[en] The high mortality upon enteric virus infection in piglets causes huge economic losses. To control these infections, potential causes for this high susceptibility for enteric virus infections in younger piglets were analyzed by comparing the intestinal barrier between 1-week, 2-week and 4-week-old piglets. In this study, histological staining was used to analyze morphological differences in intestinal villi, real-time qPCR was performed to assess mRNA expression levels of genes that were related to viral infection and differentiation of immune cells, and flow cytometry was utilized to measure the frequencies of T cells. According to the results obtained, 1-week-old piglets have intestinal villi with shallower crypts, less well developed epithelial cells and a more immature immune system compared to older pigs. Moreover, high amounts of enteric virus invasion-assisting proteins but low amounts of resistant proteins in 1-week piglets could also be a reason for the high susceptibility of 1-week-old piglets.
Disciplines :
Biochemistry, biophysics & molecular biology
Author, co-author :
Yang, Shanshan ✱; State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, China, Cell Biology and Immunology Group, Wageningen University and Research, Wageningen, the Netherlands
Yang, Ning ✱; Université de Liège - ULiège > TERRA Research Centre
Huang, Xin; State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, China
Li, Yang; State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, China, Molecular and Cellular Epigenetics (GIGA) and Molecular Biology (TERRA), University of Liege, Belgium
Liu, Guo; State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, China
Jansen, Christine A; Cell Biology and Immunology Group, Wageningen University and Research, Wageningen, the Netherlands
Savelkoul, Huub F J; Cell Biology and Immunology Group, Wageningen University and Research, Wageningen, the Netherlands
Liu, Guangliang ; State Key Laboratory of Veterinary Etiological Biology, College of Veterinary Medicine, Lanzhou University, Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou, Gansu, 730046, China. Electronic address: LiuGuangliang01@caas.cn
✱ These authors have contributed equally to this work.
Language :
English
Title :
Pigs' intestinal barrier function is more refined with aging.
This work was supported by the National Natural Science Foundation of China (31972689), WUR-CAAS joint Ph.D. Program and ULg-CAAS joint Ph.D. Program.This work was supported by the National Natural Science Foundation of China ( 31972689 ), WUR-CAAS joint Ph.D. Program and ULg-CAAS joint Ph.D. Program.
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