Article (Scientific journals)
Multicenter Performance Evaluation of the Revogene® GBS DS Real-Time PCR Assay for Group B Streptococcus Detection During Labor.
d'Otreppe, Stéphanie; Lefèvre, Philippe; Meex, Cécile et al.
2023In Molecular Diagnosis and Therapy
Peer Reviewed verified by ORBi
 

Files


Full Text
Multicenter Performance Evaluation of the Revogene® GBS DS Real‐Time PCR Assay for Group B Streptococcus Detection During Labor 2023.pdf
Author postprint (806.86 kB)
Request a copy

All documents in ORBi are protected by a user license.

Send to



Details



Keywords :
Group B Streptococcus; antenatal screening; intrapartum antibiotic prophy- laxis; Revogene® GBS DS assay; point-of-care test
Abstract :
[en] [en] PURPOSE: This study aimed to evaluate the performance and ease of use of the Revogene® GBS DS PCR assay for the intrapartum detection of Group B Streptococcus (GBS) colonization, as compared with intrapartum culture and antenatal culture-based screening. METHODS: Between April and August 2019, 398 women who gave birth in one of the three maternities participating in this study agreed to the collection of a vaginal swab when they arrived in the labor ward. The samples were immediately sent to the adjacent laboratory where they were discharged into the buffer provided with the Revogene® GBS DS assay. Part of the buffer was used to perform the Revogene® GBS DS test, and part of the same buffer was used for GBS culture. RESULTS: The Revogene® GBS DS assay provided a valid result in less than 70 min for 356 (89%) women. The sensitivity of the test was 85.7% (66.4-95.3%). The specificity of the test was 99.1% (97.3-99.8%). The positive predictive value was 88.9% (69.7-97.1%). The negative predictive value was 98.9% (96.9-99.6%). CONCLUSION: The easy-to-use Revogene® GBS DS assay provides a valuable tool for the detection of GBS colonization at the beginning of labor. The sensitivity and turn-around time are adequate. The high number of invalid results needs to be addressed before the Revogene® GBS DS test can be expected to replace the current screening-based approach.
Disciplines :
Laboratory medicine & medical technology
Reproductive medicine (gynecology, andrology, obstetrics)
Immunology & infectious disease
Public health, health care sciences & services
Author, co-author :
d'Otreppe, Stéphanie ;  Department of Clinical Biology, Hôpital de Marche, Vivalia, Marche-en-Famenne, Belgium. stephanie.d'otreppe@lhub-ulb.be
Lefèvre, Philippe;  Department of Clinical Biology, Hôpital de Marche, Vivalia, Marche-en-Famenne, Belgium
Meex, Cécile  ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de microbiologie clinique
DEVEY, Anaïs  ;  Centre Hospitalier Universitaire de Liège - CHU > > Service d'hématologie biologique et immuno-hématologie ; Department of Clinical Biology, Hôpital de Libramont, Vivalia, Libramont-Chevigny, Belgium
Sacheli, Rosalie  ;  Centre Hospitalier Universitaire de Liège - CHU > > Service de microbiologie clinique
Gerard, Martin ;  Université de Liège - ULiège > Département des sciences cliniques > Gynécologie-Obstétrique ; Department of Obstetrics and Gynecology, Hôpital de Marche, Vivalia, Marche-en-Famenne, Belgium
Melin, Pierrette  ;  Université de Liège - ULiège > Département des sciences biomédicales et précliniques
Language :
English
Title :
Multicenter Performance Evaluation of the Revogene® GBS DS Real-Time PCR Assay for Group B Streptococcus Detection During Labor.
Publication date :
20 July 2023
Journal title :
Molecular Diagnosis and Therapy
ISSN :
1177-1062
Publisher :
Springer Science and Business Media LLC, New Zealand
Peer reviewed :
Peer Reviewed verified by ORBi
Available on ORBi :
since 27 July 2023

Statistics


Number of views
32 (5 by ULiège)
Number of downloads
2 (0 by ULiège)

Scopus citations®
 
0
Scopus citations®
without self-citations
0
OpenCitations
 
0

Bibliography


Similar publications



Contact ORBi