
1661-6596
eISSN :
1422-0067
Publisher :
MDPI AG
Volume :
24
Issue :
13
Pages :
10543
Peer reviewed :
Peer Reviewed verified by ORBi
Funders :
F.R.S.-FNRS - Fund for Scientific Research [BE]
Fonds Léon Fredericq [BE]
ULiège - University of Liège [BE]
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since 31 July 2023

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