Identification of Fcl-29 as an Effective Antifungal Natural Product against Fusarium graminearum and Combinatorial Engineering Strategy for Improving Its Yield.

Li, Beibei

doi:10.1021/acs.jafc.2c09012

Article (Scientific journals)

Identification of Fcl-29 as an Effective Antifungal Natural Product against Fusarium graminearum and Combinatorial Engineering Strategy for Improving Its Yield.

Li, Beibei

2023 • In Journal of Agricultural and Food Chemistry, 71 (14), p. 5554 - 5564

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https://hdl.handle.net/2268/304445

DOI
10.1021/acs.jafc.2c09012

PubMed
36995163

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Keywords :

Fusarium graminearum; Xenorhabdus budapestensis; fabclavine; fermentation; genetic engineering; Antifungal Agents; Plant Diseases/microbiology; Antifungals; Fusarium head blights; Natural products; Plant Diseases; Agricultural and Biological Sciences (all); General Agricultural and Biological Sciences

Abstract :

[en] Fusarium head blight (FHB), caused by Fusarium graminearum, whose occurrence and prevalence causes 10-70% wheat production loss, is one of the most destructive diseases influencing the production of wheat globally. To identify the potential natural products (NPs) against F. graminearum, we screened 59 Xenorhabdus strains and discovered that the cell-free supernatant (CFS) of X. budapestensis 14 (XBD14) displays the highest bioactivity. Multiple genetic methods coupled with HRMS/MS analysis determined the major antifungal NP to be Fcl-29, a fabclavine derivative. Fcl-29 was found to effectively control FHB of wheat in the field test and demonstrated broad-spectrum antifungal activity against important pathogenic fungi. The production of Fcl-29 was dramatically improved by 33.82-fold with the combinatorial strategy of genetic engineering (1.66-fold) and fermentation engineering (20.39-fold). The exploration of a new biofungicide in global plant protection is now possible.

Disciplines :

Agriculture & agronomy

Author, co-author :

Li, Beibei ; Université de Liège - ULiège > TERRA Research Centre ; State Key Laboratory for Biology of Plant Diseases and Insect Pests/Key Laboratory of Control of Biological Hazard Factors (Plant Origin) for Agri-Product Quality and Safety, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100081, China

Language :

English

Title :

Identification of Fcl-29 as an Effective Antifungal Natural Product against Fusarium graminearum and Combinatorial Engineering Strategy for Improving Its Yield.

Publication date :

12 April 2023

Journal title :

Journal of Agricultural and Food Chemistry

ISSN :

0021-8561

eISSN :

1520-5118

Publisher :

American Chemical Society, United States

Volume :

Issue :

Pages :

5554 - 5564

Peer reviewed :

Peer Reviewed verified by ORBi

Additional URL :

https://pubs.acs.org/doi/pdf/10.1021/acs.jafc.2c09012

Funders :

CAAS - Chinese Academy of Agricultural Sciences
NSCF - National Natural Science Foundation of China

Funding text :

We thank the support from the National Natural Science Foundation of China (Grant No.31972327) and the Agricultural Science and Technology Innovation Program of CAAS (CAAS-ZDRW202011) and (CAAS-ZDRW202002). We also acknowledge the support from the Special Project on Regional Collaborative Innovation of Xinjiang Uygur Autonomous Region (Plan for Assisting Xinjiang with Science and Technology) (2021E02024), Natural Science Foundation of Xinjiang Uygur Autonomous Region (2022D01F069) and Key Research and Development Program Project of Xinjiang (2022B02044).

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