[en] The accurate chemical structure of the precursor of chlorophyll, protochlorophyllide which has been accumulated in etiolated primary bean leaves has not yet been completely assessed*,especially with respect to the occurence of an ester group (phytol, methyl...) at the propionic chain, or of a second vinyl at carbon C4. As absorption and fluorescence spectroscopy is unable to solve this question we have recorded the high resolution (360 MHz) 1HMR spectra of the purified pigment extracted from etiolated bean leaves. For valuable comparison, the esterified pigment (protochlorophyll = PChl) from the pumpkin cuticle has been extracted and purified. This pigment is esterified by a long-chain alcohol.
Martinus Nijhoff/Dr W. Junk Publishers, The Hague/Boston/Lancaster, Netherlands
ISBN/EAN :
90-247-2946-7
Collection name :
Advances in Agricultural Biotechnology; Vol. IV
Pages :
753-756
Peer reviewed :
Editorial reviewed
Funders :
F.R.S.-FNRS - Fonds de la Recherche Scientifique
Funding number :
80/85-1983
Funding text :
We thank the 'Fonds National de la Recherche Scientifique Belge' and the 'Actions Concertées nº 80/85-1983' for financial support. We thank Professor Anteunis and Dr Borremans from the University of Ghent for the access to the Bruker WH 360 NMR spectrometer.
